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1. 发明申请

WO2005043112A3 APPARATUS AND METHOD FOR EDMAN DEGRADATION ON A MICROFLUIDIC DEVICE UTILIZING AN ELECTROOSMOTIC FLOW PUMP 审中-公开
公开(公告)号：WO2005043112A3
公开(公告)日：2005-05-12
申请号：PCT/US2004/032145
申请日：2004-09-30
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION , TIMPERMAN, Aaron, T.
发明人： TIMPERMAN, Aaron, T.
IPC分类号： G01N33/553 , G01N33/551 , C12M1/34
摘要： The present invention comprises an electroosmotic flow pump with both anion (43) and cation (41) exchange beads packed in separate channels (5, 9) that pump towards an intersection (8). Combining the two flow streams results in higher flowrates for the pump and allows operation of the pump over a wide pH range. The pump can be used to deliver solutions ranging from a pH of about 2 to about 12. In a preferred embodiment, the electroosmotic pump of the present invention is fabricated on a microfluidic device (55) capable of Edman degradation. In a preferred embodiment of the present invention, the beads (41, 43) are immobilized in the channels (5, 9) using weirs (7) and membranes, eliminating the need for frits. The pump may be comprised of capillaries (27, 29, 31). Additionally, the electroosmotic flow pump of the present invention may be integrated into an Integrated Microfluidic Proteome Analysis System.

2. 发明申请

WO2007016432A2 APPARATUS AND METHOD FOR COUPLING MICROFLUIDIC SYSTEMS WITH A MASS SPECTROMETER UTILIZING RAPID VOLTAGE SWITCHING 审中-公开
标题翻译：利用快速电压切换将微流体系统与质谱仪耦合的装置和方法
公开(公告)号：WO2007016432A2
公开(公告)日：2007-02-08
申请号：PCT/US2006/029600
申请日：2006-07-28
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION , TIMPERMAN, Aaron, T. , LENKE, James , RAZUNGUZWA, Trust
发明人： TIMPERMAN, Aaron, T. , LENKE, James , RAZUNGUZWA, Trust
IPC分类号： G01N33/50
CPC分类号： H01J49/165 , B01L3/5027 , C07K1/26 , H01J27/04 , H01J49/0018
摘要： The invention relates to an apparatus for coupling microfluidic systems with electrospray ionization mass spectrometry utilizing a hydrodynamic flow. The invention also relates to a method of preventing a sample in a main channel (25) of a microfluidic device (11) from migrating down either a first side channel (31) or a second side channel (33) by rapidly alternating a voltage being applied to the first side channel (31) and the second side channel (33).
摘要翻译：本发明涉及一种利用流体动力学流动将微流体系统与电喷雾电离质谱联用的装置。本发明还涉及一种防止微流体装置（11）的主通道（25）中的样品通过快速交替变化的电压向第一侧通道（31）或第二侧通道（33）向下迁移的方法应用于第一侧通道（31）和第二侧通道（33）。

3. 发明申请

WO2004077017A2 APPARATUS AND METHOD FOR ON-CHIP CONCENTRATION USING A MICROFLUIDIC DEVICE WITH AN INTEGRATED ULTRAFILTRATION MEMBRANE STRUCTURE 审中-公开
标题翻译：使用具有一体化超滤膜结构的微流体装置的芯片浓度的装置和方法
公开(公告)号：WO2004077017A2
公开(公告)日：2004-09-10
申请号：PCT/US2004/005212
申请日：2004-02-23
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION , TIMPERMAN, Aaron, T.
发明人： TIMPERMAN, Aaron, T.
IPC分类号： G01N
CPC分类号： G01N27/44791 , B01D57/02 , B01D61/18 , B01D61/425 , B01L3/5027 , B01L3/502753 , B01L2200/0631 , B01L2300/0681 , B01L2300/0816 , B01L2300/0887 , B01L2400/0418 , B82Y30/00 , C12Q1/00 , C12Q1/37 , C12Q1/42 , G01N1/40 , G01N2001/4038
摘要： The present invention provides a microfluidic device 5 capable of reacting an enzyme or other agent with a substantially purified polypeptide. In one embodiment of the present invention, the microfluic device 5 comprises a plurality of reaction channels 8 wherein the substantially purified polypeptide is delivered to a reaction channel 8. Once confined within the reaction channel 8, the substantially purified polypeptide engages the enzyme or agent and produces a reaction product. In a preferred embodiment of the present invention, the reaction product is concentrated at a charged membrane 101,103 prior to being removed from the microfluidic device 5. Additionally, the present invention provides a method of concentrating a positive analyte at a positively charged membrane 103 and a negative analyte at a negatively charged membrane 101. Once concentrated, the analytes may be removed from the microfluidic device 5.
摘要翻译：本发明提供能够使酶或其它试剂与基本上纯化的多肽反应的微流体装置5。在本发明的一个实施方案中，微流体装置5包括多个反应通道8，其中基本上纯化的多肽被递送至反应通道8.一旦限制在反应通道8内，基本上纯化的多肽与酶或试剂接合，产生反应产物。在本发明的优选实施方案中，在从微流体装置5中除去之前，将反应产物在带电膜101,103处浓缩。另外，本发明提供了一种将阳性分析物浓缩在带正电膜103和一旦浓缩，分析物可以从微流体装置5去除。

4. 发明申请

WO2007002141A3 APPARATUS AND METHOD FOR COUPLING MICROFLUIDIC SYSTEMS WITH ELECTROSPRAY IONIZATION MASS SPECTROMETRY UTILIZING A HYDRODYNAMIC FLOW RESTRICTOR 审中-公开
公开(公告)号：WO2007002141A3
公开(公告)日：2007-01-04
申请号：PCT/US2006/024065
申请日：2006-06-20
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION , TIMPERMAN, Aaron, T.
发明人： TIMPERMAN, Aaron, T.
IPC分类号： C12M1/34
摘要： A microfluidic device and method are disclosed. The microfludic device has input and output channels and a main channel engaging the input channel to the output channel. A hydrodynamic flow restrictor is positioned in the main channel, which substantially negates the hydrodynamic backpressure in the main channel to the extent that low electroosmotic flow may be utilized. A make-up flow Channel engages the main channel at a position between the hydrodynamic flow restrictor and the output channel.

5. 发明申请

WO2004059283A3 APPARATUS AND METHOD FOR EDMAN DEGRADATION USING A MICROFLUIDIC SYSTEM 审中-公开
公开(公告)号：WO2004059283A3
公开(公告)日：2004-07-15
申请号：PCT/US2003/040387
申请日：2003-12-18
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION
发明人： TIMPERMAN, Aaron, T.
IPC分类号： G01N35/00
摘要： An apparatus and method for Edman degradation using a microfluidic system to identify and characterize peptides is disclosed. A microfluidic device comprises an entrance channel through which a substantially purified polypeptide is accepted, a reaction channel engaging the entrance channel wherein the substantially purified polypeptide is digested, producing a digestion product, a reagent reservoir engaging the reaction channel, the reagent reservoir capable of delivering a reagent to the reaction channel, and an exit channel extending from the reaction channel, wherein the digestion product travels through the exit channel upon leaving the reaction channel. Protein digestion on the device comprises delivering a substantially purified polypeptide to a reaction channel, confining the polypeptide in the reaction channel, digesting the polypeptide in the reaction channel producing a digestion product, and removing the digestion product from the reaction channel, wherein the last two steps are repeated until the polypeptide is substantially digested.

6. 发明申请

WO2004076497A2 APPARATUS AND METHOD OF USING BI-DIRECTIONAL CAPILLARY ELECTROPHORESIS 审中-公开
标题翻译：装置和使用双向毛细管电泳的方法
公开(公告)号：WO2004076497A2
公开(公告)日：2004-09-10
申请号：PCT/US2004/005451
申请日：2004-02-23
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION , TIMPERMAN, Aaron, T.
发明人： TIMPERMAN, Aaron, T.
IPC分类号： C08F
CPC分类号： G01N27/44756
摘要： The present invention provides an apparatus and method of utilizing bi-directional capillary electrophoresis ("CE") device 9. In one embodiment, cations are drawn into a first uncharged channel 23 towards a negative electrode 27 and anions are drawn into a second uncharged channel 24 towards a positive electrode 25. The uncharged channels 23,24 allows for minimal interaction between the sample and the walls of the channel 23,24 which allows for minimal sample loss. The present invention also provides a method of separating anions from cations. The method comprises delivering the mixture to a bi-directional CE device 9. Following delivery, cations are drawn into a first uncharged channel 23 towards a negative electrode 27 and anions are drawn into a second uncharged channel 24 towards a positive electrode 25. In one aspect of the present invention, the bi-directional CE device 9 engages a microfluidic proteome analysis system 31.
摘要翻译：本发明提供一种使用双向毛细管电泳（“CE”）装置9的装置和方法。在一个实施方案中，将阳离子吸入第一不带电通道23朝向负极27，阴离子被吸入第二不带电通道 24通过正电极25.不带电的通道23,24允许样品与通道23,24的壁之间的最小的相互作用，其允许最小的样品损失。本发明还提供了一种从阳离子中分离阴离子的方法。该方法包括将混合物输送到双向CE器件9.在传送之后，阳离子被吸入朝向负极27的第一不带电通道23中，阴离子被吸入朝向正电极25的第二不带电通道24中。在一个本发明的方面，双向CE器件9接合微流体蛋白质组分析系统31。

7. 发明申请

WO2003044221A1 MICROFLUIDIC SYSTEM FOR PROTEOME ANALYSIS 审中-公开
标题翻译：微生物分析系统
公开(公告)号：WO2003044221A1
公开(公告)日：2003-05-30
申请号：PCT/US2002/033319
申请日：2002-10-18
申请人： WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION , TIMPERMAN, Aaron, T.
发明人： TIMPERMAN, Aaron, T.
IPC分类号： C12Q1/37
CPC分类号： G01N27/44791 , B01L3/5027 , B01L3/502707 , B01L3/502753 , B01L2200/10 , B01L2400/0415 , G01N27/44717 , G01N27/44743 , G01N27/44773 , G01N33/6803
摘要： The invention provides a microfluidic system and method to rapidly analyze large number of compounds or complex mixtures of compounds, particularly, low abundance cellular proteins involved in cell signaling pathways. In one aspect, an integrated microfluidic system (1) comprises an upstream separation module (2) (preferably, a multi-dimensional separation device (2a)), a microfluidic device (5) for on-device protein digestion of substantially separated proteins received from the upstream separation module (2), a downstream separation module (14) for separating digestion products of said proteins, a peptide analysis module (17) and a processor (18) for determining the amino acid sequence of said proteins. Preferably, the integrated microfluidic system (1) comprises an interfacing microfluidic device (5i) between the downstream separation module (14) and the peptide analysis module (17).
摘要翻译：本发明提供了一种微流体系统和方法，用于快速分析大量化合物或化合物的复合混合物，特别是涉及细胞信号通路的低丰度细胞蛋白质。在一个方面，集成微流体系统（1）包括上游分离模块（2）（优选地，多维分离装置（2a）），用于装置上蛋白质消化基本上分离的蛋白质的微流体装置（5）来自上游分离模块（2）的用于分离所述蛋白质的消化产物的下游分离模块（14），用于测定所述蛋白质的氨基酸序列的肽分析模块（17）和处理器（18）。优选地，集成微流体系统（1）包括在下游分离模块（14）和肽分析模块（17）之间的界面微流体装置（5i）。

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