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1. 发明申请

WO2013009679A2 OVER-EXPRESSION OF A PUTATIVE OXIDOREDUCTASE (UCPA) FOR INCREASING FURFURAL OR 5-HYDROXYMETHYLFURFURAL TOLERANCE 审中-公开
标题翻译：用于增加糠醛或5-羟甲基糠醛耐受性的对照性氧化还原酶（UCPA）的过度表达
公开(公告)号：WO2013009679A2
公开(公告)日：2013-01-17
申请号：PCT/US2012045912
申请日：2012-07-09
申请人： UNIV FLORIDA , WANG XUAN , MILLER ELLIOT N , YOMANO LORRAINE P , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O'NEAL
发明人： WANG XUAN , MILLER ELLIOT N , YOMANO LORRAINE P , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O'NEAL
IPC分类号： C12N1/21 , C12N1/15 , C12N1/19 , C12N15/53 , C12P7/04 , C12P7/40 , C12P13/04
CPC分类号： C12P13/001 , C07K14/245 , C12N9/0004 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/40 , C12P7/44 , C12P7/46 , C12P7/54 , C12P7/56 , C12P2203/00 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.
摘要翻译：本发明涉及用于增加遗传修饰微生物中糠醛耐受性的推定氧化还原酶（ucpA）的过表达。还提供了能够过表达UcpA的基因修饰的微生物。显示ucpA的表达增加使糠醛耐受性增加50％，并且允许在存在15mM糠醛的情况下将糖发酵成产物。

2. 发明申请

WO2012135420A3 OVER-EXPRESSION OF HADH-DEPENDENT OXIDOREDUCTASE (FUCO) FOR INCREASING FURFURAL OR 5-HYDROXYMETHYLFURFURAL TOLERANCE 审中-公开
标题翻译：依赖HADH的氧化还原酶（FUCO）增加镰刀形或5-羟甲基糠醛耐受性的过度表达
公开(公告)号：WO2012135420A3
公开(公告)日：2012-12-27
申请号：PCT/US2012031082
申请日：2012-03-29
申请人： UNIV FLORIDA , MILLER ELLIOT N , ZHANG XUELI , YOMANO LORRAINE P , WANG XUAN , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O'NEAL
发明人： MILLER ELLIOT N , ZHANG XUELI , YOMANO LORRAINE P , WANG XUAN , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O'NEAL
IPC分类号： C12N15/53 , C12N15/63 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/40
CPC分类号： C12P7/56 , C12N9/0006 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/46 , C12Y101/01202 , Y02E50/16 , Y02E50/17
摘要： The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.
摘要翻译：本发明涉及NADH依赖性丙二醇氧化还原酶（FucO）可以减少糠醛的发现。这允许采用新方法来提高用于生产期望产品的细菌和/或酵母细胞中的糠醛耐受性。因此，提供了对糠醛和5-羟甲基糠醛（5-HMF）具有增加的耐受性的新型生物催化剂（细菌，真菌或酵母细胞），以及制备和使用这种生物催化剂用于生产期望产物的方法。

3. 发明申请

WO2012135420A8 OVER-EXPRESSION OF HADH-DEPENDENT OXIDOREDUCTASE (FUCO) FOR INCREASING FURFURAL OR 5-HYDROXYMETHYLFURFURAL TOLERANCE 审中-公开
标题翻译：用于增加糠醛或5-羟基甲基葡萄胎耐受性的依赖于依赖于氧化还原酶（FUCO）的过度表达
公开(公告)号：WO2012135420A8
公开(公告)日：2012-11-08
申请号：PCT/US2012031082
申请日：2012-03-29
申请人： UNIV FLORIDA , MILLER ELLIOT N , ZHANG XUELI , YOMANO LORRAINE P , WANG XUAN , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O'NEAL
发明人： MILLER ELLIOT N , ZHANG XUELI , YOMANO LORRAINE P , WANG XUAN , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O'NEAL
IPC分类号： C12N15/53 , C12N15/63 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/40
CPC分类号： C12P7/56 , C12N9/0006 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/46 , C12Y101/01202 , Y02E50/16 , Y02E50/17
摘要： The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.
摘要翻译：本发明涉及NADH依赖性丙二醇氧化还原酶（FucO）可以减少糠醛的发现。这允许用于改善用于产生所需产物的细菌和/或酵母细胞中的糠醛耐受性的新方法。因此，提供了对糠醛和5-羟甲基糠醛（5-HMF）具有增加的耐受性的新型生物催化剂（细菌，真菌或酵母细胞），其是制备和使用这些生物催化剂以制备所需产物的方法。

4. 发明申请

WO2007120198A3 MATERIALS AND METHODS FOR IMPROVED MICROBIAL PRODUCTION OF ORGANIC COMPOUNDS 审中-公开
标题翻译：改进微生物生产有机化合物的材料和方法
公开(公告)号：WO2007120198A3
公开(公告)日：2007-12-21
申请号：PCT/US2006043380
申请日：2006-11-07
申请人： UNIV FLORIDA , INGRAM LONNIE O'NEAL , SHANMUGAM KEELNATHAM T , ZHOU SHENGDE , CAUSEY THOMAS B , YOMANO LORRAINE P , GRABAR TAMMY B
发明人： INGRAM LONNIE O'NEAL , SHANMUGAM KEELNATHAM T , ZHOU SHENGDE , CAUSEY THOMAS B , YOMANO LORRAINE P , GRABAR TAMMY B
IPC分类号： C12P1/00 , C12N1/21
CPC分类号： C12P7/56 , C12P7/40
摘要： In accordance with the subject invention, osmoprotectants, such as betaine, may be used to increase the productivity and titer of anaerobic and aerobic fermentation with E. coli. and other microbes. Betaine serves as a protective osmolyte during fermentations with high concentrations of sugars or products, replacing the need to divert carbon and energy into native osmoprotectants such as glutamate and trehalose. Thus, in accordance with the subject invention, betaine and/or other osmoprotectants can be added to media and improve the rates of biological conversion of sugars into chemicals such as organic acids.
摘要翻译：根据本发明，可以使用渗透保护剂（例如甜菜碱）来提高用大肠杆菌进行厌氧和需氧发酵的生产率和效价。和其他微生物。甜菜碱在用高浓度的糖或产品发酵时用作保护性渗透液，代替将碳和能量转移到天然渗透保护剂如谷氨酸和海藻糖的需要。因此，根据本发明，可以将甜菜碱和/或其它渗透保护剂加入到培养基中并提高糖类生物转化为化学物质如有机酸的速率。

5. 发明申请

WO2008021141A2 RE-ENGINEERING BACTERIA FOR ETHANOL PRODUCTION 审中-公开
标题翻译：用于乙醇生产的重新工程细菌
公开(公告)号：WO2008021141A2
公开(公告)日：2008-02-21
申请号：PCT/US2007017646
申请日：2007-08-08
申请人： UNIV FLORIDA , YOMANO LORRAINE P , YORK SEAN W , ZHOU SHENGDE , SHANMUGAM KEELNATHAM , INGRAM LONNIE O
发明人： YOMANO LORRAINE P , YORK SEAN W , ZHOU SHENGDE , SHANMUGAM KEELNATHAM , INGRAM LONNIE O
IPC分类号： C12N1/21
CPC分类号： C12P7/065 , Y02E50/17
摘要： The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.
摘要翻译：本发明提供重组细菌，其包含全部异源乙醇生产基因。异源乙醇生产基因的完整补体的表达导致重组细菌在无矿物质培养基中生长时产生乙醇作为主要的发酵产物，而不添加复杂的营养物质。还公开了使用重组细菌生产重组细菌的方法和生产乙醇的方法。

6. 发明申请

WO2007005646A3 RECOMBINANT HOST CELLS AND MEDIA FOR ETHANOL PRODUCTION 审中-公开
标题翻译：用于乙醇生产的重组宿主细胞和培养基
公开(公告)号：WO2007005646A3
公开(公告)日：2007-08-02
申请号：PCT/US2006025655
申请日：2006-06-30
申请人： UNIV FLORIDA , WOOD BRENT E , INGRAM LONNIE O , YOMANO LORRAINE P , YORK SEAN W
发明人： WOOD BRENT E , INGRAM LONNIE O , YOMANO LORRAINE P , YORK SEAN W
IPC分类号： C12N1/19
CPC分类号： C12N1/22 , C12P7/065 , Y02E50/17
摘要： Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.
摘要翻译：公开了适于降解已经针对高产量乙醇的生长和产生进行了优化的寡糖的重组宿主细胞以及制备和使用这些细胞的方法。本发明进一步提供了包含尿素样化合物的基本培养基，用于通过重组微生物经济地生产乙醇。根据本发明的重组宿主细胞通过基因突变进行修饰以消除负责产生除乙醇以外的不需要的产物的基因，由此相对于未突变的亲本菌株增加了由寡糖产生的乙醇的产量。当在适于在含有廉价试剂的最小生长培养基中发酵的条件下生长时，新的改进的重组菌株能够具有优异的乙醇生产力和产量。针对乙醇生产优化的系统将选定的优化基本培养基与优化用于所选培养基的重组宿主细胞结合。优选的系统适合于通过使用木质纤维素作为寡糖来源的同时糖化和发酵（SSF）进行有效的乙醇生产。本发明还提供了新的分离的多核苷酸序列，多肽序列，载体和抗体。

7. 发明申请

WO2007005646A9 RECOMBINANT HOST CELLS AND MEDIA FOR ETHANOL PRODUCTION 审中-公开
公开(公告)号：WO2007005646A9
公开(公告)日：2007-03-22
申请号：PCT/US2006025655
申请日：2006-06-30
申请人： UNIV FLORIDA , WOOD BRENT E , INGRAM LONNIE O , YOMANO LORRAINE P , YORK SEAN W
发明人： WOOD BRENT E , INGRAM LONNIE O , YOMANO LORRAINE P , YORK SEAN W
IPC分类号： C12P7/06 , C12N1/18
CPC分类号： C12N1/22 , C12P7/065 , Y02E50/17
摘要： Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.

8. 发明申请

WO2015095298A3 USE OF POLYAMINES AND POLYAMINE TRANSPORTERS TO PROVIDE FURFURAL TOLERANCE 审中-公开
标题翻译：使用聚胺和多胺转运蛋白来提供镰刀的耐受性
公开(公告)号：WO2015095298A3
公开(公告)日：2015-11-12
申请号：PCT/US2014070795
申请日：2014-12-17
申请人： UNIV FLORIDA
发明人： GEDDES RYAN D , WANG XUAN , YOMANO LORRAINE P , MILLER ELLIOT N , ZHENG HUABO , SHANMUGAM KEELNATHAM T , INGRAM LONNIE O
IPC分类号： C12N1/21 , C12N1/20 , C12P7/06
CPC分类号： C12P7/10 , C07K14/195 , C07K14/245 , C07K14/37 , C07K14/39 , C12N1/22 , C12P7/065 , C12P2203/00 , Y02E50/16 , Y02E50/17
摘要： The subject invention pertains to microorganisms comprising genetic modifications involving one or more target genes encoding: putrescine-ornithine antiporter (PotE), putrescine importer (PuuP), ATP-dependent polyamine transporter (PotABCD), and low affinity putrescine-importer (PlaP), wherein the genetic modifications cause increased expression and/or activity of the polypeptides produced by the target genes. The methods for producing a desired product by fermenting a biomass in the presence of the microorganism of the current invention are also provided. Additionally, the current invention pertains to methods of increasing furfural and/or 5-hydroxymethylfurfural (5-HMF) resistance in a microorganism by introducing in to the microorganism genetic modifications involving one or more target genes encoding: PotE, PuuP, PotABCD, and PlaP, wherein the genetic modifications cause increased expression and/or activity of the polypeptides produced by said target genes and/or culturing the microorganism in the presence of one or more polyamines.
摘要翻译：本发明涉及包含编码以下一种或多种靶基因的遗传修饰的微生物：腐胺 - 鸟氨酸反向转运蛋白（PotE），腐胺进口物（PuuP），ATP-依赖性多胺转运蛋白（PotABCD）和低亲和力腐胺 - 进口物（PlaP）其中遗传修饰引起由靶基因产生的多肽的表达和/或活性增加。还提供了通过在本发明的微生物存在下发酵生物质来生产期望产物的方法。另外，本发明涉及通过向微生物中引入涉及编码以下的一种或多种靶基因的基因修饰来增加微生物中糠醛和/或5-羟甲基糠醛（5-HMF）抗性的方法：PotE，PuuP，PotABCD和PlaP 其中所述遗传修饰引起由所述靶基因产生的多肽的表达和/或活性增加和/或在一种或多种多胺存在下培养所述微生物。

9. 发明申请

WO9845425A9 DEVELOPMENT OF HIGH-ETHANOL RESISTANT ESCHERICHIA COLI 审中-公开
标题翻译：高分子抗性食品科学研究进展
公开(公告)号：WO9845425A9
公开(公告)日：1999-11-04
申请号：PCT/US9806405
申请日：1998-03-31
申请人： UNIV FLORIDA
发明人： INGRAM LONNIE O , YOMANO LORRAINE P , YORK SEAN W
IPC分类号： C12N15/09 , C12N1/21 , C12N1/36 , C12P7/06 , C12R1/18 , C12R1/19 , C12R1/22 , C12R1/64 , C12N15/01
CPC分类号： C12N1/36 , C12P7/065 , C12R1/19 , Y02E50/17
摘要： The invention relates to a novel selection process to identify novel mutants of Escherichia coli KO11, an ethanologenic bacterium, that exhibit the ability to grow and survive in ethanol concentrations beyond that in which the parent Escherichia coli KO11 can survive. The new approach, which alternates between selection for ethanol resistance and selection for rapid growth on solid medium containing a high level of chloramphenicol, resulted in strains which are potentially useful for ethanol production.
摘要翻译：本发明涉及鉴定大肠杆菌KO11（一种产乙醇细菌）的新型突变体的新型选择方法，它表现出在乙醇浓度以外生长和存活的能力，超过了亲本大肠杆菌KO11可以存活的突变体。在高含量氯霉素的固体培养基上，乙醇抗性选择和快速生长选择之间的新方法交替出现，这些菌株对乙醇生产具有潜在的应用价值。

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