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1. 发明申请

WO2015143381A2 COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF 审中-公开
标题翻译：用于生产化学品及其衍生物的组合物和方法
公开(公告)号：WO2015143381A2
公开(公告)日：2015-09-24
申请号：PCT/US2015/021848
申请日：2015-03-20
申请人： SYNTHETIC GENOMICS, INC.
发明人： KAMBOURAKIS, Spiros , GRIFFIN, Benjamin, M. , MARTIN, Kevin, V.
IPC分类号： C12P7/58 , C12P17/04
CPC分类号： C12P7/58 , C12P7/44 , C12P17/06
摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.
摘要翻译：本发明提供了生产一种或多种酶途径的产物的方法。用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中L-赖康酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。所述方法包括作为步骤的酶和化学转化。还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。该方法还涉及使用以高特异性和高效率进行反应的工程化酶。可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。

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