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1. 发明申请

WO0190337A3 DETECTION OF RNA 审中-公开
标题翻译：检测RNA
公开(公告)号：WO0190337A3
公开(公告)日：2003-01-23
申请号：PCT/US0117086
申请日：2001-05-24
申请人： THIRD WAVE TECH INC , ALLAWI HATIM , BARTHOLOMAY CHRISTIAN TOR , CHEHAK LUANNE , CURTIS MICHELLE L , EIS PEGGY S , HALL JEFF G , IP HON S , KAISER MICHAEL , KWIATKOWSKI ROBERT W JR , LUKOWIAK ANDREW A , LYAMICHEV VICTOR , MA WUPO , OLSON-MUNOZ MARILYN C , OLSON SARAH M , SCHAEFER JAMES J , SKRZYPCZYNSKI ZBIGNIEW , TAKOVA TSETSKA Y , VEDVIK KEVIN L , LYAMICHEV NATALIE E , NERI BRUCE P
发明人： ALLAWI HATIM , BARTHOLOMAY CHRISTIAN TOR , CHEHAK LUANNE , CURTIS MICHELLE L , EIS PEGGY S , HALL JEFF G , IP HON S , KAISER MICHAEL , KWIATKOWSKI ROBERT W JR , LUKOWIAK ANDREW A , LYAMICHEV VICTOR , MA WUPO , OLSON-MUNOZ MARILYN C , OLSON SARAH M , SCHAEFER JAMES J , SKRZYPCZYNSKI ZBIGNIEW , TAKOVA TSETSKA Y , VEDVIK KEVIN L , LYAMICHEV NATALIE E , NERI BRUCE P
IPC分类号： G01N21/78 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/12 , C12N9/22 , C12N15/09 , C12Q1/68 , C12R1/01
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译：本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。在一些实施方案中，使用多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。

2. 发明申请

WO0198537A3 NUCLEIC ACID ACCESSIBLE HYBRIDIZATION SITES 审中-公开
标题翻译：核酸可接受的混合网站
公开(公告)号：WO0198537A3
公开(公告)日：2003-10-16
申请号：PCT/US0119401
申请日：2001-06-15
申请人： THIRD WAVE TECH INC , LYAMICHEV VICTOR , ALLAWI HATIM , DONG FANG , NERI BRUCE P , VENER I TATIANA
发明人： LYAMICHEV VICTOR , ALLAWI HATIM , DONG FANG , NERI BRUCE P , VENER I TATIANA
IPC分类号： C07H21/04 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6827 , C12Q1/6811 , C12Q1/686 , C12Q1/703 , C12Q2535/125 , C12Q2525/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids, and in particular, methods and compositions for detection and characterization of nucleic acid sequences and sequence changes. The present invention also provides methods and compositions for identifying oligonucleotides with desired hybridization properties to nucleic acid targets containing secondary structure.
摘要翻译：本发明涉及用于分析核酸的方法和组合物，特别涉及用于检测和表征核酸序列和序列变化的方法和组合物。本发明还提供用于鉴定具有包含二级结构的核酸靶具有期望的杂交性质的寡核苷酸的方法和组合物。

3. 发明申请

WO2013058868A3 MULTIPLEXED KRAS MUTATION DETECTION ASSAY 审中-公开
标题翻译：多重KRAS突变检测测定
公开(公告)号：WO2013058868A3
公开(公告)日：2014-05-15
申请号：PCT/US2012052377
申请日：2012-08-24
申请人： EXACT SCIENCES CORP , OLDHAM-HALTOM REBECCA , ALLAWI HATIM , ZOU HONGZHI , DOMANICO MICHAEL J , LIDGARD GRAHAM P
发明人： OLDHAM-HALTOM REBECCA , ALLAWI HATIM , ZOU HONGZHI , DOMANICO MICHAEL J , LIDGARD GRAHAM P
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q2525/161 , C12Q2561/109 , C12Q2565/1015
摘要： Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.
摘要翻译：本文提供的试剂混合物包含多重扩增试剂和皮瓣测定试剂，用于在单次反应中检测含有34A，34C，34T，35A，35C，35T或38A点突变中任何一种的KRAS基因的突变体拷贝。还提供了使用试剂混合物和试剂盒进行其的方法。

4. 发明申请

WO2012067830A2 METHYLATION ASSAY 审中-公开
标题翻译：甲基化测定
公开(公告)号：WO2012067830A2
公开(公告)日：2012-05-24
申请号：PCT/US2011058997
申请日：2011-11-02
申请人： EXACT SCIENCES CORP , ZOU HONGZHI , LIDGARD GRAHAM P , DOMANICO MICHAEL J , ALLAWI HATIM
发明人： ZOU HONGZHI , LIDGARD GRAHAM P , DOMANICO MICHAEL J , ALLAWI HATIM
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q2600/154 , C12Q2521/301 , C12Q2523/125 , C12Q2561/109
摘要： A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3' terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.
摘要翻译：提供了一种检测甲基化基因组座位的方法。在某些实施方案中，所述方法包括：a）将含有基因组座位的非甲基化和甲基化拷贝的核酸样品用修饰胞嘧啶至尿嘧啶的试剂处理以产生经处理的核酸; b）使用第一引物和第二引物扩增来自经处理的核酸的产物，其中第一引物与含有甲基胞嘧啶的位点的位点杂交，并且扩增优先扩增基因组基因座的甲基化拷贝，以产生扩增的样品; 和c）使用采用具有对应于基因组基因座中的甲基化位点的3'末端G或C核苷酸的侵入性寡核苷酸的皮瓣测定来检测扩增样品中扩增的基因组座位的扩增的甲基化拷贝的存在。

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