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1. 发明申请

WO2010060218A1 A COMPOSITION FOR USE AS A PEPTIDE RETENTION STANDARD AND A METHOD OF PREDICTING PEPTIDE HYDROPHOBICITY IN LIQUID CHROMATOGRAPHY 审中-公开
标题翻译：用作肽类保留标准的组合物和在液相色谱中预测肽类疏水性的方法
公开(公告)号：WO2010060218A1
公开(公告)日：2010-06-03
申请号：PCT/CA2009/001730
申请日：2009-11-30
申请人： UNIVERSITY OF MANITOBA , KROKHIN, Oleg V. , SPICER, Vic
发明人： KROKHIN, Oleg V. , SPICER, Vic
IPC分类号： G01N30/86 , C40B40/10 , G01N30/02 , C07K7/08 , C07K7/06
CPC分类号： C07K7/06 , G01N30/8668 , G01N30/8672 , G01N2030/042 , G01N2030/8831
摘要： A composition comprising synthetic peptides for use as a peptide retention standard in liquid chromatography applications is disclosed Said synthetic peptides have a broad range of hydrophobicity while maintaining low variation in their molecular weights The synthetic peptides disclosed consist of the ammo acid sequences LGGGGGGDGSR, LGGGGGGDFR, LLGGGGDFG, LLLGGDFR, LLLLDFR, LLLLLDFR A method of predicting the hydrophobicity of an unknown peptide in liquid chromatography using a composition of peptide retention standards and a method of developing a Universal Hydrophobicity Index (UHI) for the measurement of peptide hydrophobicity in liquid chromatography are also described
摘要翻译：公开了包含用于液相色谱应用中的肽保留标准的合成肽的组合物。所述合成肽具有宽范围的疏水性，同时保持其分子量的低变化。所公开的合成肽由氨基酸序列LGGGGGGDGSR，LGGGGGGDFR，LLGGGGDFG LLLGGDFR，LLLLDFR，LLLLLDFR还描述了使用肽保留标准物组合物和用于测量液相色谱中肽疏水性的通用疏水性指数（UHI）的方法在液相色谱中预测未知肽的疏水性的方法

2. 发明申请

WO2011143760A1 METHODS AND SYSTEMS FOR ANALYSIS OF PEPTIDE SAMPLE STREAMS USING TANDEM MASS SPECTROSCOPY 审中-公开
标题翻译：使用TANDEM MASS SPECTROSCOPY分析肽样品流的方法和系统
公开(公告)号：WO2011143760A1
公开(公告)日：2011-11-24
申请号：PCT/CA2011/000586
申请日：2011-05-20
申请人： UNIVERSITY OF MANITOBA , KROKHIN, Oleg V. , SPICER, Vic
发明人： KROKHIN, Oleg V. , SPICER, Vic
IPC分类号： G01N30/72
CPC分类号： H01J49/0036 , G01N30/72 , G01N30/7233 , G01N2030/045 , G01N2030/8831
摘要： The present disclosure relates to methods and systems for analyzing a peptide sample stream from a chromatography column using tandem mass spectroscopy. Analysis of the sample stream during a first time interval is performed in order to identify peptides, such as tryptic peptides, that are contained in the sample stream. Database searching is then performed to identify one or more protein sequences that contain the identified peptide sequence and to identify associated peptide sequences that are contained in the protein sequence that differ from the peptide sequence. The retention time of associated peptides is estimated based on the hydrophobicity of the predicted peptides and by spiking the sample with standard peptides. Information on associated peptides can then be used to configure the mass spectrometer during a second time interval to detect or ignore ions that correspond to the associated peptides.
摘要翻译：本公开涉及用于使用串联质谱从色谱柱分析肽样品流的方法和系统。执行在第一时间间隔期间对样品流的分析，以便鉴定样品流中包含的肽，例如胰蛋白酶肽。然后进行数据库搜索以鉴定含有鉴定的肽序列的一个或多个蛋白质序列，并鉴定与肽序列不同的蛋白质序列中所含的相关肽序列。基于所预测的肽的疏水性并用标准肽掺入样品来估计相关肽的保留时间。然后可以使用关于相关肽的信息在第二时间间隔内配置质谱仪，以检测或忽略与相关肽相对应的离子。

3. 发明申请

WO2008101332A1 TRITICUM MOSAIC VIRUS 审中-公开
公开(公告)号：WO2008101332A1
公开(公告)日：2008-08-28
申请号：PCT/CA2008/000322
申请日：2008-02-20
申请人： UNIVERSITY OF MANITOBA , HABER, Steve , KROKHIN, Oleg , SPICER, Vic , LOVAT, Nicole , YAMCHUK, Andriy , ENS, Verner
发明人： HABER, Steve , KROKHIN, Oleg , SPICER, Vic , LOVAT, Nicole , YAMCHUK, Andriy , ENS, Verner
IPC分类号： C12N15/40 , C07K14/08 , C07K16/10 , C12N15/63 , C12Q1/68 , C12Q1/70
CPC分类号： C12N7/00 , C07K14/005 , C07K16/10 , C12N2770/34022
摘要： In 2006, a mechanically-transmissible, hitherto uncharacterized virus was isolated in Kansas from wheat with mosaic symptoms. Purified preparations contained flexuous, rod-shaped particles (ca 800 x 15nm) that resembled potyviruses The coat protein was estimated from SDS-PAGE to have a mass of ca 35 kDa Its ammo acid sequence, as deduced from DNA sequencing of cloned, reverse-transcribed viral RNA, and separately determined by Time-of-Flight Mass Spectrometry (TOFMS), was most closely related (49% similarity) to Sugarcane streak mosaic virus, a member of the Triimovirus genus of the Potyviriae. The physical and biological evidence supports consideration of this virus as a new potyvirus that naturally infects wheat We suggest the name Triticum mosaic virus (TriMV) Methods are disclosed for the expression and detection of this virus.
摘要翻译： 2006年，在堪萨斯州从具有马赛克症状的小麦中分离出机械传播的迄今为止未表征的病毒。纯化的制剂包含类似于病毒属的弯曲的棒状颗粒（约800×15nm）。从SDS-PAGE估计外壳蛋白具有约35kDa的质量。其氨基酸序列如从克隆的逆转录DNA的测序推导出来的，转录病毒RNA，并且通过飞行时间质谱（TOFMS）单独测定，与甘薯条纹花叶病毒（Potyviriae的Triimovirus属的成员）最密切相关（49％相似）。物理和生物学证据支持将该病毒考虑为天然感染小麦的新型病毒。我们建议公告小麦花叶病毒（TriMV）的名称，用于表达和检测该病毒。

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