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    • 4. 发明申请
    • IMPROVED PLANT GERMPLASM
    • 改良植物GERMPLASM
    • WO1994003619A2
    • 1994-02-17
    • PCT/GB1993001605
    • 1993-07-29
    • ZENECA LIMITEDBRIGHT, Simon, William, JonathanGREENLAND, Andrew, JamesJEPSON, IanPAINE, Jacqueline, Ann, Mary
    • ZENECA LIMITED
    • C12N15/82
    • C12N9/88C12N15/8217C12N15/827
    • A recombinant plant genome contains a gene cascade such that it is incapable of producing a mature plant but requires the presence of a chemical inducer. The gene cascade includes a gene switch which is inducible by external application of a chemical inducer and which controls expression of a gene product which affects expression of a second gene in the genome. Survival or development of the plant is dependant upon either expression or non-expression of the second gene and application of the inducer, therefore, selects whether or not the plant develops. In one example the second gene encodes a cytotoxic molecule and expression of that gene is fatal to the plant. In another example the second gene encodes a desirable characteristic which may be excised selectively by applying or withholding chemical application. The second gene may be placed under control of a development regulated promoter.
    • 重组植物基因组包含基因级联,使得其不能产生成熟植物,但需要存在化学诱导物。 基因级联包括可通过外部施用化学诱导剂诱导的基因开关,其控制影响基因组中第二基因表达的基因产物的表达。 植物的存活或发育取决于第二基因的表达或不表达以及诱导物的应用,因此,选择植物是否发育。 在一个实例中,第二个基因编码细胞毒性分子,该基因的表达对植物是致命的。 在另一个实例中,第二基因编码可通过施用或扣留化学应用选择性切除的期望特征。 第二个基因可以置于发育调控启动子的控制之下。
    • 7. 发明申请
    • DNA CONSTRUCTS AND PLANTS INCORPORATING THEM
    • DNA构建与植物鉴定
    • WO1993021334A1
    • 1993-10-28
    • PCT/GB1993000764
    • 1993-04-13
    • ZENECA LIMITEDCADDICK, Mark, XavierGREENLAND, Andrew, JamesRIDDELL, Kay, VictoriaSCHUCH, Wolfgang, WalterTOMSETT, Arthur, Brian
    • ZENECA LIMITED
    • C12N15/82
    • C12N15/8237C12N15/8238
    • The invention relates to a chemically-inducible plant gene expression cassette and to plant cells transformed therewith. The expression cassette is suitable for use in any dicothyledonous or monocotyledonous plant. It comprises a first promoter operatively linked to a regulator sequence which encodes a regulator protein, and an inducible promoter operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene. The regulator sequence may be derived from the alcR gene, and the inducible promoter may be derived from the alcA gene promoter (both obtainable from Aspergillus nidulans) A chimeric promoter comprising an upstream regulatory region and a heterologous downstream transcription initiation region is also disclosed. The regulatory sequence may be derived from the alcA gene promoter.
    • 本发明涉及一种化学诱导型植物基因表达盒,并涉及用其转化的植物细胞。 表达盒适用于任何双子叶植物或单子叶植物。 其包含可操作地连接到编码调节蛋白的调节子序列的第一启动子和可操作地连接到靶基因的诱导型启动子,所述诱导型启动子在有效的外源诱导物存在下由调节蛋白激活,从而施用诱导物 导致靶基因的表达。 调节序列可以衍生自alcR基因,诱导型启动子可以衍生自alcA基因启动子(均可从构巢曲霉获得)。还公开了包含上游调节区和异源下游转录起始区的嵌合启动子。 调节序列可以来源于alcA基因启动子。