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1. 发明申请

WO2010096720A3 METHODS AND COMPOSITIONS FOR ALTERING PHOTOPHYSICAL PROPERTIES OF FLUOROPHORES VIA PROXIMAL QUENCHING 审中-公开
标题翻译：通过邻位猝灭来改变荧光物质的光学物理性质的方法和组合物
公开(公告)号：WO2010096720A3
公开(公告)日：2010-11-18
申请号：PCT/US2010024824
申请日：2010-02-19
申请人： UNIV CORNELL , BLANCHARD SCOTT , ALTMAN ROGER , WARREN J DAVID
发明人： BLANCHARD SCOTT , ALTMAN ROGER , WARREN J DAVID
IPC分类号： C09K11/00
CPC分类号： A61K49/0052 , A61K49/0032 , A61K49/0041 , A61K49/0067 , G01N33/542
摘要： The invention is directed to fluorophore-containing compositions and configurations wherein proximity between the fluorophore and one or more protective agents (PAs) modifies the lifetime of fluorescent and/or dark states, their frequency of occurrence, and the total lifetime of fluorescence in order to appropriately modify the photophysical characteristics of the fluorophore. The invention is also directed to methods that utilize these compositions and configurations.
摘要翻译：本发明涉及含荧光团的组合物和构型，其中荧光团和一种或多种保护剂（PA）之间的接近度改变了荧光和/或黑态的寿命，其发生频率和荧光的总寿命，以便适当修改荧光团的光物理特性。本发明还涉及利用这些组合物和构造的方法。

2. 发明申请

WO2012103423A3 COMPUTER-IMPLEMENTED PLATFORM FOR AUTOMATED FLUORESCENCE IMAGING AND KINETIC ANALYSIS 审中-公开
标题翻译：用于自动荧光成像和动力学分析的计算机实现平台
公开(公告)号：WO2012103423A3
公开(公告)日：2013-01-17
申请号：PCT/US2012022875
申请日：2012-01-27
申请人： UNIV CORNELL , BLANCHARD SCOTT , TERRY DANIEL , MUNRO JAMES , GEGGIER PETER
发明人： BLANCHARD SCOTT , TERRY DANIEL , MUNRO JAMES , GEGGIER PETER
IPC分类号： G01N21/64 , C12M1/00 , G01N33/483
CPC分类号： G06T7/0012 , G01N21/6408 , G01N21/6456 , G01N2021/6441
摘要： Automatically selecting time traces from a fluorescence experiment, in one aspect, may include capturing results of the fluorescence experiment in a moving image; localizing sources of fluorescence in the moving image; producing time traces of each fluorescent source by monitoring fluorescence intensity of said localized sources in the moving image over time; removing unuseful time traces from said produced time traces; and selecting useful time traces from said produced time traces based on one or more defined criteria. FRET traces from selected time traces may be further calculated and analyzed. A unified computer-implemented platform in one aspect may include tools to locate single molecules, extract traces, classify smFRET traces according to adjustable parameters, and quantify the kinetic parameters of FRET transitions using analytical procedures such as Hidden Markov Modeling (HMM) procedures.
摘要翻译：在一个方面，从荧光实验自动选择时间迹线可以包括在运动图像中捕获荧光实验的结果; 在运动图像中定位荧光源; 通过随时间监测运动图像中的所述局部源的荧光强度来产生每个荧光源的时间轨迹; 从所述生成的时间痕迹中去除无用的时间痕迹; 以及基于一个或多个确定的标准从所述产生的时间迹线中选择有用的时间迹线。可以进一步计算和分析来自选定时间轨迹的FRET轨迹。在一个方面，统一的计算机实现的平台可以包括用于定位单分子的工具，提取痕迹，根据可调参数分类smFRET迹线，以及使用诸如隐马尔可夫模型（HMM）程序之类的分析程序量化FRET转变的动力学参数。

3. 发明申请

WO2011143575A3 smFRET WITH MEMBRANE PROTEINS 审中-公开
标题翻译： smFRET与膜蛋白
公开(公告)号：WO2011143575A3
公开(公告)日：2012-03-08
申请号：PCT/US2011036459
申请日：2011-05-13
申请人： UNIV CORNELL , UNIV COLUMBIA , BLANCHARD SCOTT , WEINSTEIN HAREL , JAVITCH JONATHAN
发明人： BLANCHARD SCOTT , WEINSTEIN HAREL , JAVITCH JONATHAN
IPC分类号： G01N33/68 , C07K14/00 , G01N33/52
CPC分类号： G01N33/542 , C07K14/001 , C07K14/195 , C07K14/245 , G01N21/6428 , G01N33/582 , G01N2500/04
摘要： This disclosure is directed to methods of conducting dynamic single-molecule fluorescence studies such as smFRET on a membrane protein which permits observation and quantification of conformational dynamics of a membrane protein. Also disclosed herein are mutant membrane proteins in which one or more mutations have been introduced for affixing a fluorophore, as well as reagents and kits containing such mutant membrane proteins for conducting dynamic single-molecule fluorescence studies. The methods and compositions disclosed herein can be used in screening for compounds that enhance or reduce the activity of a membrane protein, useful for treating diseases associated with the malfunction of the membrane protein or alterations in membrane protein conformation.
摘要翻译：本公开涉及在膜蛋白上进行动态单分子荧光研究如smFRET的方法，其允许观察和定量膜蛋白的构象动力学。本文还公开了突变体膜蛋白，其中已经引入一个或多个突变以固定荧光团，以及含有这种用于进行动态单分子荧光研究的突变膜蛋白的试剂和试剂盒。本文公开的方法和组合物可用于筛选增强或降低膜蛋白活性的化合物，所述蛋白质可用于治疗与膜蛋白的故障相关的疾病或膜蛋白构象的改变。

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