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    • 1. 发明申请
    • SAMPLE HOLDING SYSTEM FOR A MICROSCOPE
    • 样品架在显微镜
    • WO2008131840A2
    • 2008-11-06
    • PCT/EP2008002681
    • 2008-04-04
    • ZEISS CARL MICROIMAGING GMBHLIPPERT HELMUTPOWER CHRISTOPHERDIETRICH CHRISTIANRADT BENNO
    • LIPPERT HELMUTPOWER CHRISTOPHERDIETRICH CHRISTIANRADT BENNO
    • G02B21/26
    • G02B21/26
    • The invention relates to a sample holding system for a microscope, comprising a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. The sample holding system also comprises means for the translatory movement of the sample in relation to a detection objective of the microscope, and means for rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective. The means for rotating the sample in such a sample holding system comprise a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber, said drives transmitting the rotational movement to the holder, or the rotational movement is generated directly by the movement of the entire sample chamber.
    • 本发明涉及一种用于显微镜的样品保持器,其包括填充有浸没液体样品室中的塔,在其中一个嵌入透明灌封介质样品位于一个保持器,其特征在于,具有上开口的样品室,用于向检测目标的相对的样品的翻译 显微镜,以及用于将所述样品围绕旋转基本垂直的轴,其位于包含检测目标的一个非零角度的光轴的平面。 在这样的样品保持器,用于旋转驱动用磁耦合或样品室上方转动样品的装置置于带和/或齿轮的旋转驱动器,其传输所述旋转移动到保持架,或旋转运动是直接在整个的移动 样品室达到。
    • 2. 发明申请
    • METHOD FOR THE MICROSCOPIC THREE-DIMENSIONAL REPRODUCTION OF A SAMPLE
    • 方法样品的微观三维图像
    • WO2009043472A3
    • 2009-07-30
    • PCT/EP2008007686
    • 2008-09-16
    • ZEISS CARL MICROIMAGING GMBHLIPPERT HELMUTRADT BENNOKEMPE MICHAELDIETRICH CHRISTIAN
    • LIPPERT HELMUTRADT BENNOKEMPE MICHAELDIETRICH CHRISTIAN
    • G02B21/00G02B21/24G02B21/36
    • G06T7/97G02B21/002G02B21/244G02B21/367G06T7/0022
    • The invention relates to methods for the three-dimensional reproduction of a sample, according to which image information from different depth planes of the sample is stored in a locally resolved manner and the three-dimensional image of the sample is then reconstructed from said image information. In a method of this type relating to selective plane illumination microscopy (SPIM), a reference structure is applied to the illumination light, at least one fluorescent reference object is positioned next to or in the sample, images of the reference structure for the illumination light, for the reference objects or of a sample structure that is suitable for use as a reference structure are captured from at least one detection direction and evaluated, the light layer is brought into an optimal position using the result, image information for the reference object and for the sample that has been captured from several detection directions is stored, the image information stored for the reference object is used to obtain transformation operators and these form the basis of the reconstruction of the three-dimensional reproduction of the sample from the image information that has been stored for the sample.
    • 本发明涉及一种用于样品的三维成像,在从不同的平面中的试样的深度的图像信息的空间分辨被存储的方法和随后从这些画面中的试样的三维图像重建地层。 在这样的方法中,专用于选择性平面照明显微镜(SPIM)的制造方法是指,本发明提供了: - 所述照明光施加到参考结构, - 至少一个荧光基准对象旁边或在样品中被定位时, 光片被带入结果的基础上的最佳位置, - - - 至少一个检测的方向的图像被记录下来并通过参考对象或多个参考对象的检测方向的图像信息的适当的参考结构样本结构的照明光的参考结构和样品的评价 被存储, - 这些重构从所述图像信息的存储的样本的试样的三维图像获得基于使用从参考对象与变换算符所存储的图像信息。
    • 3. 发明申请
    • MICROSCOPE
    • 显微镜
    • WO2009080210A2
    • 2009-07-02
    • PCT/EP2008010455
    • 2008-12-10
    • ZEISS CARL MICROIMAGING GMBHLIPPERT HELMUTWOLLESCHENSKY RALF
    • LIPPERT HELMUTWOLLESCHENSKY RALF
    • G02B21/10G02B21/16G02B27/58
    • G02B21/06G02B21/10G02B21/16G02B26/0816G02B27/0911G02B27/58
    • The invention relates to a microscope, comprising an imaging lens (4) for imaging a sample (3) on a detector and means for illuminating the sample (3) with a light layer (7) in the focus plane of the imaging lens (4), comprising an illumination source (1) emitting coherent light. In a microscope of said kind, the means for illumination comprise a Bessel lens, creating at least two plane waves (6) from the light beam (5) and predefining propagation directions for the plane waves (6), wherein the propagation direction of each of the plane waves (6) forms an acute angle with the focus plane, the amount of the angle being the same for each of the plane waves (6), such that the plane waves (6) constructively interfere in the focus place, thus creating a light layer (7). In a similar way, the means for illumination can also comprise an optical element, with which a rotationally symmetrical Bessel beam is created from the light beam (5), for dynamically creating a light layer (7).
    • 本发明涉及一种包括成像透镜,用于到检测器的试样(3)成像(4)的显微镜和用于与成像透镜(4)的焦平面中的光片(7)照射所述样品(3),包括:相干光 辐射照射源(1)。 在这样的显微镜,用于照明的所述装置包括一个贝塞尔光学,(5)的至少两个平面波(6)和所述平面波(6)传播方向假装每个平面波(6)的与所述焦平面中的传播方向,其从光束产生 每一个都包括一个锐角,其量为每个平面波的(6)是相等的,从而使干扰平面波(6)在结构上在焦平面,从而提供光片(7)被产生。 类似地,与从所述光束(5),产生用于照明的装置,光学元件的旋转对称的贝塞尔光束,用于动态地生成光片(7)包括。