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1. 发明申请

WO1989010690A1 METHOD FOR ELECTROFUSING BIOLOGICAL PARTICLES TO TISSUES 审中-公开
标题翻译：电组织生物颗粒的方法
公开(公告)号：WO1989010690A1
公开(公告)日：1989-11-16
申请号：PCT/US1989001774
申请日：1989-04-27
申请人： UNIVERSITY OF SOUTH FLORIDA , GRASSO, Robert, J.
发明人： UNIVERSITY OF SOUTH FLORIDA
IPC分类号： A01K45/00
CPC分类号： C12N13/00 , C12N5/12
摘要： This invention relates to methods for electrofusing biological particles to cells of animal or plant tissues in vitro, in situ, or vivo. Selected biological particles are placed in contact with cells of a selected tissue and fused to cells in the tissue by means of an electric field. The method of this invention may be used to produce animals and plants characterized by features and properties that differ from those typically displayed by the native species. One embodiment of the methods of this invention has been used to produce an animal model for ocular gonorrhea by electrofusing human cells with functional receptors for the human bacterial pathogen Neisseria Gonorrhoeae to epithelial cells in the histologically intact superficial corneal tissue of living rabbits. The methods of this invention may be used to produce other animal or plant models for the study of receptor-mediated processes, including infectivity studies required for the development of vaccines effective to protect against or lessen the severity of infections caused by microbial pathogens. This is achieved by electrofusing cells with membrane receptors for a pathogen to cells of tissues of plants or animals that are normally not susceptible to infection by that pathogen. Other applications are disclosed.
摘要翻译：本发明涉及在体外，原位或体内将生物颗粒电气化到动物或植物组织细胞的方法。将选定的生物颗粒置于与选定组织的细胞接触并通过电场与组织中的细胞融合。本发明的方法可以用于产生特征在于与天然物种通常显示的特征和性质不同的特征和特征的动物和植物。本发明方法的一个实施方案已经用于通过将人细胞与人细菌性病原体淋病奈瑟氏球菌的功能受体电融合到活的兔的组织学上完整的表浅角膜组织中的上皮细胞上来产生眼淋病动物模型。本发明的方法可用于产生用于研究受体介导的过程的其它动物或植物模型，包括开发有效防止或减轻由微生物病原体引起的感染的严重性的疫苗所需的感染性研究。这通过将具有病原体的膜受体的细胞与通常不易被该病原体感染的植物或动物的组织细胞电融合而实现。公开了其他应用。

2. 发明申请

WO1998017782A1 METHOD FOR PRODUCING TRANSGENIC ANIMAL MODELS WITH MODULATED PHENOTYPE AND ANIMALS PRODUCED THEREFROM 审中-公开
标题翻译：用于生产具有调节性反应动物和动物的转基因动物模型的方法
公开(公告)号：WO1998017782A1
公开(公告)日：1998-04-30
申请号：PCT/US1997018524
申请日：1997-10-15
申请人： UNIVERSITY OF SOUTH FLORIDA , DUFF, Karen , HARDY, John
发明人： UNIVERSITY OF SOUTH FLORIDA
IPC分类号： C12N05/00
CPC分类号： C12N15/8509 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2217/075 , A01K2227/105 , A01K2267/0312 , A61K49/0008 , C07K14/4711
摘要： A method of preparing a transgenic animal model with enhanced, accelerated pathology for Alzheimer's Disease (AD) and the transgenic animal made by the method are disclosed. The method includes producing an F1 generation by crossing a first and second transgenic parent each carrying a different expressible transgene for differing aspects of the same desired phenotype associated with AD pathology. The offsprings of the F1 generation are then screened and those which carry a transgene from each parental transgenic animal resulting in an enhanced pathology for Alzheimer's Disease are selected. In a preferred embodiment the AD-associated pathology is for amyloid accumulation. In an embodiment a mutant presenilin transgene and a transgene for a mutant amyloid precursor protein are used. In a further embodiment the mutant presenilin transgene is the PS1 M146L mutation and the mutant amyloid precursor protein transgene is the Swedish mutation (app695 isoform containing a K670N, M671L mutation (app770 numbering )).
摘要翻译：公开了一种制备具有阿尔茨海默病（AD）增强，加速病理学和通过该方法制备的转基因动物的转基因动物模型的方法。该方法包括通过与携带不同可表达转基因的第一和第二转基因亲本杂交产生F1代，用于与AD病理学相关的相同所需表型的不同方面。然后筛选F1代的后代，并选择携带来自每个亲代转基因动物的转基因导致阿尔茨海默病的增强病理学的后代。在优选实施方案中，AD相关病理学用于淀粉样蛋白积聚。在一个实施方案中，使用突变型早老素转基因和用于突变淀粉样蛋白前体蛋白的转基因。在另一个实施方案中，突变型早老素转基因是PS1 M146L突变，突变淀粉样蛋白前体蛋白转基因是瑞典突变（含有K670N，M671L突变（app770编号）的app695同种型））。

3. 发明申请

WO1997015689A1 METHOD TO DETECT POLYMORPHISM IN THE S182 GENE 审中-公开
标题翻译：检测S182基因多态性的方法
公开(公告)号：WO1997015689A1
公开(公告)日：1997-05-01
申请号：PCT/US1996017132
申请日：1996-10-25
申请人： WASHINGTON UNIVERSITY SCHOOL OF MEDICINE , UNIVERSITY OF SOUTH FLORIDA , HARDY, John, A. , GOATE, Alison, M.
发明人： WASHINGTON UNIVERSITY SCHOOL OF MEDICINE , UNIVERSITY OF SOUTH FLORIDA
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6883 , C12Q1/683 , C12Q1/6869 , C12Q2600/156
摘要： A method of detecting polymorphisms in the S182 gene by detecting mutations in the S182 gene using selected mismatched PCR primers comprising sequences derived from intronic sequences of the S182 gene is provided. A method of identifying an individual susceptible to late onset Alzheimer's Disease is also provided.
摘要翻译：提供了通过使用包含来自S182基因的内含子序列的序列的选择的错配PCR引物检测S182基因中的突变来检测S182基因中的多态性的方法。还提供了鉴定易患迟发性阿尔茨海默病的个体的方法。

4. 发明申请

WO1997007826A1 IN VIVO ELECTROPORATION OF CELLS 审中-公开
标题翻译：细胞生物电泳
公开(公告)号：WO1997007826A1
公开(公告)日：1997-03-06
申请号：PCT/US1996013591
申请日：1996-08-26
申请人： CBR LABORATORIES, INC. , UNIVERSITY OF SOUTH FLORIDA
发明人： CBR LABORATORIES, INC. , UNIVERSITY OF SOUTH FLORIDA , NICOLAU, Claude, Y. , HELLER, Richard , ATKIN, Andrew , GILBERT, Richard , JAROSZESKI, Mark
IPC分类号： A61K48/00
CPC分类号： C12N15/89 , A61K48/00
摘要： A method of treating living mammalians in vivo is carried out by introducing foreign nucleic acid by electroporation into cells in vivo to cause suppression or expression of at least one protein in vivo. The method can be carried out to alter genetic characteristics of cells in vivo.
摘要翻译：通过将外源核酸通过电穿孔引入体内的细胞来进行体内活体哺乳动物的治疗方法，以在体内引起至少一种蛋白质的抑制或表达。该方法可以在体内改变细胞的遗传特征。

5. 发明申请

WO1997003999A1 MUTANT S182 GENES 审中-公开
标题翻译： MUTANT S182基因
公开(公告)号：WO1997003999A1
公开(公告)日：1997-02-06
申请号：PCT/US1996011065
申请日：1996-06-26
申请人： WASHINGTON UNIVERSITY SCHOOL OF MEDICINE , UNIVERSITY OF SOUTH FLORIDA , GOATE, Alison, M. , HARDY, John, A.
发明人： WASHINGTON UNIVERSITY SCHOOL OF MEDICINE , UNIVERSITY OF SOUTH FLORIDA
IPC分类号： C07H21/04
CPC分类号： C12Q1/6883 , A01K2217/05 , C07K14/4711 , C12Q2600/156
摘要： The invention provides novel mutant S182 sequences, methods of diagnosing Alzheimer's disease using these novel mutant S182 genes, a model system for Alzheimer's disease comprising a mutant S182 gene, and methods of identifying mutations in genes homologous to the S182 gene.
摘要翻译：本发明提供了新的突变S182序列，使用这些新型突变体S182基因诊断阿尔茨海默病的方法，包含突变S182基因的阿尔茨海默病模型系统，以及鉴定与S182基因同源的基因突变的方法。

6. 发明申请

WO1996024837A2 SPECTROPHOTOMETRIC METHOD AND APPARATUS FOR THE CHARACTERIZATION OF BLOOD, BLOOD TYPES, AND OTHER BODILY FLUIDS 审中-公开
标题翻译：用于表征血液，血液类型和其他体液的分光光度法和装置
公开(公告)号：WO1996024837A2
公开(公告)日：1996-08-15
申请号：PCT/US1996001676
申请日：1996-02-07
申请人： UNIVERSITY OF SOUTH FLORIDA
发明人： UNIVERSITY OF SOUTH FLORIDA , GARCIA-RUBIO, Luis, Humberto , BAYONA, Manuel , POTTER, Robert , LEPARC, German
IPC分类号： G01N21/47
CPC分类号： G01N33/80 , G01N21/31 , G01N21/49
摘要： A method and apparatus for determining the type of a blood sample are provided wherein a turbidity spectrum of the blood sample is collected over a predetermined wavelength range, from which is calculated an extinction spectrum. This extinction sprectrum is then compared with a set of control spectra collected from control blood samples having known blood types, from which the type of the blood sample can be determined. A further method is provided for detecting the presence of a substance in a bodily fluid sample, the substance having a size in the range of generally 0.5 to 20 mu m. Exemplary substances that could be of interest to detect include, but are not limited to, hemoglobin, bilirubin, red blood cell antigens, microorganisms, and viruses. This embodiment includes the additional step of deconvoluting the extinction spectrum to obtain a particle size distribution for comparison with a database of control samples.
摘要翻译：提供了一种用于确定血液样本类型的方法和装置，其中在预定波长范围内收集血液样本的浊度谱，由此计算消光谱。然后将该灭绝性脊椎动物与从具有已知血型的对照血液样品收集的一组控制光谱进行比较，从该血液样品中可以确定血液样品的类型。提供了用于检测物质在体液样品中的存在的另一种方法，该物质的大小通常在0.5至20μm的范围内。可能感兴趣的检测的示例性物质包括但不限于血红蛋白，胆红素，红细胞抗原，微生物和病毒。该实施方案包括解卷积消光谱以获得粒度分布的附加步骤，用于与对照样品的数据库进行比较。

7. 发明申请

WO1994002623A1 BLOOD LEVELS OF CCK PEPTIDES RELATIVE TO PANIC DISORDER TREATMENT 审中-公开
标题翻译： CCK PEPTIDES相对于泛滥障碍治疗的血液水平
公开(公告)号：WO1994002623A1
公开(公告)日：1994-02-03
申请号：PCT/US1993006650
申请日：1993-07-14
申请人： UNIVERSITY OF SOUTH FLORIDA
发明人： UNIVERSITY OF SOUTH FLORIDA , THOMAS, Thomas, N. , SHEEHAN, David, V. , TALBOT, Janet, D.
IPC分类号： C12P11/00
CPC分类号： A61K31/135 , A61K31/505 , A61K31/55 , G01N33/6893 , G01N2800/301 , G01N2800/52 , Y10T436/106664
摘要： A method of treating a patient having a panic disorder, the patient having an elevated CCK peptide plasma level, by lowering the plasma CCK peptide level of the patient. A further method provides a diagnosis of panic disorder in a patient by detecting if that patient's plasma contains elevated CCK peptide levels. A further method determines the efficacy of the drug for the treatment of panic disorder by detecting the ability of the drug to lower elevated CCK peptide levels in a model for panic disorder. Additionally, a method of dosing a patient having elevated CCK peptide levels with an antipanic disorder drug is characterized by administering the drug to a patient and monitoring the lowering of the elevated plasma CCK peptide levels of the patient.
摘要翻译：通过降低患者的血浆CCK肽水平，治疗患有惊恐障碍的患者的方法，该患者具有升高的CCK肽血浆水平。另一种方法通过检测患者的血浆是否含有升高的CCK肽水平来提供对患者恐慌症的诊断。另一种方法通过检测药物在惊恐障碍模型中降低升高的CCK肽水平的能力来确定药物治疗恐慌症的功效。此外，给予具有升高的CCK肽水平的患者的方法通过给抗病毒药物给药，其特征在于向患者施用药物并监测患者的升高的血浆CCK肽水平的降低。

8. 发明申请

WO1992014543A1 UNIMOLECULAR MICELLES AND METHOD OF MAKING THE SAME 审中-公开
标题翻译：非分子细胞及其制备方法
公开(公告)号：WO1992014543A1
公开(公告)日：1992-09-03
申请号：PCT/US1992001310
申请日：1992-02-18
申请人： UNIVERSITY OF SOUTH FLORIDA
发明人： UNIVERSITY OF SOUTH FLORIDA , NEWKOME, George, R. , MOOREFIELD, Charles, N.
IPC分类号： B01J13/00
CPC分类号： C08G83/003 , A61K47/6907 , B01F17/0028 , C07C17/16 , C07C43/166 , C08G61/00 , C08G85/00 , Y10S516/01 , Y10S516/06 , C07C19/075
摘要： In accordance with the present invention there is provided a method of making a cascade polymer including the steps of alkylating the branches of a multi-branch core alkyne building block including multiple ether side chains and simultaneously reducing the alkyne triple bonds and deprotecting to form a multi-hydroxyl terminated, multi-branched all alkyl polymer. The inventive method results in the formation of a unimolecular micelle consisting essentially of alkyl carbon.

9. 发明申请

WO1990002612A1 SUPERCRITICAL FLUID-AIDED TREATMENT OF POROUS MATERIALS 审中-公开
标题翻译：超临界流体辅助多孔材料处理
公开(公告)号：WO1990002612A1
公开(公告)日：1990-03-22
申请号：PCT/US1989003129
申请日：1989-07-14
申请人： UNIVERSITY OF SOUTH FLORIDA
发明人： UNIVERSITY OF SOUTH FLORIDA , SUNOL, Aydin, K.
IPC分类号： B05D03/00
CPC分类号： B27K5/007 , B05D1/18 , B05D7/06 , B05D2203/20 , B05D2401/90 , B27K3/15 , C08L33/12 , C08L97/02 , Y02P20/544
摘要： Method and process using a supercritical fluid, with or without the aid of entrainers to solubilize a monomer, monomer mixture or polymer which may or may not include additives and entrainers; to carry the supercritical solvent mixture (40) thereby created into the wood matrix (34) via a pressure tank (12); to remove extractives from the wood; to precipitate the monomer or polymer within the wood; and to polymerize the monomer in situ in the wood.

10. 发明申请

WO1997033470A1 METHOD AND MEDIA FOR ENHANCING VIABILITY, MATURATION, AND CRYOPRESERVATION OF CELLS 审中-公开
标题翻译：用于增强细胞的可变性，成熟和CRYOPRESERVATION的方法和媒介
公开(公告)号：WO1997033470A1
公开(公告)日：1997-09-18
申请号：PCT/US1997003911
申请日：1997-03-11
申请人： UNIVERSITY OF SOUTH FLORIDA , SANBERG, Paul, R. , OTHBERG, Agneta , CAMERON, Don, F. , SAPORTA, Samuel , BORLONGAN, Cesario, V.
发明人： UNIVERSITY OF SOUTH FLORIDA
IPC分类号： A01N01/00
CPC分类号： C12N5/0683 , A01N1/0226 , A61K35/12 , A61K2035/122 , C12N5/0619 , C12N2502/246
摘要： A method to increase viability, number, survival and maturation of cells for transplantation or cryopreservation by culturing the cells with Sertoli cells or with Sertoli-cell conditioned media (SCM) prior to transplantation (pre-culturing) or cryopreservation.
摘要翻译：在移植（预培养）或低温保存之前，通过用Sertoli细胞或与支持细胞条件培养基（SCM）培养细胞来增加用于移植或冷冻保存的细胞的活力，数量，存活和成熟的方法。

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