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    • 8. 发明申请
    • METHOD AND APPARATUS OF AUTOMATICALLY ISOLATING AND PURIFYING NUCLEIC ACID
    • 自动分离和纯化核酸的方法和装置
    • WO2005093053A1
    • 2005-10-06
    • PCT/JP2005/006530
    • 2005-03-28
    • FUJI PHOTO FILM CO., LTD.MORI, ToshihiroMAKINO, Yoshihiko
    • MORI, ToshihiroMAKINO, Yoshihiko
    • C12N15/00
    • C12N15/1006B01D15/14B01D15/163B01J20/26B01J20/262B01J20/265B01J20/28014B01J20/28033B01J20/28054C12N15/1017G01N30/32
    • A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration.
    • 提供了一种从含核酸样品自动分离和纯化核酸的方法,所述方法包括:将液体注入用于分离和纯化核酸的液体,所述核酸包括至少两个开口的至少两个开口 开口,其中所述盒包括具有所述至少两个开口并且包含核酸 - 吸附剂固相的容器; 通过由压力产生装置产生的压力差使液体通过核酸 - 吸附剂固相,用于产生容器内部和外部之间的压力差; 并且通过由压力产生装置产生的压力差将液体从容器的另一个开口排出到容器的外部,其中测量由压力产生装置在容器内部产生的压力,压力变化速度 并且基于测量的压力的值来计算压力变化加速度,并且通过使用测量压力中的至少一个的时间变化模式来确定来自容器的液体的排出的完成时间, 压力变化速度和压力变化加速度。