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1. 发明申请

WO2009043472A3 METHOD FOR THE MICROSCOPIC THREE-DIMENSIONAL REPRODUCTION OF A SAMPLE 审中-公开
标题翻译：方法样品的微观三维图像
公开(公告)号：WO2009043472A3
公开(公告)日：2009-07-30
申请号：PCT/EP2008007686
申请日：2008-09-16
申请人： ZEISS CARL MICROIMAGING GMBH , LIPPERT HELMUT , RADT BENNO , KEMPE MICHAEL , DIETRICH CHRISTIAN
发明人： LIPPERT HELMUT , RADT BENNO , KEMPE MICHAEL , DIETRICH CHRISTIAN
IPC分类号： G02B21/00 , G02B21/24 , G02B21/36
CPC分类号： G06T7/97 , G02B21/002 , G02B21/244 , G02B21/367 , G06T7/0022
摘要： The invention relates to methods for the three-dimensional reproduction of a sample, according to which image information from different depth planes of the sample is stored in a locally resolved manner and the three-dimensional image of the sample is then reconstructed from said image information. In a method of this type relating to selective plane illumination microscopy (SPIM), a reference structure is applied to the illumination light, at least one fluorescent reference object is positioned next to or in the sample, images of the reference structure for the illumination light, for the reference objects or of a sample structure that is suitable for use as a reference structure are captured from at least one detection direction and evaluated, the light layer is brought into an optimal position using the result, image information for the reference object and for the sample that has been captured from several detection directions is stored, the image information stored for the reference object is used to obtain transformation operators and these form the basis of the reconstruction of the three-dimensional reproduction of the sample from the image information that has been stored for the sample.
摘要翻译：本发明涉及一种用于样品的三维成像，在从不同的平面中的试样的深度的图像信息的空间分辨被存储的方法和随后从这些画面中的试样的三维图像重建地层。在这样的方法中，专用于选择性平面照明显微镜（SPIM）的制造方法是指，本发明提供了： - 所述照明光施加到参考结构， - 至少一个荧光基准对象旁边或在样品中被定位时，光片被带入结果的基础上的最佳位置， - - - 至少一个检测的方向的图像被记录下来并通过参考对象或多个参考对象的检测方向的图像信息的适当的参考结构样本结构的照明光的参考结构和样品的评价被存储， - 这些重构从所述图像信息的存储的样本的试样的三维图像获得基于使用从参考对象与变换算符所存储的图像信息。

2. 发明申请

WO2008131840A2 SAMPLE HOLDING SYSTEM FOR A MICROSCOPE 审中-公开
标题翻译：样品架在显微镜
公开(公告)号：WO2008131840A2
公开(公告)日：2008-11-06
申请号：PCT/EP2008002681
申请日：2008-04-04
申请人： ZEISS CARL MICROIMAGING GMBH , LIPPERT HELMUT , POWER CHRISTOPHER , DIETRICH CHRISTIAN , RADT BENNO
发明人： LIPPERT HELMUT , POWER CHRISTOPHER , DIETRICH CHRISTIAN , RADT BENNO
IPC分类号： G02B21/26
CPC分类号： G02B21/26
摘要： The invention relates to a sample holding system for a microscope, comprising a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. The sample holding system also comprises means for the translatory movement of the sample in relation to a detection objective of the microscope, and means for rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective. The means for rotating the sample in such a sample holding system comprise a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber, said drives transmitting the rotational movement to the holder, or the rotational movement is generated directly by the movement of the entire sample chamber.
摘要翻译：本发明涉及一种用于显微镜的样品保持器，其包括填充有浸没液体样品室中的塔，在其中一个嵌入透明灌封介质样品位于一个保持器，其特征在于，具有上开口的样品室，用于向检测目标的相对的样品的翻译显微镜，以及用于将所述样品围绕旋转基本垂直的轴，其位于包含检测目标的一个非零角度的光轴的平面。在这样的样品保持器，用于旋转驱动用磁耦合或样品室上方转动样品的装置置于带和/或齿轮的旋转驱动器，其传输所述旋转移动到保持架，或旋转运动是直接在整个的移动样品室达到。

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