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1. 发明申请

WO2009114185A2 METHODS AND COMPOSITIONS FOR TRANSLATIONAL PROFILING AND MOLECULAR PHENOTYPING 审中-公开
标题翻译：用于翻译分析和分子鉴定的方法和组合
公开(公告)号：WO2009114185A2
公开(公告)日：2009-09-17
申请号：PCT/US2009/001614
申请日：2009-03-12
申请人： THE ROCKEFELLER UNIVERSITY , HEINTZ, Nathaniel , GREENGARD, Paul , HEIMAN, Myriam , SCHAEFER, Anne , DOYLE, Joseph, P.n , DOUGHERTY, Joseph, D.
发明人： HEINTZ, Nathaniel , GREENGARD, Paul , HEIMAN, Myriam , SCHAEFER, Anne , DOYLE, Joseph, P.n , DOUGHERTY, Joseph, D.
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12N15/62 , A01K67/0278 , A01K2217/206 , A01K2227/105 , A01K2267/01 , C12N15/111 , C12N2320/12 , G01N33/5023 , G01N33/5058 , G01N2800/285
摘要： Methods and compositions are provided for translational profiling and molecular phenotyping of specific tissues, cells and cell subtypes of interest. The methods provided herein facilitate the analysis of gene expression in the selected subset present within a heterogeneous sample.
摘要翻译：提供了用于特定组织，细胞和感兴趣的细胞亚型的翻译分析和分子表型的方法和组合物。本文提供的方法有助于分析异种样品中存在的所选子集中的基因表达。

2. 发明申请

WO2003038049A3 METHOD FOR ISOLATING CELL-TYPE SPECIFIC MRNAS 审中-公开
公开(公告)号：WO2003038049A3
公开(公告)日：2003-05-08
申请号：PCT/US2002/034645
申请日：2002-10-29
申请人： RENOVIS, INC. , HEINTZ, Nathaniel , SERAFINI, Tito, A. , SHYJAN, Andrew, W.
发明人： HEINTZ, Nathaniel , SERAFINI, Tito, A. , SHYJAN, Andrew, W.
IPC分类号： C12Q1/68
摘要： The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA bound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis. The methods of the invention facilitate the analysis and quantification of gene expression in the selected cell type present within a heterogeneous cell mixture, without the need to isolate the cells of that cell type as a preliminary step.

3. 发明申请

WO2003038049A2 METHOD FOR ISOLATING CELL-TYPE SPECIFIC MRNAS 审中-公开
标题翻译：分离细胞型特异性MRNAS的方法
公开(公告)号：WO2003038049A2
公开(公告)日：2003-05-08
申请号：PCT/US2002/034645
申请日：2002-10-29
申请人： RENOVIS, INC. , HEINTZ, Nathaniel , SERAFINI, Tito, A. , SHYJAN, Andrew, W.
发明人： HEINTZ, Nathaniel , SERAFINI, Tito, A. , SHYJAN, Andrew, W.
IPC分类号： C12N
CPC分类号： C12N15/1041 , C12N15/1006 , C12N15/82 , C12N15/8222 , C12Q1/6827
摘要： The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA bound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis. The methods of the invention facilitate the analysis and quantification of gene expression in the selected cell type present within a heterogeneous cell mixture, without the need to isolate the cells of that cell type as a preliminary step.
摘要翻译：本发明提供了通过选择性分离以细胞类型特异性方式结合mRNA的核糖体或蛋白质分离细胞型特异性mRNA的方法，从而提供与结合mRNA的核糖体或蛋白质结合的mRNA。作为核糖体复合物的核糖体结合正在细胞中积极翻译的mRNA。根据本发明的方法，将细胞工程化以表达分子标记的核糖体蛋白质或蛋白质，其通过向细胞中引入核酸，所述核酸包含编码核糖体蛋白质或蛋白质的核苷酸序列，所述核糖体蛋白质或蛋白质与编码肽标签。然后可以分离标记的核糖体或mRNA结合蛋白以及与标记的核糖体或mRNA结合蛋白结合的mRNA，并分离mRNA并进一步用于基因表达分析。本发明的方法促进了在异质细胞混合物中存在的所选细胞类型中的基因表达的分析和定量，而不需要将该细胞类型的细胞作为初步步骤分离。

4. 发明申请

WO2018148501A1 METHODS FOR CELL-TYPE SPECIFIC PROFILING TO IDENTIFY DRUG TARGETS 审中-公开
公开(公告)号：WO2018148501A1
公开(公告)日：2018-08-16
申请号：PCT/US2018/017556
申请日：2018-02-09
申请人： THE ROCKEFELLER UNIVERSITY
发明人： HEINTZ, Nathaniel , XU, Xiao
IPC分类号： C12N15/11 , C12Q1/68
摘要： The present invention provides methods of profiling gene and protein expression of a plurality of nuclei from a single cell type and comparing the profiles to determine variability among cell populations, samples from different subjects, and cells expressing a disease phenotype.

5. 发明申请

WO1992003479A1 REPLICATION INITIATOR PROTEIN COMPLEX AND METHODS OF USE THEREOF 审中-公开
标题翻译：复制发射体蛋白复合物及其使用方法
公开(公告)号：WO1992003479A1
公开(公告)日：1992-03-05
申请号：PCT/US1991006119
申请日：1991-08-27
申请人： THE ROCKEFELLER UNIVERSITY , UNIVERSITY OF VERMONT
发明人： THE ROCKEFELLER UNIVERSITY , UNIVERSITY OF VERMONT , HEINTZ, Nathaniel , HEINTZ, Nicholas, H. , DAILEY, Lisa, A. , CADDLE, Mark, S.
IPC分类号： C07K15/06
CPC分类号： C12N9/90 , A61K38/00 , C07K14/47 , C07K14/4705 , C07K16/18 , C07K16/40 , G01N33/6875
摘要： A replication initiator protein complex for eukaryotic cells is disclosed which includes protein fractions of about 60 kD and 100 kD size. The protein complex comprises a protein in purified form that is capable of origin-specific DNA binding and ATP-dependent DNA helicase activity. The 60 kD fraction is named RIP60 and is primarily active in origin-specific DNA binding. The 100 kD fraction is named RIP100 and is primarily active as a DNA helicase. The protein complex and its fractions are active only during S phase and thereby commend their investigation and use toward the development of specific diagnostic and therapeutic modalities against pathogens to which cell division is critical. Diagnostic and therapeutic utilities are accordingly proposed, and testing procedures, materials in kit form, recombinant materials and procedures, and pharmaceutical compositions are likewise set forth.

6. 发明申请

WO1998022623A2 CDNA COLLECTIONS ENCODING PROTEINS REGULATED DURING PROGRAMMED CELL DEATH, AND METHODS OF USE THEREOF 审中-公开
标题翻译： CDNA收集编程细胞死亡调节蛋白质及其使用方法
公开(公告)号：WO1998022623A2
公开(公告)日：1998-05-28
申请号：PCT/US1997021132
申请日：1997-11-18
申请人： THE ROCKEFELLER UNIVERSITY , UNIVERSITY OF CALIFORNIA
发明人： THE ROCKEFELLER UNIVERSITY , UNIVERSITY OF CALIFORNIA , HEINTZ, Nathaniel , GUBBAY, John , SKINNER, Michael, K.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6876 , C12Q2600/158
摘要： The present invention identifies cDNA collections enriched in genes regulated in prostate homeostasis, prostate regression, and in genes regulated in programmed cell death. These novel cDNA collections provide cDNAs that encode proteins that are either unique to general programmed cell death or unique to prostate regression. These transcripts can be used as markers to screen, monitor and/or to diagnose diseased conditions including prostate cancer. In addition, these cDNA collections can be used in methods of designing novel therapeutic agents useful for treating prostate cancer. Methods of making these collections through subtraction hybridization are described.
摘要翻译：本发明鉴定富集前列腺体内平衡调节基因，前列腺退化以及程序性细胞死亡调节基因的cDNA集合。这些新颖的cDNA集合提供了编码蛋白质的cDNA，这些蛋白质是一般程序性细胞死亡或前列腺退化特有的蛋白质。这些转录物可用作筛选，监测和/或诊断包括前列腺癌在内的疾病状况的标记。此外，这些cDNA集合可用于设计用于治疗前列腺癌的新型治疗剂的方法。描述通过减法杂交进行这些收集的方法。

7. 发明申请

WO1997040155A1 PROTEIN MEDIATING NEURONAL-GLIAL INTERACTION, DNA ENCODING THE SAME, AND METHODS OF USE THEREOF 审中-公开
标题翻译：蛋白质介导神经 - 神经相互作用，编码其的DNA及其使用方法
公开(公告)号：WO1997040155A1
公开(公告)日：1997-10-30
申请号：PCT/US1997006415
申请日：1997-04-17
申请人： THE ROCKEFELLER UNIVERSITY
发明人： THE ROCKEFELLER UNIVERSITY , HEINTZ, Nathaniel , HATTEN, Mary, E.
IPC分类号： C12N15/12
CPC分类号： C07K14/705 , A61K38/00 , C12N2799/021
摘要： The present invention relates to a CNS neuronal antigen which functions in neuron-glia interaction key to CNS brain development, including glial-guided migration. This antigen acts as a novel signaling molecule that is expressed in newly generated neurons in the developing brain, and the deduced amino acid sequence of which reveals a novel secondary structure containing three EGF and two fibronectin type III repeats. The invention also relates to the nucleic acids encoding the neuronal antigen, and to antibodies directed to the antigen, and antisense nucleic acids and ribozymes directed to the nucleic acids. Also contemplated are diagnostic materials and therapeutic compositions, and corresponding methods, that may comprise or be derived from the nucleic acids, the antigen, and antibodies, antisense molecules and ribozymes directed thereto.
摘要翻译：本发明涉及CNS神经元抗原，其在CNS脑发育的神经元 - 神经胶质细胞相互作用关键中起作用，包括胶质细胞引导的迁移。该抗原作为在发育中的脑中新产生的神经元中表达的新型信号分子，其推导的氨基酸序列揭示了含有三种EGF和两种纤连蛋白III型重复序列的新二级结构。本发明还涉及编码神经元抗原的核酸，以及针对抗原的抗体以及指向核酸的反义核酸和核酶。还考虑了诊断材料和治疗组合物以及相应的方法，其可以包含或衍生自指导其的核酸，抗原和抗体，反义分子和核酶。

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