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1. 发明申请

WO2004065617A2 HAPLOTYPE ANALYSIS 审中-公开
标题翻译： HAPLOTYPE分析
公开(公告)号：WO2004065617A2
公开(公告)日：2004-08-05
申请号：PCT/US2004/001329
申请日：2004-01-16
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , DING, Chunming
发明人： CANTOR, Charles, R. , DING, Chunming
IPC分类号： C12Q
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C12Q2527/137 , C12Q2537/143 , C12Q2565/627
摘要： The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.
摘要翻译：本发明提供了高通量单倍型分析的有效方法。可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定的单倍体存在于受试者。核酸标记在染色体上可以彼此有任何距离。此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

2. 发明申请

WO2006019407A2 METHOD FOR DETECTING AND QUANTIFYING RARE MUTATIONS/POLYMORPHISMS 审中-公开
标题翻译：检测和量化稀有突变/多态性的方法
公开(公告)号：WO2006019407A2
公开(公告)日：2006-02-23
申请号：PCT/US2005/005255
申请日：2005-02-18
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , DING, Chunming
发明人： CANTOR, Charles, R. , DING, Chunming
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2600/156 , C12Q2525/186 , C12Q2535/125 , C12Q2565/627 , C12Q2545/101
摘要： The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).
摘要翻译：本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。正在研究的核酸分子可以是DNA或RNA。罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变（S）。

3. 发明申请

WO2005023091A2 METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS 审中-公开
标题翻译：非侵入性预后诊断方法
公开(公告)号：WO2005023091A2
公开(公告)日：2005-03-17
申请号：PCT/US2004/028857
申请日：2004-09-07
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , THE CHINESE UNIVERSITY OF HONG KONG , CANTOR, Charles, R. , DING, Chunming , LO, Yuk, Ming, Dennis , CHIU, Rossa, Wai-Kwun
发明人： CANTOR, Charles, R. , DING, Chunming , LO, Yuk, Ming, Dennis , CHIU, Rossa, Wai-Kwun
IPC分类号： A61B
CPC分类号： C12Q1/6881 , C12Q1/6872 , C12Q2600/156 , C12Q2600/172 , C12Q2535/125
摘要： The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in biological sample.
摘要翻译：本发明涉及检测生物样品中核酸的方法。该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。该方法可用于例如诊断，预后和治疗目的。该方法可用于例如诊断，预后和治疗目的。该方法允许精确检测在生物样品中以非常少的量存在的核酸。例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和供体特异性酸在移植受体。在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其它外来核酸。

4. 发明申请

WO2004022721A2 QUANTIFICATION OF GENE EXPRESSION 审中-公开
标题翻译：基因表达的定量
公开(公告)号：WO2004022721A2
公开(公告)日：2004-03-18
申请号：PCT/US2003/028081
申请日：2003-09-05
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , DING, Chunming
发明人： CANTOR, Charles, R. , DING, Chunming
IPC分类号： C12N
CPC分类号： C12Q1/6851 , C12Q2545/107 , C12Q2535/125 , C12Q2525/186
摘要： The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a "target nucleic acid sequence." Use of such standard in combination with a method of "enhancing" the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the "enhanced" standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.
摘要翻译：本发明涉及使用设计为与目的基因相比具有一个碱基差的标准或“靶核酸序列”来测量样品中靶核酸量的方法。使用这样的标准物与“增强”标准差异的方法和使用例如在突变位点上携带的碱基延伸反应的测试核酸样品的方法相结合，可以扩增标准品和靶核酸相同的效率和促进目标核酸的定量。此后，使用定量“增强”标准和靶核酸样品的手段来确定靶核酸的量。在优选实施方案中，定量方法是质谱法。

5. 发明申请

WO2005035725A2 METHODS FOR PRENATAL DIAGNOSIS OF CHROMOSOMAL ABNORMALITIES 审中-公开
标题翻译：用于临床诊断染色体异常的方法
公开(公告)号：WO2005035725A2
公开(公告)日：2005-04-21
申请号：PCT/US2004/033175
申请日：2004-10-08
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , DING, Chunming
发明人： CANTOR, Charles, R. , DING, Chunming
IPC分类号： C12N
CPC分类号： C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/6876 , C12Q2600/154 , C12Q2600/156 , C12Q2600/166 , C12Q2521/331
摘要： Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.
摘要翻译：染色体异常负责大量的出生缺陷，包括精神发育迟滞。本发明涉及基于母体血液样品分析的非侵入性和快速，产前诊断染色体异常的方法。本发明利用母体和胎儿之间的DNA差异，例如甲基化状态的差异，作为富集母体血浆样品中胎儿DNA的手段。本文描述的方法可用于检测染色体DNA缺失和重复。在优选的实施方案中，所述方法用于诊断染色体非整倍体和相关疾病，例如唐氏和特纳综合征。

6. 发明申请

WO2007132167A2 NOVEL MARKERS FOR PRENATAL DIAGNOSIS AND MONITORING 审中-公开
标题翻译：用于临床诊断和监测的新标志
公开(公告)号：WO2007132167A2
公开(公告)日：2007-11-22
申请号：PCT/GB2007/001627
申请日：2007-05-03
申请人： THE CHINESE UNIVERSITY OF HONG KONG , LO, Yuk Ming Dennis , CHIU, Rossa Wai Kwun , CHIM, Stephen Siu Chung , DING, Chunming , JIN, Shengnan , LEE, Tracy Yuen Han , LUN, Fiona Miu Fun
发明人： LO, Yuk Ming Dennis , CHIU, Rossa Wai Kwun , CHIM, Stephen Siu Chung , DING, Chunming , JIN, Shengnan , LEE, Tracy Yuen Han , LUN, Fiona Miu Fun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2523/125
摘要： This application provides the use of novel fetal markers for prenatal diagnosis and monitoring of certain pregnancy-related conditions. More specifically, the invention resides in the discovery that certain CpG islands located on fetal chromosome 21 demonstrate a methylation profile that is distinct from that of the corresponding CpG islands located on maternal chromosome 21. This application also provides kits for diagnosing or monitoring of the relevant conditions.
摘要翻译：该应用提供了新颖的胎儿标记物用于产前诊断和监测某些妊娠相关病症的用途。更具体地，本发明在于发现位于胎儿染色体21上的某些CpG岛表现出与位于母体染色体21上的相应CpG岛不同的甲基化谱。该应用还提供用于诊断或监测相关条件。

7. 发明申请

WO2003068828A1 SOLID CATALYST COMPONENT FOR POLYMERIZATION OF OLEFINS, CATALYST COMPRISING THE SAME AND USE THEREOF 审中-公开
标题翻译：用于烯烃聚合的固体催化剂组分，包含其的催化剂及其用途
公开(公告)号：WO2003068828A1
公开(公告)日：2003-08-21
申请号：PCT/CN2003/000110
申请日：2003-01-30
申请人： CHINA PETROLEUM & CHEMICAL CORPORATION , BEIJING RESEARCH INSTITUTE OF CHEMICAL INDUSTRY, CHINA PETROLEUM & CHEMICAL CORPORATION , GAO, Mingzhi , LIU, Haitao , LI, Zhulan , WANG, Jun , YANG, Juxiu , LI, Tianyi , WANG, Xiaodong , LI, Changxiu , DING, Chunming
发明人： GAO, Mingzhi , LIU, Haitao , LI, Zhulan , WANG, Jun , YANG, Juxiu , LI, Tianyi , WANG, Xiaodong , LI, Changxiu , DING, Chunming
IPC分类号： C08F4/654
CPC分类号： C08F10/00 , C08F110/06 , C08F4/651 , C08F4/6494 , C08F4/6492 , C08F4/6465 , C08F2500/18 , C08F2500/12 , C08F2500/15 , C08F2500/04
摘要： The present invention provides a solid catalyst component for the polymerization of olefins, comprising magnesium, titanium, a halogen and an electron donor, wherein said electron donor comprises at least one selected from the group consisting of polyol ester compounds of the formula (I): R 1 CO-O-CR 3 R 4 -A-CR 5 R 6 -O-CO-R 2 (I) wherein, R 1 and R 2 groups, which may be identical or different, can be substituted or unsubstituted hydrocarbyl having 1 to 20 carbon atoms, R 3 -R 6 groups, which may be identical or different, can be selected from the group consisting of hydrogen, halogen or substituted or unsubstituted hydrocarbyl having 1 to 20 carbon atoms, R 1 -R 6 groups optionally contain one or more hetero-atoms replacing carbon, hydrogen atom or the both, said hetero-atom is selected from the group consisting of nitrogen, oxygen, sulfur, silicon, phosphorus and halogen atom, two or more of R 3 -R 6 groups can be linked to form saturated or unsaturated monocyclic or polycyclic ring; A is a single bond or bivalent linking group with chain length between two free radicals being 1-10 atoms, wherein said bivalent linking group is selected from the group consisting of aliphatic, alicyclic and aromatic bivalent radicals, and can carry C 1 -C 20 linear or branched substituents; one or more of carbon atom and/or hydrogen atom on above-mentioned bivalent linking group and substituents can be replaced by a hetero-atom selected from the group consisting of nitrogen, oxygen, sulfur, silicon, phosphorus, and halogen atom, and two or more said substituents on the linking group as well as above-mentioned R 3 -R 6 groups can be linked to form saturated or unsaturated monocyclic or polycyclic ring.
摘要翻译：本发明提供了一种用于烯烃聚合的固体催化剂组分，其中包括镁，钛，卤素和电子给体，其中所述电子给体包括选自由式I表示的多元醇酯化合物：R1CO- O-CR 3 R 4 -A-CR 5 R 6 -O-CO-R 2 I，其中可以相同或不同的R 1和R 2可以是具有1至20个碳原子的取代或未取代的烃基，可以相同的R 3 -R 6基团或不同的，可以选自氢，卤素或具有1至20个碳原子的取代或未取代的烃基，R 1 -R 6基团任选地含有一个或多个取代碳，氢原子或二者的杂原子，原子选自氮，氧，硫，硅，磷和卤素原子，两个或多个R 3 -R 6基团可以连接形成饱和或不饱和单环或多环A是单键或双键其中两个自由基之间的链长为1-10个原子，其中所述二价连接基团选自脂族，脂环族和芳族二价基团，并且可以携带C1-C20直链或支链取代基的一个或多个上述二价连接基团和取代基上的碳原子和/或氢原子可以被选自氮，氧，硫，硅，磷和卤素原子的杂原子取代，并且两个或更多个所述取代基在连接基团上以及上述R3-R6基团可连接形成饱和或不饱和单环或多环。

8. 发明申请

WO2009030100A1 METHODS AND KITS FOR SELECTIVELY AMPLIFYING, DETECTING OR QUANTIFYING TARGET DNA WITH SPECIFIC END SEQUENCES 审中-公开
标题翻译：用于选择性扩增，检测或定量具有特定末端序列的靶标DNA的方法和工具
公开(公告)号：WO2009030100A1
公开(公告)日：2009-03-12
申请号：PCT/CN2008/001423
申请日：2008-08-05
申请人： THE CHINESE UNIVERSITY OF HONG KONG , LO, Yuk Ming Dennis , TONG, Yu Kwan , CHIU, Wai Kwun Rossa , DING, Chunming
发明人： LO, Yuk Ming Dennis , TONG, Yu Kwan , CHIU, Wai Kwun Rossa , DING, Chunming
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2525/301 , C12Q2521/331
摘要： Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.
摘要翻译：本文公开了用于选择性扩增，检测或定量具有特异末端序列的DNA片段的方法和试剂盒，特别是在限制酶消化后产生。该方法可用于例如检测低甲基化DNA片段。该方法和试剂盒特别可用于检测或定量含有相应的高甲基化母体DNA片段的母体血浆样品中的低甲基化胎儿DNA片段。

9. 发明申请

WO2005118852A2 A MARKER FOR PRENATAL DIAGNOSIS AND MONITORING 审中-公开
标题翻译：用于临床诊断和监测的标记
公开(公告)号：WO2005118852A2
公开(公告)日：2005-12-15
申请号：PCT/GB2005/002211
申请日：2005-06-06
申请人： THE CHINESE UNIVERSITY OF HONG KONG , LO, Yuk Ming Dennis , CHIU, Rossa Wai Kwun , CHIM, Stephen Siu Chung , TONG, Yu-kwan , DING, Chunming
发明人： LO, Yuk Ming Dennis , CHIU, Rossa Wai Kwun , CHIM, Stephen Siu Chung , TONG, Yu-kwan , DING, Chunming
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2521/331
摘要： The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.
摘要翻译：本发明涉及通过分析存在于母亲血液中的胎儿DNA来诊断妊娠相关疾病的新方法。更具体地说，本发明依赖于发现maspin基因在胎儿DNA和母体DNA中差异甲基化，并提供了这些新的诊断方法，其将胎儿DNA与母体DNA区分开来，并根据胎儿DNA水平和甲基化异常检测产前疾病状态。

10. 发明申请

WO2008038000A1 DIAGNOSTIC METHOD FOR DETECTING OR MONITORING CANCER 审中-公开
标题翻译：用于检测或监测癌症的诊断方法
公开(公告)号：WO2008038000A1
公开(公告)日：2008-04-03
申请号：PCT/GB2007/003674
申请日：2007-09-26
申请人： THE CHINESE UNIVERSITY OF HONG KONG , HO, Yuk, Ming, Dennis , CHAN, Kwan, Chee, Allen , DING, Chunming
发明人： HO, Yuk, Ming, Dennis , CHAN, Kwan, Chee, Allen , DING, Chunming
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/118 , C12Q2600/154
摘要： The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.
摘要翻译：本发明涉及使用选自血液，血浆，血清，唾液，个体尿液的生物样品检测或监测癌症的方法，所述方法包括：（a）从所述生物样品中获得DNA; （b）用一种或多种甲基化敏感性限制酶消化DNA样品; （c）在步骤（b）之后量化或检测目标DNA序列，其中所述靶序列含有至少两个甲基化敏感性限制酶识别位点; 和（d）将来自个体的DNA序列的水平与正常标准进行比较，以检测，预测或监测癌症。

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