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2. 发明申请

WO2004108260A2 DEVICES AND METHODS FOR BIOMATERIAL PRODUCTION 审中-公开
标题翻译：用于生物体生产的装置和方法
公开(公告)号：WO2004108260A2
公开(公告)日：2004-12-16
申请号：PCT/US2004/016806
申请日：2004-05-27
申请人： ADVISYS, INC. , HEBEL, Henry , RAMAKRISHNAN, Sriram , GONZALEZ, Hugo , DARNELL, Jeff
发明人： HEBEL, Henry , RAMAKRISHNAN, Sriram , GONZALEZ, Hugo , DARNELL, Jeff
IPC分类号： B01F3/04
CPC分类号： C12N15/1003 , B01F13/0255 , B03D2203/003 , C12M47/06 , C12M47/10 , C12N1/06 , C12N1/066 , C12N15/101
摘要： An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipiation buffer and floats compacted precipitated cellular material.
摘要翻译：从细胞中分离生物制品如质粒DNA的装置和方法。该方法包括以受控的方式裂解细胞，从含有感兴趣的细胞成分的流体裂解物中分离出不溶性组分，然后进行膜色谱技术纯化感兴趣的细胞组分。该方法利用独特的裂解装置，离子交换以及任选的以胶囊形式的疏水相互作用层析膜和超滤。该方法可以应用于从细胞来源提取的任何生物制品。该方法使用裂解装置，包括高剪切，低停留时间混合器，有利地将细胞悬浮液与裂解溶液混合，使杂质变性的保持时间和将裂解细胞轻轻地充分混合的空气鼓泡混合器中和/沉淀缓冲液和漂浮压实的沉淀的细胞材料。

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