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1. 发明申请

WO2012138420A3 SYSTEMS AND METHODS FOR HARVESTING TARGET PARTICLES OF A SUSPENSION 审中-公开
公开(公告)号：WO2012138420A3
公开(公告)日：2012-10-11
申请号：PCT/US2012/025113
申请日：2012-02-14
申请人： RARECYTE, INC. , SEUBERT, Ronald, C. , GOODWIN, Paul, C. , STILLWELL, Jackie, L.
发明人： SEUBERT, Ronald, C. , GOODWIN, Paul, C. , STILLWELL, Jackie, L.
IPC分类号： A61M1/02 , B04B7/08 , B04B1/00 , C12M1/10 , C12M1/24
摘要： Tube and float systems and methods for isolating, enumerating, and harvesting target materials of a suspension are described. In one aspect, a tube and float system includes a filter embedded in a tube cap. The filter enables the passage of fluids but prevents the passage of the target materials. The tube and float system can be used to isolate and enumerate the target materials by centrifuging the tube and float system with the suspension to trap the target materials between the float and inner wall of the tube. Fluids above and below the float are poured off and a second fluid can be introduced to the tube to re-suspend the trapped target material. The second fluid can be poured through the filter in the cap to trap the target material against the filter. The target material can be enumerated and analyzed.

2. 发明申请

WO2011050366A2 METHODS FOR CHANGING DENSITIES ON NON-TARGET PARTICLES OF A SUSPENSION 审中-公开
标题翻译：改变悬浮剂非目标颗粒浓度的方法
公开(公告)号：WO2011050366A2
公开(公告)日：2011-04-28
申请号：PCT/US2010/053978
申请日：2010-10-25
申请人： RARECYTE, INC. , GOODWIN, Paul, C.
发明人： GOODWIN, Paul, C.
IPC分类号： C12Q1/24 , G01N33/48 , C12M1/24 , G01N15/05 , G01N5/00 , C12M1/42
CPC分类号： G01N1/4077 , G01N2001/4083 , Y10T436/255
摘要： This disclosure is directed to methods for isolating target materials from non-target materials of a suspension that have a similar density to that of the target material. A suspension suspected of containing a target material is added to a tube and float system. A solution containing molecules that interact specifically with the non-target materials to change the density of the non-target materials in also added. A float is also added to the tube, and the tube, float, and suspension are centrifuged together. The float has a specific gravity that positions the float at approximately the same level as a layer containing the target material when the tube, float and suspension are centrifuged. During centrifugation, the non-target material/molecules complexes are drawn either below the float or above float, leaving the target material between the outer surface of the float and the inner surface of the tube.
摘要翻译：本公开涉及从具有与靶材料密度相似的密度的悬浮液的非目标材料中分离目标材料的方法。将疑似含有目标材料的悬浮液加入到管和浮子系统中。含有与非目标材料特异性相互作用的分子的溶液，以改变非目标材料的密度。还将一个浮子加入管中，将管，浮子和悬浮液一起离心。当离心管，浮子和悬浮液时，浮子具有将浮子定位在与包含目标材料的层大致相同的水平处的比重。在离心过程中，非目标材料/分子复合物被绘制在浮子或漂浮浮选下方，留下浮体外表面和管内表面之间的目标材料。

3. 发明申请

WO2011087632A3 SYSTEM AND METHOD FOR DENSE-STOCHASTIC-SAMPLING IMAGING 审中-公开
公开(公告)号：WO2011087632A3
公开(公告)日：2011-07-21
申请号：PCT/US2010/059447
申请日：2010-12-08
申请人： APPLIED PRECISION, INC. , DANUSER, Gaudenz , GOODWIN, Paul, C.
发明人： DANUSER, Gaudenz , GOODWIN, Paul, C.
IPC分类号： G01N21/64 , G06T1/00
摘要： Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images.

4. 发明申请

WO2004083832A1 MULTI-AXIS INTEGRATION SYSTEM AND METHOD 审中-公开
标题翻译：多轴积分系统和方法
公开(公告)号：WO2004083832A1
公开(公告)日：2004-09-30
申请号：PCT/US2004/007323
申请日：2004-03-12
申请人： APPLIED PRECISION, LLC , REESE, Steven, A. , BROWN, Carl, S. , GOODWIN, Paul, C.
发明人： REESE, Steven, A. , BROWN, Carl, S. , GOODWIN, Paul, C.
IPC分类号： G01N21/25
CPC分类号： G02B21/367 , G06T1/0007 , H04N5/349 , H04N5/37206 , H04N5/3743
摘要： An image acquisition system and method employing multi-axis integration (MAI) may incorporate both optical axis integration (OAI) and time-delay integration (TDI) techniques. Disclosed MAI systems and methods may integrate image data in the z direction as the data are acquired, projecting the image data prior to deconvolution. Lateral translation of the image plane during the scan in the z direction may allow large areas to be imaged in a single scan sequence.
摘要翻译：使用多轴积分（MAI）的图像采集系统和方法可以并入光轴积分（OAI）和时延整合（TDI）技术。公开的MAI系统和方法可以在采集数据时将图像数据集中在z方向上，在去卷积之前投影图像数据。在z方向扫描期间的图像平面的横向平移可允许以单个扫描序列成像大面积。

5. 发明申请

WO2004061514A2 WAVELENTH-SPECIFIC PHASE MICROSCOPY 审中-公开
标题翻译：波形特异相显微镜
公开(公告)号：WO2004061514A2
公开(公告)日：2004-07-22
申请号：PCT/US2003/041455
申请日：2003-12-23
申请人： APPLIED PRECISION, LLC , GOODWIN, Paul, C.
发明人： GOODWIN, Paul, C.
IPC分类号： G02B21/00
CPC分类号： G02B21/14
摘要： A system and method of generating and acquiring phase contrast microscope images without interfering with the intensity and optical quality of other microscopy modalities employ wavelength-specific illumination and attenuation strategies for phase microscopy applications. A wavelength-specific objective phase ring that is opaque only at specific wavelengths may be used in conjunction with a phase microscopy apparatus. Attenuated wavelengths may be controlled such that opacity may be selectively provided only with respect to wavelengths that are outside of the desired range for the fluorescence signals being monitored. Illumination within the opaque wavelength range for the objective phase ring may be selected for phase microscopy applications. Accordingly, an objective phase ring effective for enabling wavelength-specific phase microscopy may not interfere with normal usage of the microscope for other applications such as, for example, fluorescence microscopy.
摘要翻译：生成和获取相差显微镜图像而不干扰其他显微镜模式的强度和光学质量的系统和方法使用相位显微镜应用的波长特异性照明和衰减策略。仅在特定波长下不透明的波长特异性目标相环可以与相位显微镜装置结合使用。可以控制衰减波长，使得不透明度可以仅针对被监视的荧光信号的期望范围外的波长选择性地提供。对于相位显微镜应用，可以选择目标相位环的不透明波长范围内的照明。因此，对于实现波长特异性相位显微镜有效的客观相位环可能不会影响显微镜对于例如荧光显微镜的其他应用的正常使用。

6. 发明申请

WO2004061435A1 SYSTEM AND METHOD OF ILLUMINATING LIVING CELLS FOR IMAGING 审中-公开
标题翻译：用于成像的照射生活细胞的系统和方法
公开(公告)号：WO2004061435A1
公开(公告)日：2004-07-22
申请号：PCT/US2003/041042
申请日：2003-12-23
申请人： APPLIED PRECISION, LLC , GOODWIN, Paul, C.
发明人： GOODWIN, Paul, C.
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G02B21/16
摘要： A system and method of providing illumination for a live-cell imaging system deliver modulated light for illumination of a sample. Modulation of illumination intensity may be effectuated by pulsing the output of a light source, for example, or by selectively interrupting light provided by the source. Such modulated illumination may have particular utility in applications where reducing the number of electrons in a super-excited state may minimize the rate of photobleaching and photodamage in the molecules being studied.
摘要翻译：为活细胞成像系统提供照明的系统和方法提供用于照射样品的调制光。照明强度的调制可以通过例如脉冲输出光源或者通过选择性地中断源提供的光来实现。这种调制照明可以在减少超激发态的电子数量的应用中具有特殊的用途，这可能使正在研究的分子中的光漂白和光损伤的速率最小化。

7. 发明申请

WO2009036192A1 METHOD OF DISPERSING IMMERSION LIQUID ON A SPECIMEN SUBSTRATE FOR HIGH RESOLUTION IMAGING AND LITHOGRAPHIE 审中-公开
标题翻译：在高分辨率成像和光刻技术的样品基板上分散浸没液的方法
公开(公告)号：WO2009036192A1
公开(公告)日：2009-03-19
申请号：PCT/US2008/076048
申请日：2008-09-11
申请人： APPLIED PRECISION, INC. , DOUGHERTY, William , GOODWIN, Paul, C. , REESE, Steven, A. , STEWART, David
发明人： DOUGHERTY, William , GOODWIN, Paul, C. , REESE, Steven, A. , STEWART, David
IPC分类号： G02B21/33
CPC分类号： G02B21/33 , G02B21/002 , G02B21/02 , G03F7/70341
摘要： Methods and apparatus are described for delivering index-matching immersion liquid in high numerical-aperture optical microscopy and lithography. An array of immersion liquid droplets (70) is delivered to a specimen substrate or specimen substrate cover by an immersion liquid printing apparatus. An immersion liquid reservoir provides immersion liquid to the printer by a precision pump The printer delivers immersion liquid to the substrate or substrate cover in arrays of immersion liquid droplets of defined volumes and array patterns. The volumes and patterns of array droplets delivered to the substrate or substrate cover are optimized to maintain adequate immersion liquid between the substrate or substrate cover and an immersion objective while avoiding the formation of air bubbles in the immersion liquid and the accumulation of excess volumes of immersion liquid.
摘要翻译：描述了用于在高数值孔径光学显微镜和光刻中输送折射率匹配浸液的方法和装置。浸没液滴（70）的阵列通过浸没液体印刷装置输送到样品基片或样品基片盖。浸没液体储存器通过精密泵将浸入液体提供给打印机。打印机将浸没液体以定义的体积和阵列图案的浸没液滴的阵列输送到基底或基底盖。优化输送到衬底或衬底盖的阵列液滴的体积和图案，以在衬底或衬底盖和浸没物体之间保持足够的浸没液体，同时避免在浸液中形成气泡和积累过量的浸液液体。

8. 发明申请

WO2004113922A3 SYSTEM AND METHOD EMPLOYING PHOTOKINETIC TECHNIQUES IN CELL BIOLOGY IMAGING APPLICATIONS 审中-公开
公开(公告)号：WO2004113922A3
公开(公告)日：2004-12-29
申请号：PCT/US2004/019452
申请日：2004-06-18
申请人： APPLIED PRECISION, LLC , GOODWIN, Paul, C. , BROWN, Carl, S. , REESE, Steven, A.
发明人： GOODWIN, Paul, C. , BROWN, Carl, S. , REESE, Steven, A.
IPC分类号： G01N33/58
摘要： A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

9. 发明申请

WO2013032393A1 SYSTEM AND METHOD FOR CONTINUOUS, ASYNCHRONOUS AUTOFOCUS OF OPTICAL INSTRUMENTS USING MULTIPLE CALIBRATION CURVES 审中-公开
标题翻译：使用多个校准曲线的连续光学仪器的异步自适应系统和方法
公开(公告)号：WO2013032393A1
公开(公告)日：2013-03-07
申请号：PCT/SE2012/050914
申请日：2012-08-29
申请人： APPLIED PRESICION, INC. , GE HEALTHCARE BIO-SCIENCES AB , COOPER, Jeremy, R. , GOODWIN, Paul, C.
发明人： COOPER, Jeremy, R. , GOODWIN, Paul, C.
IPC分类号： G02B21/16 , G02B7/09 , G03B3/02
CPC分类号： G02B21/16 , G02B21/245
摘要： The current application is directed to autofocus subsystems within optical instruments that continuously monitor the focus of the optical instruments and adjust distances within the optical instrument along the optical axis in order to maintain a precise and stable optical-instrument focus at a particular point or surface on, within, or near a sample. Certain autofocus implementations operate asynchronously with respect to operation of other components and subsystems of the optical instrument in which they are embedded. The described autofocus subsystems employ multiple calibration curves to precisely adjust the z-position of an optical instrument.
摘要翻译：目前的应用是针对光学仪器内的自动对焦子系统，其连续地监视光学仪器的焦点并且沿着光轴调整光学仪器内的距离，以便在特定点或表面上保持精确和稳定的光学仪器焦点，在样本内或附近。某些自动对焦实现方式相对于嵌入其中的光学仪器的其他组件和子系统的操作而异步操作。所描述的自动对焦子系统使用多个校准曲线来精确地调整光学仪器的z位置。

10. 发明申请

WO2012096619A1 SYSTEMS AND METHODS FOR CAMERA-BASED IMAGE PROCESSING IN MICROSCOPY INSTRUMENTS 审中-公开
标题翻译：用于显微镜仪器中基于摄像机的图像处理的系统和方法
公开(公告)号：WO2012096619A1
公开(公告)日：2012-07-19
申请号：PCT/SE2012/050011
申请日：2012-01-11
申请人： APPLIED PRECISION, Inc. , GE HEALTHCARE BIO-SCIENCES AB , GOODWIN, Paul, C.
发明人： GOODWIN, Paul, C.
IPC分类号： G01N21/64 , G01B21/06
CPC分类号： G01N21/6458 , G01B11/002 , G01N21/6408 , G01N21/6428 , G01N2201/06113 , G01N2201/12 , G02B21/02 , G02B21/06 , G02B21/082 , G02B21/16 , G02B21/361 , G02B21/367 , G06T3/4053
摘要： Systems and methods for executing super-resolution microscopy of a specimen with most of the image processing performed in a camera of a fluorescence microscopy instrument are described. In one aspect, the camera includes one or more processors to execute machine-readable instructions that control excitation light output from a multi-channellight source, control capture of intermediate images of the specimen, and perform image processing of the intermediate images to produce a final super-resolution image of the specimen.
摘要翻译：描述了在荧光显微镜仪器的照相机中执行的大部分图像处理用于执行超分辨率显微镜检查的系统和方法。在一个方面，相机包括一个或多个处理器，用于执行机器可读指令，其控制从多通道光源输出的激发光，控制样本的中间图像的拍摄，并执行中间图像的图像处理以产生最终标本的超分辨率图像。

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