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2. 发明申请

WO2017142913A1 METHOD OF MAKING POLYNUCLEOTIDES USING AN ANION TOROIDAL VORTEX 审中-公开
标题翻译：使用阴离子环状涡旋制备多核苷酸的方法
公开(公告)号：WO2017142913A1
公开(公告)日：2017-08-24
申请号：PCT/US2017/017918
申请日：2017-02-15
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： CHURCH, George M. , LEE, Howon , PALLA, Mirko
IPC分类号： C07H21/04 , C07H19/00 , C12P19/30 , C12P19/34 , C12N9/12
CPC分类号： C07H21/04 , C12N9/1241 , C12P19/34
摘要： A method for making a polynucleotide is provided that includes (a) delivering one or more reaction reagents including an error prone or template independent DNA polymerase, cations and a selected nucleotide to a reaction site including an initiator sequence having a terminal nucleotide for a time period and under conditions sufficient to covalently add a desired number of the selected nucleotide to the terminal nucleotide at the 3' end of the initiator such that the selected nucleotide becomes a terminal nucleotide, moving cations away from the initiator sequence using an anion toroidal vortex to inhibit covalent addition of the selected nucleotide by the error prone or template independent DNA polymerase, removing the reaction reagents from the reaction site, and (b) repeating step (a) until the polynucleotide is formed.
摘要翻译：提供了制备多核苷酸的方法，其包括（a）将一种或多种包括易错或模板独立的DNA聚合酶，阳离子和选择的核苷酸的反应试剂递送至包含引发剂序列的反应位点具有末端核苷酸一段时间并在足以将所需数量的所选核苷酸共价添加至引发剂3'末端的末端核苷酸的条件下，使得所选核苷酸成为末端核苷酸，将阳离子移离引发剂使用阴离子环形涡旋序列来抑制通过易错或模板独立的DNA聚合酶共价加入所选核苷酸，从反应位点除去反应试剂，和（b）重复步骤（a）直至形成多核苷酸。 p>

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