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1. 发明申请

WO2013004852A3 MAGNETIC BEAD AGGREGATION ASSAY SYSTEM AND METHOD FOR ANALYSING AND CHARACTERISING MAGNETIC BEAD AGGREGATION AND DETECTION OF TARGET ANALYTES 审中-公开
标题翻译：用于分析和表征磁珠聚集的磁珠分离测定系统和方法和目标分析仪的检测
公开(公告)号：WO2013004852A3
公开(公告)日：2014-11-06
申请号：PCT/EP2012063440
申请日：2012-07-09
申请人： UNIV DUBLIN , LEE GIL , LI PENG , PLATT MARK , CANNON GEMMA
发明人： LEE GIL , LI PENG , PLATT MARK , CANNON GEMMA
IPC分类号： B03C1/032 , B03C1/24 , B03C1/28 , G01N27/74 , G01N33/543
CPC分类号： G01N33/54306 , B03C1/032 , B03C1/24 , B03C1/288 , B03C2201/18 , B03C2201/24 , B03C2201/26 , G01N27/745 , G01N33/54326 , G01N33/54346
摘要： There is provided a method of aggregating a plurality of beads in a magnetic bead aggregation assay for subsequent analysis comprising: - providing magnetic beads comprising a capture probe for binding with said target analyte; - reacting the magnetic beads with the sample including a target analyte in a reaction chamber aggregating the magnetic beads in the presence of a magnetic field with the target analyte to allow formation of magnetic bead aggregates having physical properties detectable to enable characterisation of the aggregates on an aggregate by aggregate basis using a detector to measure the physical properties of the aggregates. Further provided are a method and system for detecting analytes in a sample by characterising the magnetic bead aggregates on an aggregate by aggregate basis by measuring physical properties of the aggregates.
摘要翻译：提供了一种在磁珠聚集测定中聚集多个珠子以进行后续分析的方法，包括：提供包含用于与所述目标分析物结合的捕获探针的磁珠; - 使磁珠与包含靶分析物的样品在反应室中反应，所述反应室在磁场存在下与目标分析物聚集磁珠，以允许形成具有可检测的物理性质的磁珠聚集体，以使得能够在使用检测器以聚集为基础的聚集体来测量聚集体的物理性质。进一步提供了一种通过测量聚集体的物理性质通过聚集体来表征聚集体上的磁珠聚集体来检测样品中的分析物的方法和系统。

2. 发明申请

WO2012004363A2 NON-LINEAR MAGNETOPHORETIC SEPARATION DEVICE, SYSTEM AND METHOD 审中-公开
标题翻译：非线性磁分离装置，系统和方法
公开(公告)号：WO2012004363A2
公开(公告)日：2012-01-12
申请号：PCT/EP2011061550
申请日：2011-07-07
申请人： UNIV DUBLIN , LEE GIL , LI PENG , PLATT MARK , CANNON GEMMA
发明人： LEE GIL , LI PENG , PLATT MARK , CANNON GEMMA
IPC分类号： B03C1/032
CPC分类号： B03C1/02 , B03C1/032 , B03C1/24 , B03C1/288 , B03C2201/18 , B03C2201/24 , B03C2201/26 , G01N27/44756
摘要： A flow enhanced method and system for flow non-linear magnetophoresis (F-NLM) is described. By tuning an external field frequency and the flow rate the migration velocities of different bead types may be caused to differ by several orders of magnitude over an extended range of frequencies to allow for separation of particles. Use of such efficiency in separation in bio-separation and similar assays is described.
摘要翻译：描述了用于流动非线性磁电泳（F-NLM）的流动增强方法和系统。通过调整外场频率和流速，可以使不同珠粒类型的迁移速度在扩展的频率范围内相差几个数量级以允许分离颗粒。描述了在生物分离中的这种效率的使用和类似的测定。

3. 发明申请

WO2013087785A2 BOWMAN-BIRK PROTEASE INHIBITOR (BBI) BINDING PEPTIDES, AND USES THEREOF IN THE SEPARATION, DETECTION OR PURIFICATION OF BBI 审中-公开
标题翻译： BOWMAN-BIRK蛋白酶抑制剂（BBI）结合肽及其在分离中的用途，BBI的检测或纯化
公开(公告)号：WO2013087785A2
公开(公告)日：2013-06-20
申请号：PCT/EP2012075417
申请日：2012-12-13
申请人： UNIV DUBLIN
发明人： LEE GIL , MUZARD JULIEN , FIELDS CONOR
IPC分类号： C07K14/81 , C07K7/08 , C07K16/38
CPC分类号： C07K14/811 , C07K16/2803 , C07K16/38 , C07K2319/00
摘要： Peptide probes against the soybean Bowman-Birk Inhibitor (BBI) for use in the detection, separation, or purification of BBI from complex protein mixtures such as soy whey, and as an affinity separation matrix comprising a peptide probe of the invention immobilised to a support such as a magnetic bead, are described.
摘要翻译：针对大豆Bowman-Birk抑制剂（BBI）的肽探针用于从复杂蛋白质混合物如大豆乳清中检测，分离或纯化BBI，并且作为包含固定在载体上的本发明的肽探针的亲和分离基质例如磁珠。

4. 发明申请

WO2013178802A3 MICROPARTICLES AND A SYSTEM AND METHOD FOR THE SYNTHESIS OF MICROPARTICLES 审中-公开
标题翻译：微量元素和微生物合成的系统和方法
公开(公告)号：WO2013178802A3
公开(公告)日：2014-02-27
申请号：PCT/EP2013061302
申请日：2013-05-31
申请人： UNIV DUBLIN
发明人： LEE GIL , O'MAHONY JAMES , PLATT MARK
IPC分类号： A61K9/50 , B01J13/04
CPC分类号： C01G49/02 , A61B5/055 , A61K9/5094 , B01F3/0811 , B01F3/0853 , B01F3/223 , B01F7/00 , B01F15/0203 , B01F2015/062 , B01J13/043 , B01J13/125 , C01P2004/61 , C01P2006/42 , H01F1/0054
摘要： There is provided a method of producing microparticles using an emulsion based synthesis route including: Providing a first fluid phase and a second fluid phase, wherein the first fluid phase is a continuous phase and the second fluid phase is a dispersed phase comprising a dispersed material, wherein the continuous phase is immiscible with the dispersed phase; Mixing the first continuous phase and the second dispersed phase in the presence of a surfactant in a shear device to form an emulsion of droplets of controllable size and having a narrow drop size distribution; Drying the emulsion to form microparticles of controllable size and having narrow size distribution, and wherein the microparticles may comprise spherical, crumpled, dimpled, porous or hollow microparticles morphology. Also provided is a system including shear device and drying arrangement. Also provided are microparticles of controllable size and morphology formed by the method.
摘要翻译：提供了使用基于乳液的合成途径制备微粒的方法，包括：提供第一流体相和第二流体相，其中第一流体相是连续相，第二流体相是包含分散材料的分散相，其中连续相与分散相不混溶; 在剪切装置中在存在表面活性剂的情况下将第一连续相和第二分散相混合以形成可控尺寸并具有窄液滴尺寸分布的液滴的乳液; 干燥乳液以形成可控尺寸并且具有窄尺寸分布的微粒，并且其中微粒可以包含球形，皱缩的，凹陷的，多孔的或中空的微粒形态。还提供了包括剪切装置和干燥装置的系统。还提供了通过该方法形成的可控尺寸和形态的微粒。

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