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    • 4. 发明申请
    • MATRIX MILL FOR DNA EXTRACTION
    • 用于DNA提取的MATRIX MILL
    • WO1998020164A1
    • 1998-05-14
    • PCT/US1997020156
    • 1997-11-04
    • CORNELL RESEARCH FOUNDATION, INC.
    • CORNELL RESEARCH FOUNDATION, INC.WEEDEN, Norman, F.LOOMIS, DaleCELESTE, Joseph, A.
    • C12Q01/68
    • B01L3/5085B03C1/24C12M47/06G01N1/286G01N35/0098
    • The device and methods herein disclosed were developed to allow a large number of small samples of organic tissue to be processed. The goal was to allow for the capture of these intracellular contents for study, use, and/or amplification. Though the device and specific protocols can be used to retrieve a variety of intracellular molecules one of the preferred embodiments particularly lends itself to the extraction of DNA. The electromagnetic device uses a set of terraced coils controlled in such a manner as to continually alternate polarity and thereby emulate the random motion of a manual mortar and pestle. This simulation of random motion is created through the arrangement of coils found in this invention. Methods were also developed to aid in the retrieval of DNA or other intracellular components using this device such that these components can be isolated and then used for study, amplification, or a variety of different purposes. Essentially the enclosed invention discloses a device and methodology which will allow for the rapid processing of many small tissue samples. This will assist in making such procedures as genotyping, genetic analysis, and gene mapping faster and more reliable on a large scale.
    • 本文公开的装置和方法被开发以允许处理大量有机组织的小样本。 目的是允许这些细胞内内容物的捕获用于研究,使用和/或扩增。 尽管该装置和具体方案可用于检索各种细胞内分子,但优选实施方案中的一个特别适用于提取DNA。 电磁装置使用一组梯形线圈,其以这样的方式进行控制,以连续地交替极性,从而模拟手动砂浆和杵的随机运动。 随机运动的这种模拟通过本发明中发现的线圈的布置产生。 还开发了方法以帮助使用该装置检索DNA或其他细胞内组分,使得这些组分可以被分离,然后用于研究,扩增或各种不同的目的。 本质上,所附的发明公开了一种将允许许多小组织样品的快速处理的装置和方法。 这将有助于大规模地进行基因分型,遗传分析和基因定位等过程更快更可靠的程序。
    • 5. 发明申请
    • KSHV POSITIVE CELL LINES
    • KSHV正电池线
    • WO1998012341A1
    • 1998-03-26
    • PCT/US1997016282
    • 1997-09-15
    • CORNELL RESEARCH FOUNDATION, INC.
    • CORNELL RESEARCH FOUNDATION, INC.CESARMAN, EthelARVANITAKIS, LeandrosKNOWLES, Daniel, M.MESRI, Enrique
    • C12P07/62
    • C12N7/00C12N2710/16451
    • The invention provides a cell line comprising Kaposi's sarcoma-associated herpesvirus (KSHV). Preferably, the cell line is a body cavity-based lymphoma cell line, and more preferably, the cell line does not harbor Epstein-Barr virus. Two cell lines designated BC-2 and BC-3 are thus provided. These cell lines can be used in a method of propagating KSHV. The method comprises culturing the cell line, wherein the KSHV within the cell line thereby propagates. The BC-3 cell line, in particular, can be used in a method of propagating KSHV in the absence of Epstein-Barr virus. The method comprises culturing the cell line designated BC-3, wherein the KSHV within the cell line thereby propagates. Further provided is a purified viral suspension of KSHV, as well as a composition comprising purified KSHV and a suitable carrier.
    • 本发明提供了包含卡波西肉瘤相关疱疹病毒(KSHV)的细胞系。 优选地,细胞系是基于体腔的淋巴瘤细胞系,更优选地,细胞系不包含爱泼斯坦 - 巴尔病毒。 因此提供了两个指定为BC-2和BC-3的细胞系。 这些细胞系可以用于传播KSHV的方法。 该方法包括培养细胞系,其中细胞系内的KSHV由此传播。 特别是BC-3细胞系可以用于在没有爱泼斯坦 - 巴尔病毒的情况下传播KSHV的方法。 该方法包括培养称为BC-3的细胞系,其中细胞系内的KSHV由此传播。 还提供了KSHV的纯化病毒悬浮液,以及包含纯化的KSHV和合适的载体的组合物。
    • 7. 发明申请
    • ELECTROSPRAY IONIZATION SOURCE AND METHOD OF USING THE SAME
    • 电沉积离子源及其使用方法
    • WO1998008613A1
    • 1998-03-05
    • PCT/US1997014997
    • 1997-08-27
    • CORNELL RESEARCH FOUNDATION, INC.VALASKOVIC, Gary, A.McLAFFERTY, Fred, W.
    • CORNELL RESEARCH FOUNDATION, INC.
    • B05B05/00
    • H01J49/167B05B5/0255G01N27/44717
    • An ultra-low flow rate electrospray ionization (ESI) source provides flow rates in the range of 1.0 nL/min or less. The source is comprised of a needle (12) which is fabricated by laser-heated pulling of fused-silica tubing, followed by chemical etching and surface metallization. The pulling results in formation of a slowly tapering capillary (40) within the needle (12) which tapers to a tip (22) having a very small inner diameter. The etching process sharpens the outer wall (42) of the needle (12) to a very sharp tip, and the combination of these parameters results in the ultra-low flow rate capability. After a metal electrical contact (44) is formed on the exterior wall of the needle (12), an electrically insulating overcoating (46) is preferably deposited thereon which locks the contact (44) in place, thereby greatly increasing needle life, and also restricting the electrical contact point to the very tip of the needle (12). Although the use of the ultra-low flow rate ESI sources increases sensitivity to sampling errors, a mechanism is also provided to minimize one primary source of such errors, evaporation induced hydrodynamic flow, in capillary electrophoresis (CE). An injection system (60) is provided which enables a retractable droplet of buffer solvent to be positioned in contact with the tip end of the ESI needle during sample loading. This prevents evaporation from the tip end, thereby eliminating hydrodynamic flow into the distal end (63) of the capillary column (62) used in the CE process.
    • 超低流速电喷雾电离(ESI)源提供1.0nL / min以下的流速。 该源由针(12)组成,其通过激光加热熔化石英管制造,随后进行化学蚀刻和表面金属化。 牵引导致在针(12)内形成缓慢变细的毛细管(40),其逐渐变细到具有非常小的内径的尖端(22)。 蚀刻工艺将针(12)的外壁(42)锐化到非常尖的尖端,并且这些参数的组合导致超低流速能力。 在针(12)的外壁上形成金属电接触(44)之后,优选在其上沉积电绝缘的外涂层(46),其将触点(44)锁定就位,从而大大增加针的寿命,并且还 将电接触点限制到针(12)的尖端。 尽管使用超低流量ESI源增加了对采样误差的敏感性,但也提供了一种机制来最小化毛细管电泳(CE)中这种错误,蒸发诱导的流体动力学流动的一个主要来源。 提供了一种注射系统(60),其在样品加载期间使缓冲溶剂的可收缩液滴定位成与ESI针的末端接触。 这防止了从尖端的蒸发,从而消除了进入CE过程中使用的毛细管柱(62)的远端(63)的流体动力学流动。