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1. 发明申请

WO2012013337A1 N-GLYCOSYLATION IN TRANFORMED PHAEODACTYLUM TRICORNUTUM 审中-公开
标题翻译：经改造的PHAEODACTYLUM TRICORNUTUM中的N-糖苷化
公开(公告)号：WO2012013337A1
公开(公告)日：2012-02-02
申请号：PCT/EP2011/003756
申请日：2011-07-27
申请人： UNIVERSITE DE ROUEN , L'INSTITUT FRANCAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER (IFREMER) , ALGENICS , BARDOR, Muriel , LOUVET, Romain , SAINT-JEAN, Bruno , BUREL, Carole , BAIET, Bérangère , CADORET, Jean-Paul , LEROUGE, Patrice , MICHEL, Rémy
发明人： BARDOR, Muriel , LOUVET, Romain , SAINT-JEAN, Bruno , BUREL, Carole , BAIET, Bérangère , CADORET, Jean-Paul , LEROUGE, Patrice , MICHEL, Rémy
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/13 , C07K14/505
CPC分类号： C12N15/8243 , C07K14/505 , C12N9/1051 , C12N9/2402 , C12P21/005 , C12Y302/01096 , Y02P20/52
摘要： The present invention concerns transformed Phaeodactylum tricomutum comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an a-Mannosidase II and wherein at least one β-Ν-acetylglucosaminidase of said transformed Phaeodactylum tricomutum has been inactivated; a method for producing a glycosylated polypeptide, said method comprising the steps of (i) culturing a transformed P. tricomutum as defined previously and (ii) purifying said polypeptide that is expressed and glycosylated in said transformed P. tricomutum; and the use of such a transformed P. tricomutum for producing a glycosylated polypeptide.
摘要翻译：本发明涉及包含与启动子可操作地连接的核酸序列的转化的毛果乳杆菌，其中所述核酸序列编码N-乙酰氨基葡糖酰转移酶I和/或α-甘露糖苷酶II，并且其中所述转化的至少一种β-β-乙酰氨基葡糖苷酶 Phaeodactylum tricomutum已灭活; 制备糖基化多肽的方法，所述方法包括以下步骤：（i）培养如前所定义的转化的毛细管毛细管管，和（ii）纯化在所述转化的毛细管毛细管中表达和糖基化的所述多肽; 以及使用这种经转化的毛细管曲巴生产糖基化多肽的方法。

2. 发明申请

WO2009101160A1 PRODUCTION OF GLYCOSYLATED POLYPEPTIDES IN MICROALGAE 审中-公开
标题翻译：在微藻中生产谷氨酰胺多糖
公开(公告)号：WO2009101160A1
公开(公告)日：2009-08-20
申请号：PCT/EP2009/051672
申请日：2009-02-12
申请人： INSTITUT FRANCAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER (IFREMER) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - CNRS - , UNIVERSITE DE ROUEN , CADORET, Jean-Paul , CARLIER, Aude , LEROUGE, Patrice , BARDOR, Muriel , BUREL, Carole , MAURY, Florian
发明人： CADORET, Jean-Paul , CARLIER, Aude , LEROUGE, Patrice , BARDOR, Muriel , BUREL, Carole , MAURY, Florian
IPC分类号： C12N1/12 , C12N15/82
CPC分类号： C12P21/005 , C07K14/4756 , C07K14/505 , C12N1/12 , C12N9/1051 , C12N9/2402 , C12N15/79 , C12N15/8257 , C12Y302/01045 , C12Y302/01096 , C12Y305/01052
摘要： The invention relates to transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.
摘要翻译：本发明涉及能够表达糖基化多肽的转化微藻和用于产生所述转化的微藻并产生糖基化多肽的方法。

3. 发明申请

WO2012089342A1 PRODUCTION OF HIGH MANNOSE GLYCOSYLATED PROTEINS STORED IN THE PLASTID OF MICROALGAE 审中-公开
标题翻译：生产存在于微藻中的高甘露糖苷化蛋白
公开(公告)号：WO2012089342A1
公开(公告)日：2012-07-05
申请号：PCT/EP2011/006592
申请日：2011-12-28
申请人： ALGENICS , CARLIER, Aude , MICHEL, Rémy , CADORET, Jean-Paul , LEJEUNE, Alexandre , DUFOURMANTEL, Nathalie
发明人： CARLIER, Aude , MICHEL, Rémy , CADORET, Jean-Paul , LEJEUNE, Alexandre , DUFOURMANTEL, Nathalie
IPC分类号： C12N15/82 , C12P21/00 , C12R1/89
CPC分类号： C07K14/005 , C07K14/505 , C12N9/2402 , C12N15/8214 , C12N15/8257 , C12N15/8258 , C12N2740/16122 , C12P21/005 , C12Q1/34 , C12Y302/01045 , G01N33/6842
摘要： The present invention concerns a transformed microalga producing a protein harboring a "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of said protein, 3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated.
摘要翻译：本发明涉及转化的微藻，其在所述转化的微藻的质体中产生具有糖基化的“高甘露糖”模式的蛋白质，其中1）所述转化的微藻具有叶绿体内质网（CER）; 2）所述微藻已经用可操作地连接到启动子的核酸序列转化，所述核酸序列编码氨基酸序列，其包含（i）至少由信号肽组成的氨基末端二分基因信号（BTS）序列，通过转运肽; 和（ii）所述蛋白质的序列，3）所述微藻的木糖基转移酶和岩藻糖基转移酶尚未失活; 4）所述微藻的N-乙酰基糖基转移酶I未被灭活，优选所述微藻的N-乙酰基糖基转移酶II，III，IV，V和VI，甘露糖苷酶II和糖基转移酶未被灭活。

4. 发明申请

WO2012052170A1 SECRETION OF RECOMBINANT POLYPEPTIDES IN THE EXTRACELLULAR MEDIUM OF DIATOMS 审中-公开
标题翻译：重组多糖在细胞外细胞培养中的分泌
公开(公告)号：WO2012052170A1
公开(公告)日：2012-04-26
申请号：PCT/EP2011/005282
申请日：2011-10-20
申请人： ALGENICS , LEJEUNE, Alexandre , MICHEL, Rémy , CADORET, Jean-Paul , CARLIER, Aude
发明人： LEJEUNE, Alexandre , MICHEL, Rémy , CADORET, Jean-Paul , CARLIER, Aude
IPC分类号： C12N15/82 , C12P21/02 , C07K14/505 , C07K14/55
CPC分类号： C12P21/00 , C07K14/505 , C07K14/55 , C12N15/8257 , C12P21/02
摘要： The present invention concerns a transformed diatom comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide and (ii) a polypeptide, said heterologous signal peptide leading to the secretion of said polypeptide in the extracellular medium of said transformed diatom; a method for producing a polypeptide which is secreted in the extracellular medium, said method comprising the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of said culture and (iii) purifying the secreted polypeptide in said extracellular medium; and use of said transformed diatom for the secretion of a polypeptide in the extracellular medium.
摘要翻译：本发明涉及包含可操作地连接到启动子的核酸序列的转化的硅藻，其中所述核酸序列编码包含（i）异源信号肽和（ii）多肽的氨基酸序列，所述异源信号肽导致在所述转化的硅藻的细胞外培养基中分泌所述多肽; 一种产生分泌在细胞外介质中的多肽的方法，所述方法包括以下步骤：（i）培养转化的硅藻，（ii）收获所述培养物的细胞外培养物，和（iii）纯化所述细胞外培养基中分泌的多肽 ; 以及使用所述转化的硅藻在细胞外培养基中分泌多肽。

5. 发明申请

WO2013023786A1 NOVEL ISOCHRYSIS SP TAHITIAN CLONE AND USES THEREFORE 审中-公开
标题翻译： NOVEL ISOCHRYSIS SP TAITIAN克隆及其用途
公开(公告)号：WO2013023786A1
公开(公告)日：2013-02-21
申请号：PCT/EP2012/003491
申请日：2012-08-16
申请人： INSTITUT FRANCAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER (IFREMER) , DUBOIS, Nolween , CADORET, Jean-Paul , BOUGARAN, Gaël , ROUXEL, Catherine , DOULIN, Sophie
发明人： DUBOIS, Nolween , CADORET, Jean-Paul , BOUGARAN, Gaël , ROUXEL, Catherine , DOULIN, Sophie
IPC分类号： C12N1/12 , C12R1/89 , C12P7/64
CPC分类号： A61K8/37 , A23L33/115 , A61K8/361 , A61K8/922 , A61K2800/92 , A61Q19/00 , A61Q19/007 , A61Q19/02 , A61Q19/08 , C10L1/02 , C12N1/12 , C12R1/89
摘要： The present invention relates to a novel microalgal strain whose lipid production has been enhanced compared to the wild type. Said lipid content produced by the microalgae of the invention is particularly rich in neutral lipids. The present invention also relates to the use of said microalgal strain and said lipids.
摘要翻译：本发明涉及一种与野生型相比其脂质产生增强的新型微藻菌株。由本发明的微藻产生的所述脂质含量特别富含中性脂质。本发明还涉及所述微藻类和所述脂质的用途。

6. 发明申请

WO2010084274A1 PROCEDE DE FIXATION DE CO2 ET DE TRAITEMENT DE DECHETS ORGANIQUES PAR COUPLAGE D'UN SYSTEME DE DIGESTION ANAEROBIE ET D'UN SYSTEME DE PRODUCTION DE MICROORGANISMES PHYTOPLANCTONIQUES 审中-公开
标题翻译：用于固定二氧化碳和通过耦合无机废物系统和PHYTOPLANKTON微生物生产系统处理有机废物的方法
公开(公告)号：WO2010084274A1
公开(公告)日：2010-07-29
申请号：PCT/FR2010/050008
申请日：2010-01-05
申请人： INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE - INRA , INSTITUT NATIONAL DE RECHERCHE EN INFORMATIQUE ET EN AUTOMATIQUE - INRIA , INSTITUT FRANCAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER - IFREMER , BERNARD, Olivier , BOUGARAN, Gael , CADORET, Jean-Paul , KAAS, Raymond , LATRILLE, Eric , SIALVE, Bruno , STEYER, Jean-Philippe
发明人： BERNARD, Olivier , BOUGARAN, Gael , CADORET, Jean-Paul , KAAS, Raymond , LATRILLE, Eric , SIALVE, Bruno , STEYER, Jean-Philippe
IPC分类号： B01D53/62 , B01D53/84 , C02F3/28 , C02F11/04 , C12P5/02
CPC分类号： C02F3/327 , B01D53/62 , B01D53/84 , B01D2251/95 , B01D2256/24 , B01D2257/504 , C02F11/04 , C02F2209/06 , C02F2209/14 , C12M21/02 , C12M21/04 , C12M23/18 , Y02A50/2358 , Y02C10/02 , Y02C10/04 , Y02E50/343 , Y02P20/59 , Y02W10/18 , Y02W10/23 , Y02W10/37
摘要： L'invention concerne un procédé de fixation de CO2 et de traitement de déchets organiques, dans lequel on traite des microorganismes (105) issus d'une culture phytoplanctonique et des déchets organiques (104) dans un réacteur d'hydrolyse (101); on traite au moins une partie d'un effluent liquide (109) sortant du réacteur d'hydrolyse dans un réacteur de méthanisation (102); on traite une phase liquide (127) et du biogaz à épurer (110) sortant de l'étape (a") dans une unité de culture de microorganismes phytoplanctoniques (103); on injecte un effluent gazeux (113) contenant du CO 2 dans l'unité de culture de microorganismes phytoplanctoniques; on maintient une concentration en NH 3 inférieure à 0,5 g/L dans le réacteur de méthanisation; et on récupère un biogaz enrichi en méthane au sortir de l'unité de culture de microorganismes phytoplanctoniques. L'invention concerne également un système pour mettre en œuvre ce procédé.
摘要翻译：本发明涉及一种用于固定CO 2和用于处理有机废物的方法，其包括：在水解反应器（101）中处理来自浮游植物培养物的微生物（105）和有机废物（104）; 处理至少一个在甲烷化反应器（102）中离开水解反应器的液体流出物（109）的部分; 在浮游植物微生物培养单元（103）中处理液相（127）和待清洁的生物气体（110）并由步骤（a“）产生; 将含CO 2的气态流出物注入到浮游植物微生物培养单元中; 在甲烷化反应器中保持NH 3浓度低于0.5g / L; 并在浮游植物微生物培养单元出口处回收甲烷富集沼气。本发明还涉及一种用于实现所述方法的系统。

7. 发明申请

WO2013164095A1 PRODUCTION OF SECRETED THERAPEUTIC ANTIBODIES IN MICROALGAE 审中-公开
标题翻译：微生物中分泌的治疗性抗体的生产
公开(公告)号：WO2013164095A1
公开(公告)日：2013-11-07
申请号：PCT/EP2013/001302
申请日：2013-05-02
申请人： ALGENICS
发明人： CARLIER, Aude , MICHEL, Rémy , LEJEUNE, Alexandre , CADORET, Jean-Paul
IPC分类号： C12N15/82
CPC分类号： C07K16/2887 , C07K16/2863 , C07K2317/13 , C07K2317/24 , C07K2317/732 , C07K2317/76 , C12N1/12 , C12N15/79 , C12N15/8258
摘要： The invention relates to a transformed microalga comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, said transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and said microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, said therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content. The present invention also relates to a method for producing said therapeutic antibody, a functional fragment or a derivative thereof, to the use of a transformed microalga according to the invention for the production and the secretion of a therapeutic antibody, a functional fragment or a derivative thereof in the extracellular medium, to a therapeutic antibody, a functional fragment or a derivative thereof produced and secreted in the extracellular medium of microalgae by a method according to the invention and to a pharmaceutical composition comprising said therapeutic antibody, functional fragment or derivative thereof.
摘要翻译：本发明涉及包含可操作地连接到启动子的核酸序列的转化微藻，其中所述核酸序列编码包含（i）异源信号肽的氨基酸序列; 和（ii）治疗性抗体，功能片段或其衍生物，所述转化微藻表达分泌在细胞外介质中的治疗性抗体，功能片段或衍生物，所述微藻选自除Volvocales之外的绿藻，以及红藻，铬酸盐和黑醋酸盐。优选地，所述治疗性抗体，其功能片段或衍生物具有增加的抗体依赖性细胞介导的细胞毒性（ADCC）和低岩藻糖含量。本发明还涉及使用根据本发明的转化微藻用于产生和分泌治疗性抗体，功能片段或衍生物的所述治疗性抗体，功能片段或其衍生物的制备方法通过本发明的方法产生和分泌在微藻细胞外培养基中的治疗性抗体，功能片段或衍生物，以及包含所述治疗性抗体，功能片段或衍生物的药物组合物。

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