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1. 发明申请

WO2009151844A1 MULTIPOTENT STEM CELL CULTURES 审中-公开
标题翻译：多元干细胞培养
公开(公告)号：WO2009151844A1
公开(公告)日：2009-12-17
申请号：PCT/US2009/043426
申请日：2009-05-11
申请人： SAINT PETER'S COLLEGE , SCIORRA, Leonard
发明人： SCIORRA, Leonard
IPC分类号： C12N5/08 , C12N5/00
CPC分类号： C12N5/0662 , C12N5/0605 , C12N5/0607 , C12N2502/02 , C12N2506/1307
摘要： The invention provides methods for propagation of multipotent stem cells from human skin fibroblast samples using an appropriate medium, such as an amniotic fluid medium (AFM), and subsequent differentiation of the cells into cells of any of the three germ layers. The invention also provides methods of differentiating and making various tissues from multipotent cells in skin fibroblasts cultures that are capable of in vitro differentiation and that the cells are useful as a source of in vivo gene and/or autologist cell therapy. Isolated multipotent stem cells, culture of multipotent stem cells, and differentiated cells derived from the culture multipotent stem cells that are obtained by the methods disclosed herein also are provided. The methods, cells, cultures, media, banks, batches, and collections so provided can be used for various medical, research, diagnostic and therapeutic uses.
摘要翻译：本发明提供了使用适当培养基（例如羊水培养基（AFM））从人皮肤成纤维细胞样品繁殖多能干细胞的方法，以及随后将细胞分化成三个胚层中任何一个的细胞。本发明还提供了在皮肤成纤维细胞培养物中分化和制备来自多能细胞的各种组织的方法，其能够进行体外分化，并且该细胞可用作体内基因和/或自体细胞治疗的来源。还提供了通过本文公开的方法获得的分离的多能干细胞，多能干细胞的培养物和衍生自培养多潜能干细胞的分化细胞。如此提供的方法，细胞，培养物，媒体，银行，批次和收藏可用于各种医学，研究，诊断和治疗用途。

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