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    • 4. 发明申请
    • METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM
    • 用于发酵微生物的方法,系统和组合物
    • WO2017112471A1
    • 2017-06-29
    • PCT/US2016/066469
    • 2016-12-14
    • SHELL OIL COMPANYSHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.
    • PARKER, David AlexanderZHANG, KunBOTELLA-FRANCO, CarolinaLIANG, YiZUROFF, Trevor Roman
    • C12N1/18C12N1/32C12N1/16
    • C12N1/16C12N1/18C12N1/32
    • Methods, systems, and compositions for propagating a fermentation microorganism that can have improved product yield during fermentation of a hydrolysate containing at least one growth inhibitor, including ethanol. In some embodiments, the method comprises (a) providing a generated according to a method comprising: - ensiling a cellulosic biomass material for at least 24 hours to generate ethanol in the cellulosic biomass material to generate an ensiled cellulosic biomass material; - pretreating the ensiled cellulosic biomass material with a solution comprising an alpha-hydroxysulfonic acid to produce a pretreated material comprising ethanol; and - introducing one or more cellulases to the pretreated material to produce the hydrolysate; (b) providing a propagation reactor containing: a propagation medium comprising: the hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L; and a first cell mass of a fermentation microorganism.
    • 用于繁殖发酵微生物的方法,系统和组合物,所述发酵微生物在含有至少一种生长抑制剂(包括乙醇)的水解产物的发酵期间可具有提高的产物产量。 在一些实施方案中,所述方法包括(a)提供根据以下方法产生的方法,所述方法包括: - 将纤维素类生物质材料保温至少24小时以在纤维素类生物质材料中产生乙醇以产生青贮的纤维素类生物质材料; - 用包含α-羟基磺酸的溶液预处理所述青贮的纤维素类生物质材料以产生包含乙醇的预处理材料; 和 - 将一种或多种纤维素酶引入预处理的材料以产生水解产物; (b)提供增殖反应器,所述增殖反应器包含:繁殖介质,其包含:至少25体积%的量的水解产物和大于14g / L的量的葡萄糖; 和发酵微生物的第一细胞团。