专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2011142529A3 SOLUBLE EXPRESSION OF BULKY FOLDED ACTIVE PROTEINS 审中-公开
标题翻译：大肠杆菌折叠活性蛋白的可溶性表达
公开(公告)号：WO2011142529A3
公开(公告)日：2012-04-26
申请号：PCT/KR2011001465
申请日：2011-03-03
申请人： REPUBLIC OF KOREA REPRESENTED BY NAT FISHERIES RES & DEV INST , LEE SANG JUN , KIM YOUNG OK , NAM BO HYE , KONG HEE JEONG , KIM KYUNG KIL
发明人： LEE SANG JUN , KIM YOUNG OK , NAM BO HYE , KONG HEE JEONG , KIM KYUNG KIL
IPC分类号： C12N15/63 , C12N15/11
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/50 , C12N15/70 , C12P21/06
摘要： The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.
摘要翻译：本发明涉及通过将前导肽与酸性或碱性pI连接来增强异源蛋白质，特别是具有一个或多个跨膜样结构域或分子内二硫键的大体积折叠活性异源蛋白质的可溶性表达和分泌的表达载体和方法并具有高亲水性; 通过用具有酸性或中性pI和高亲水性的异源蛋白的N-末端取代一个或多个氨基酸; 或降低编码具有碱性pI值和高亲水性的前导肽的多核苷酸的升高GRNA值。表达载体和方法可以用于产生异源蛋白质并通过防止不溶性包涵体的形成和通过增强异源蛋白质分泌到周质或外部细胞中的分泌效率来转导患者中的治疗性蛋白质。

2. 发明申请

WO2007089093A1 PRODUCTION OF A SOLUBLE NATIVE FORM OF RECOMBINANT PROTEIN BY THE SIGNAL SEQUENCE AND SECRETIONAL ENHANCER 审中-公开
标题翻译：通过信号序列和分泌增强剂生产重组蛋白的可溶性本体
公开(公告)号：WO2007089093A1
公开(公告)日：2007-08-09
申请号：PCT/KR2007000515
申请日：2007-01-30
申请人： REPUBLIC OF KOREA REPUBLIC OF , LEE SANG JUN , KIM YOUNG OK , NAM BO-HYE
发明人： LEE SANG JUN , KIM YOUNG OK , NAM BO-HYE
IPC分类号： C12N15/63
CPC分类号： C12N15/63 , C07K14/43509 , C07K14/461 , C07K2319/02 , C07K2319/034 , C07K2319/50 , C12P21/02
摘要： The present invention is drawn to a method for enhancing secretional efficiency of a heterologous protein using a secretional enhancer consisting of a modified signal sequence which comprises the N-region of a signal sequence and/or a hydrophobic fragment of the said signal sequence comprising the said N-region and/or the hydrophilic polypeptide. The method of the present invention can be used not only for production of recombinant heterologous proteins by inhibiting insoluble precipitation and enhancing secretional efficiency of the recombinant protein into the periplasm or the extracellular fluid and but also for transduction of therapeutic proteins by enhancing membrane-permeability of the recombinant protein using a strong secretional enhancer.
摘要翻译：本发明涉及一种用于增强异源蛋白质的分泌效率的方法，该方法使用由修饰的信号序列组成的分泌增强子，所述修饰的信号序列包含所述信号序列的N区和/或所述信号序列的疏水片段，所述信号序列包含所述 N-区和/或亲水性多肽。本发明的方法不仅可用于通过抑制不溶性沉淀和提高重组蛋白进入周质或细胞外液的分泌效率而且通过增强膜的渗透性来转导治疗性蛋白质来生产重组异源蛋白该重组蛋白使用强分泌增强子。

3. 发明申请

WO2011002174A3 SIGNAL-PROCESSING METHOD FOR DETECTING A QUENCH OF A SUPERCONDUCTING MAGNETIC OPERATING CURRENT 审中-公开
标题翻译：用于检测超导磁性工作电流的信号的信号处理方法
公开(公告)号：WO2011002174A3
公开(公告)日：2011-03-31
申请号：PCT/KR2010004082
申请日：2010-06-23
申请人： KOREA BASIC SCIENCE INST , PARK KAP RAE , CHU YONG , KIM YOUNG OK , YONEKAWA HIROFUMI
发明人： PARK KAP RAE , CHU YONG , KIM YOUNG OK , YONEKAWA HIROFUMI
IPC分类号： G01R19/255 , G01R19/25
CPC分类号： H02H7/001 , Y02E40/68
摘要： The present invention relates to a signal-processing method for detecting a quench of a superconducting magnetic operating current. A quench-detecting apparatus for detecting a quench of a superconducting coil comprises: measuring means installed on a normal busbar which supplies current to the superconducting coil to measure the current of the superconducting coil; and processing means for interpreting the measurement value obtained by the measuring means through a mathematical algorithm to output a measurement value. The signal-processing method for outputting a measurement value from the processing means and detecting a quench of a tokamak system comprises: a step of removing a voltage offset from the measurement value of the secondary voltage detected by the measuring means; a step of removing the voltage noise of the second voltage using an integration method; and a step of removing the drift of a minute voltage for the voltage waveform of the secondary voltage.
摘要翻译：本发明涉及一种用于检测超导磁操作电流的骤冷的信号处理方法。用于检测超导线圈的骤冷的淬火检测装置包括：安装在正常母线上的测量装置，其向超导线圈提供电流以测量超导线圈的电流; 以及用于通过数学算法解释由测量装置获得的测量值以输出测量值的处理装置。用于从处理装置输出测量值并检测托卡马克系统的淬火的信号处理方法包括：从测量装置检测到的次级电压的测量值中去除电压偏移的步骤; 使用积分方法去除第二电压的电压噪声的步骤; 以及去除次级电压的电压波形的微小电压漂移的步骤。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式