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1. 发明申请

WO1998020164A1 MATRIX MILL FOR DNA EXTRACTION 审中-公开
标题翻译：用于DNA提取的MATRIX MILL
公开(公告)号：WO1998020164A1
公开(公告)日：1998-05-14
申请号：PCT/US1997020156
申请日：1997-11-04
申请人： CORNELL RESEARCH FOUNDATION, INC.
发明人： CORNELL RESEARCH FOUNDATION, INC. , WEEDEN, Norman, F. , LOOMIS, Dale , CELESTE, Joseph, A.
IPC分类号： C12Q01/68
CPC分类号： B01L3/5085 , B03C1/24 , C12M47/06 , G01N1/286 , G01N35/0098
摘要： The device and methods herein disclosed were developed to allow a large number of small samples of organic tissue to be processed. The goal was to allow for the capture of these intracellular contents for study, use, and/or amplification. Though the device and specific protocols can be used to retrieve a variety of intracellular molecules one of the preferred embodiments particularly lends itself to the extraction of DNA. The electromagnetic device uses a set of terraced coils controlled in such a manner as to continually alternate polarity and thereby emulate the random motion of a manual mortar and pestle. This simulation of random motion is created through the arrangement of coils found in this invention. Methods were also developed to aid in the retrieval of DNA or other intracellular components using this device such that these components can be isolated and then used for study, amplification, or a variety of different purposes. Essentially the enclosed invention discloses a device and methodology which will allow for the rapid processing of many small tissue samples. This will assist in making such procedures as genotyping, genetic analysis, and gene mapping faster and more reliable on a large scale.
摘要翻译：本文公开的装置和方法被开发以允许处理大量有机组织的小样本。目的是允许这些细胞内内容物的捕获用于研究，使用和/或扩增。尽管该装置和具体方案可用于检索各种细胞内分子，但优选实施方案中的一个特别适用于提取DNA。电磁装置使用一组梯形线圈，其以这样的方式进行控制，以连续地交替极性，从而模拟手动砂浆和杵的随机运动。随机运动的这种模拟通过本发明中发现的线圈的布置产生。还开发了方法以帮助使用该装置检索DNA或其他细胞内组分，使得这些组分可以被分离，然后用于研究，扩增或各种不同的目的。本质上，所附的发明公开了一种将允许许多小组织样品的快速处理的装置和方法。这将有助于大规模地进行基因分型，遗传分析和基因定位等过程更快更可靠的程序。

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