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    • 4. 发明申请
    • METHOD FOR PRODUCING OPTICALLY ACTIVE CYANHYDRINS
    • 生产光学活性氰氢的方法
    • WO0138554A3
    • 2001-12-27
    • PCT/EP0011743
    • 2000-11-24
    • SUEDDEUTSCHE KALKSTICKSTOFFGROEGER HARALDVORLOP KLAUS DIETERCAPAN EMINE
    • GROEGER HARALDVORLOP KLAUS-DIETERCAPAN EMINE
    • C12N11/08C12N11/10C12P13/00C12N9/88C12N11/00
    • C12P13/004C12N11/08C12N11/10C12Y401/0201
    • The invention relates to a method for producing optically active cyanhydrins by reacting oxo compounds, such as for example aldehyde and/or ketone with hydrogen cyanide and/or with a cyanide donor in the presence of an inclusion compound which contains an oxynitrilase with an increased molecular weight. According to said method, an oxynitrilase is used, whose molecular weight is first increased by cross-linkage and which is subsequently converted into a gel inclusion compound and/or a gel complex compound, said conversions occurring substantially in two independent steps. The method which is carried out in particular at temperatures of between - 10 and + 30 DEG C and at pH values of between 3.0 and 6.0, preferably in an aqueous or hydrous system, but also in organic solvents, uses a component which contains enzymes. In said component, the molecular-weight of the enzyme has been increased, in particular by co-cross-linkage or by flocculation and said enzyme is used either in a free form or is enclosed by natural or artificial cells. A deciding factor in this method is the fact that the enzyme is enclosed by or complexed in a gel, which results in negligibly small attrition and leaching losses at a practically constant high level of enzyme activity, rendering the method extremely effective and economic.
    • 公开了一种用于被氧化合物的反应的光学活性氰醇,如,醛和/或酮与氢氰化物和/或与一个包含molekuargewichtsvergrösserte氰醇,其中一个使用了氰醇的包合化合物的存在下,氰化物供体的制备方法, 其已经通过交联在两个独立步骤中基本上分子扩大,并随后转化为凝胶夹杂物和/或凝胶复合物化合物。 的方法,特别是,在之间的温度 - 10执行和+ 30℃,并以3.0至6.0的pH值,优选在含水或含水的系统中,而且在有机溶剂中,使用的酶 含有组分,其中所述酶本身是特别通过共交联或通过絮凝molekulargewichtsvergrössert并用于包括以游离形式或以天然或人工细胞。 关键在这个过程是酶被封装在凝胶或络合,使磨损小和浸出损失可以忽略不计在几乎不变的高酶活性,这使得该方法非常有效和经济的事实。