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1. 发明申请

WO2012115454A3 METHOD FOR CONCENTRATING CELLS THAT ARE GENETICALLY ALTERED BY NUCLEASES 审中-公开
标题翻译：用于浓缩由细胞进行遗传改变的细胞的方法
公开(公告)号：WO2012115454A3
公开(公告)日：2012-12-20
申请号：PCT/KR2012001367
申请日：2012-02-22
申请人： TOOLGEN INC , SNU R&DB FOUNDATION , KIM JIN SOO , KIM SEOK JOONG , KIM YONG SUB
发明人： KIM JIN SOO , KIM SEOK JOONG , KIM YONG SUB
IPC分类号： C12Q1/34 , C12N9/14 , C12N15/63 , C12Q1/66
CPC分类号： C12Q1/44 , C12N15/64 , G01N33/50
摘要： The present invention relates to a reporter composition and to a method for composing the reporter for selecting or concentrating cells in which a specific nucleotide sequence in cells is cut by a specific nuclease, or the nucleotide sequence is altered by the cutting. The present invention also relates to host cells comprising the reporter composition, and to a system for monitoring nuclease activity. The reporter system of the present invention is simple and non-invasive, and enables the efficient concentration of genetically altered cells. Accordingly, the method of the present invention may promote the use of nucleases not only in gene treatment and genetic engineering, but also in basic research.
摘要翻译：本发明涉及一种报道组合物和组成报告物的方法，用于选择或浓缩细胞中特定核苷酸序列被特异性核酸酶切割或细胞通过切割而改变的细胞。本发明还涉及包含报道组合物的宿主细胞，以及用于监测核酸酶活性的系统。本发明的报告体系简单无侵害，能够有效地浓缩遗传改变细胞。因此，本发明的方法可以促进不仅在基因治疗和遗传工程中使用核酸酶，而且可以促进基础研究。

2. 发明申请

WO2012134241A3 ZINC FINGER NUCLEASE TARGETING THE CMAH GENE, AND A USE THEREFOR 审中-公开
标题翻译： ZINC指纹核心靶向CMAH基因及其用途
公开(公告)号：WO2012134241A3
公开(公告)日：2013-01-10
申请号：PCT/KR2012002419
申请日：2012-03-30
申请人： TOOLGEN INC , KIM JIN SOO , KIM SEOK JOONG
发明人： KIM JIN SOO , KIM SEOK JOONG
IPC分类号： C12N15/12 , A01K67/027 , C12N5/10 , C12N9/14 , C12N15/55 , C12N15/85
CPC分类号： C12N9/16 , A01K67/0276 , A01K2227/108 , A01K2267/025
摘要： The present invention relates to: a method for making pig cells from which the swine CMAH (swine cytidine monophospho-N-acetylneuraminic acid hydroxylase) gene has been knocked out; a method for making a pig from which the swine CMAH gene has been knocked out; a method for making a pig-derived artificial organ or artificial tissue from which the swine CMAH gene has been knocked out; a zinc finger nuclease targeting the CMAH gene; a polynucleotide coding for the zinc finger nuclease; a vector comprising the polynucleotide; a method for making cells from which the CMAH gene has been knocked out by using the zinc finger nuclease; and an egg of a nonhuman animal from which the CMAH gene has been knocked out, the nonhuman animal and a kit used to make an artificial organ derived from the nonhuman animal. The present invention makes it possible to obtain a large volume of transgenic cells for creating pigs for xenogeneic organ transplantation from which the CMAH gene is eliminated, by using a zinc finger nuclease for CMAH genes.
摘要翻译：本发明涉及一种猪细胞的制造方法，其中猪CMAH（猪胞苷磷脂酰磷酸N-乙酰神经氨酸羟化酶）基因被敲除; 猪CMAH基因已被敲除的猪的制备方法; 制造猪CMAH基因已被敲除的猪源性人造器官或人造组织的方法; 靶向CMAH基因的锌指核酸酶; 编码锌指核酸酶的多核苷酸; 包含多核苷酸的载体; 通过使用锌指核酸酶来制备CMAH基因已经被敲除的细胞的方法; 和非人动物的卵，其中CMAH基因已被敲除，非人动物和用于制备来自非人动物的人造器官的试剂盒。通过使用锌指核酸酶用于CMAH基因，本发明使得可以获得大量用于产生用于异种器官移植的猪的转基因细胞，其中CMAH基因从其中消除。

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