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    • 3. 发明申请
    • METHODS FOR ELECTRICALLY DETECTING INTERACTIONS BETWEEN BIOMOLECULES USING SCANNING TUNNELING MICROSCOPE
    • 用扫描隧道显微镜电检测生物分子间相互作用的方法
    • WO2009093813A2
    • 2009-07-30
    • PCT/KR2008007655
    • 2008-12-24
    • UNIV SOGANG IND UNIV COOP FOUNCHOI JEONG-WOOOH BYUNG-KEUNLEE WOOCHANGKANG DA-YEON
    • CHOI JEONG-WOOOH BYUNG-KEUNLEE WOOCHANGKANG DA-YEON
    • G01Q60/14
    • G01Q60/14B82Y35/00G01N33/54346G01N33/5438G01N33/553G01N33/585
    • The present invention relates to a method for electrically detecting an interaction between biomolecules using a scanning tunneling microscope (STM), comprising the steps of: (a) immobilizing a first biomolecule on a metal substrate; (b) applying a second biomolecule to the metal substrate to induce the interaction between the first biomolecule and the second biomolecule; and (c) scanning the metal substrate using STM to measure an electric signal, whereby the occurrence of the interaction between the first and the second biomolecules is determined. The present STM-based electrical detection method enables to detect analytes having much lower concentrations {e.g., 100 fg/mL). In addition, the method of the present invention allows multiple detections in a single measurement system {e.g., single protein chip). On the other hand, the present invention permits to successfully analyze a nano-size array of which detection is impossible in a conventional method based on the fluorescence and allows ultrahigh sensitivity by signal enhancement through silver staining of Au nanoparticles.
    • 本发明涉及一种使用扫描隧道显微镜(STM)电检测生物分子之间的相互作用的方法,该方法包括以下步骤:(a)将第一生物分子固定在金属基底上; (b)将第二生物分子施加至金属基材以诱导第一生物分子与第二生物分子之间的相互作用; 和(c)使用STM扫描金属基底以测量电信号,由此确定第一生物分子和第二生物分子之间的相互作用的发生。 本发明的基于STM的电检测方法能够检测具有低得多浓度(例如100fg / mL)的分析物。 另外,本发明的方法允许在单个测量系统中进行多次检测(例如,单个蛋白质芯片)。 另一方面,本发明允许成功地分析在基于荧光的常规方法中不可能检测到的纳米尺寸阵列,并且允许通过金纳米颗粒的银染的信号增强来实现超高灵敏度。