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1. 发明申请

WO1993005164A1 CALLUS-SPECIFIC PROMOTERS 审中-公开
标题翻译：电话特价促销
公开(公告)号：WO1993005164A1
公开(公告)日：1993-03-18
申请号：PCT/GB1992001602
申请日：1992-09-02
申请人： THE UNIVERSITY OF LEICESTER , DRAPER, John
发明人： THE UNIVERSITY OF LEICESTER
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： A marker gene for use in the genetic transformation of plants is driven by a promoter which is capable of acting as a callus-specific promoter, for example in monocotyledonous plants. A suitable promoter naturally drives the expression of a gene encoding a predicted 16.92 kDa protein after wounding and/or callus formation in Asparagus officinalis or an equivalent protein in other members of Liliaceae or Amaryllidaceae. A preferred embodiment is designated the AoPR1 promoter. Under the control of a promoter of the invention, the marker gene (which may for example be an antibiotic- or herbicide-resistance gene) is expressed strongly in wounded tissues and cells and cultured explants, but not constitutively throughout the whole plant. Such promoters may also be used to drive expression of genes in plant tissue culture.
摘要翻译：用于植物遗传转化的标记基因由能够作为愈伤组织特异性启动子的启动子驱动，例如在单子叶植物中。合适的启动子自然地驱动编码在芦笋中的伤口和/或愈伤组织形成之后的预测的16.92kDa蛋白质的基因的表达或百合科或芳樟科的其他成员中的等同蛋白质的表达。优选的实施方案指定为AoPR1启动子。在本发明的启动子的控制下，标记基因（其可以是例如抗生素或除草剂抗性基因）在受伤的组织和细胞和培养的外植体中强烈表达，但不整体在整个植物中表达。此类启动子也可用于驱动植物组织培养中基因的表达。

2. 发明申请

WO1998001560A1 MODIFIED PROTEIN G AND FRAGMENTS THEREOF 审中-公开
标题翻译：改性蛋白G及其片段
公开(公告)号：WO1998001560A1
公开(公告)日：1998-01-15
申请号：PCT/GB1997001818
申请日：1997-07-04
申请人： THE UNIVERSITY OF MANCHESTER INSTITUTE OF SCIENCE & TECHNOLOGY , THE UNIVERSITY OF LEICESTER , DERRICK, Jeremy , ROBERTS, Gordon
发明人： THE UNIVERSITY OF MANCHESTER INSTITUTE OF SCIENCE & TECHNOLOGY , THE UNIVERSITY OF LEICESTER
IPC分类号： C12N15/31
CPC分类号： C07K1/22 , C07K14/315 , C07K16/065
摘要： A polypeptide sequence which is a modified form of protein G or fragment thereof which selectively binds to one of the Fab or Fc fragments of an antibody relative to the other as compared to the corresponding selectivity of binding of unmodified protein G.
摘要翻译：与未修饰蛋白G的结合的相应选择性相比，多肽序列是蛋白G或其片段的修饰形式，其选择性地结合抗体相对于另一抗体的Fab或Fc片段之一。

3. 发明申请

WO1990008827A1 REGULATION OF GENE EXPRESSION 审中-公开
标题翻译：基因表达调控
公开(公告)号：WO1990008827A1
公开(公告)日：1990-08-09
申请号：PCT/GB1990000115
申请日：1990-01-26
申请人： IMPERIAL CHEMICAL INDUSTRIES PLC , THE UNIVERSITY OF LEICESTER
发明人： IMPERIAL CHEMICAL INDUSTRIES PLC , THE UNIVERSITY OF LEICESTER , BRIDGES, Ian, George , BRIGHT, Simon, William, Jonathan , GREENLAND, Andrew, James , SCHUCH, Wolfgang, Walter , PIOLI, David , MERRYWEATHER, Andrew
IPC分类号： C12N15/67
CPC分类号： C12N15/8216 , C12N15/635 , C12N15/67 , C12N15/72 , C12N15/73 , C12N15/8217 , C12N15/8238
摘要： Pseudo-operator sequences may be located in (or inserted into) plant genomes and utilised to drive expression of foreign genes. These pseudo-operator sequences are nucleotide sequences which are present at a suitable location in a gene at which repressor binding will lead to inhibitation or enhancement of gene expression. The disclosed technique permits the design of altered specificity repressors, which bind the pseudo-operators.
摘要翻译：伪操作者序列可以位于（或插入）植物基因组中，并用于驱动外源基因的表达。这些伪操作序列是存在于抑制子结合将导致基因表达的抑制或增强的基因中的合适位置处的核苷酸序列。所公开的技术允许设计改变的特异性阻遏物，其结合伪操作者。

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