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1. 发明申请

WO2012079030A2 BIOCONJUGATION USING BIFUNCTIONAL LINKERS 审中-公开
标题翻译：使用双功能链接的BIOCONJUGATION
公开(公告)号：WO2012079030A2
公开(公告)日：2012-06-14
申请号：PCT/US2011064253
申请日：2011-12-09
申请人： UNIV CALIFORNIA , LIAO JIAYU , ZHAO YONGFENG
发明人： LIAO JIAYU , ZHAO YONGFENG
IPC分类号： G01N33/543 , C12Q1/68 , G01N33/52 , G01N33/532 , G01N33/544 , G01N33/553 , G01N33/68
CPC分类号： G01N33/552 , C07F7/1868 , C07K17/06 , G01N33/54353
摘要： A bifunctional linker and method of use is provided that has a spacer molecule with a functional group on one end configured to couple to the surface of a substrate and a function group on the other end that is configured couple to a biomolecule and methods of use. The preferred bifunctional linker has a poly(ethylene glycol) spacer ranging from 3 to 20 ethylene glycol units that has a silane functional group to react with a substrate and an azide functional group that can couple to a biomolecule that includes an alkyne group. The preferred linker can produce an azide-derivatized glass surface in one step and the azide functional group of the spacer can in sequence conjugate with a biomolecule using click chemistry, which can be conducted at low temperature and in aqueous solution.
摘要翻译：提供了双功能接头和使用方法，其具有间隔分子，所述间隔分子在一端上具有配置成耦合至基底表面的官能团，另一端上配置有偶联于生物分子的官能团以及使用方法。优选的双功能接头具有范围为3至20个乙二醇单元的聚乙二醇间隔基，其具有与基底反应的硅烷官能团和可以偶联至包含炔基的生物分子的叠氮化物官能团。优选的连接体可以在一个步骤中产生叠氮化物衍生化的玻璃表面，并且间隔体的叠氮化物官能团可以使用点击化学与生物分子序列缀合，这可以在低温下和在水溶液中进行。

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