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1. 发明申请

WO2009072705A1 METHOD FOR DETECTING NUCLEIC ACIDS BY SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE 审中-公开
标题翻译：通过同位素放射核酸和信号探针检测核酸的方法
公开(公告)号：WO2009072705A1
公开(公告)日：2009-06-11
申请号：PCT/KR2008/002341
申请日：2008-04-24
申请人： RAPLEGENE INC. , KIM, Min Hwan , LEE, Sook , KIM, Un Ok , JEONG, Ji Won , LEE, Joo Hee
发明人： KIM, Min Hwan , LEE, Sook , KIM, Un Ok , JEONG, Ji Won , LEE, Joo Hee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2525/161
摘要： The present invention relates to a method for detecting target nucleic acids by simultaneous isothermal amplification of the target nucleic acids and a signal probe 5 using an external primer set, a DNA-RNA-DNA hybrid primer set and a DNA- RNA-DNA hybrid signal probe. The method according to the present invention can be used to amplify target nucleic acids in a sample, rapid and exact manner without the risk of contamination compared to the conventional methods such as PCR, and it can simultaneously amplify target nucleic acid and a signal probe, so that it can 0 be applied to various genome projects, detection and identification of a pathogen, detection of gene modification producing a predetermined phenotype, detection of hereditary diseases or determination of sensibility to diseases, and estimation of gene expression. Thus, it is useful for molecular biological studies and disease diagnosis.
摘要翻译：本发明涉及通过使用外部引物组，DNA-RNA-DNA杂交引物组和DNA-RNA-DNA混合信号同时等温扩增靶核酸和信号探针5来检测靶核酸的方法探测。根据本发明的方法可以用于与常规方法如PCR相比以快速和准确的方式扩增靶核酸而没有污染的风险，并且可以同时扩增靶核酸和信号探针，从而可以将其应用于各种基因组项目，病原体的检测和鉴定，产生预定表型的基因修饰的检测，遗传性疾病的检测或疾病的敏感性的测定以及基因表达的估计。因此，它对分子生物学研究和疾病诊断有用。

2. 发明申请

WO2007043751A1 METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND METHOD FOR DETECTING NUCLEIC ACIDS USING SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE 审中-公开
标题翻译：用于核酸的同位素放大的方法和使用核酸和信号探针的同时同位素放大来检测核酸的方法
公开(公告)号：WO2007043751A1
公开(公告)日：2007-04-19
申请号：PCT/KR2006/003577
申请日：2006-09-08
申请人： RAPLEGENE INC. , KIM, Min Hwan , JEONG, Ji Won , KIM, Un Ok
发明人： KIM, Min Hwan , JEONG, Ji Won , KIM, Un Ok
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6846 , C12Q1/6865 , C12Q2537/143 , C12Q2537/1376 , C12Q2521/101
摘要： The present invention relates to a method for isothermal amplification of nucleic acids and a method for detecting nucleic acids, which comprises characterized in simultaneous isothermal amplification of nucleic acids and a signal probe to a method for isothermal amplification of target nucleic acids using an external primer set and RNA/DNA hybrid primer set, and a method for detecting amplification products by amplifying nucleic acids and a signal probe simultaneously using an external primer set, RNA-DNA hybrid primer set and DNA-RNA-DNA hybrid probe. The method according to the present invention is convenient compared with the conventional method, it is possible to amplify the target nucleic acids rapidly and exactly without a risk of contamination, and it can simultaneously amplify a signal probe, so that it can be applied to various genome project, such as detection and identification of a pathogen, detection of gene modification causing predetermined phenotype, detection of hereditary diseases or determination of sensibility to diseases, estimation of gene expression and apply to genome project, thus being useful for molecular biological studies and disease diagnosis.
摘要翻译：本发明涉及核酸的等温扩增方法和检测核酸的方法，其特征在于将核酸同时等温扩增和信号探针用于使用外部引物组等位扩增靶核酸的方法和RNA / DNA杂交引物组，以及通过使用外部引物组，RNA-DNA杂交引物组和DNA-RNA-DNA杂交探针同时扩增核酸和信号探针来检测扩增产物的方法。根据本发明的方法与常规方法相比是方便的，可以快速准确地扩增靶核酸而没有污染的风险，并且可以同时放大信号探针，从而可以应用于各种基因组计划，如病原体的检测和鉴定，检测导致预期表型的基因修饰，遗传性疾病的检测或疾病敏感性的测定，基因表达的估计和应用于基因组计划，因此可用于分子生物学研究和疾病诊断。

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