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2. 发明申请

WO1995029244A1 METHOD TO INCREASE REGULATORY MOLECULE PRODUCTION 审中-公开
标题翻译：增加法规分子生产的方法
公开(公告)号：WO1995029244A1
公开(公告)日：1995-11-02
申请号：PCT/US1995004903
申请日：1995-04-21
申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION
发明人： WISCONSIN ALUMNI RESEARCH FOUNDATION , MALTER, James, S.
IPC分类号： C12N15/67
CPC分类号： C07K14/535 , C07K14/5403 , C12N15/67
摘要： The present invention provides a method to increase the production of a regulatory molecule, i.e., a molecule that regulates cell behavior, such as a cytokine or a protooncogene, in population of transfected cells, whether normal (resting or activated) or tumor cells. The method involves mutating a native or "wild type" cDNA sequence that encodes an mRNA sequence for a regulatory molecule to form a mutant cDNA sequence capable of producing a more stable mRNA sequence. Specifically, the method involves mutating a wild type cDNA sequence that encodes an unstable mRNA sequence for the regulatory molecule, wherein the mRNA includes a 3' untranslated region having a destabilizing element comprising an AUUUA sequence, to form a mutant cDNA sequence capable of producing a more stable mRNA sequence, wherein the AUUUA sequence is replaced by AUGUA, AUAUA, GUGUG, AGGGA, GAGAG, or a combination thereof; and transfecting a cell population with the mutant cDNA sequence so that the production of the regulatory molecule is enhanced by the cells.
摘要翻译：本发明提供了在正常（休息或活化）或肿瘤细胞的转染细胞群体中增加调节分子即调节细胞行为的分子（例如细胞因子或原癌基因）的产生的方法。该方法包括突变编码用于调节分子的mRNA序列的天然或“野生型”cDNA序列，以形成能够产生更稳定的mRNA序列的突变cDNA序列。具体地说，该方法包括突变编码调节分子不稳定mRNA序列的野生型cDNA序列，其中mRNA包含具有包含AUUUA序列的不稳定元件的3'非翻译区，以形成能够产生更稳定的mRNA序列，其中AUUUA序列被AUGUA，AUAUA，GUGUG，AGGGA，GAGAG或其组合替代; 并用突变cDNA序列转染细胞群，从而细胞增强调节分子的产生。

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