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1. 发明申请

WO2010062518A1 SULFONYLUREA-RESPONSIVE REPRESSOR PROTEINS 审中-公开
标题翻译： SULFONYLUREA-RESPONSOR REPRESSOR蛋白质
公开(公告)号：WO2010062518A1
公开(公告)日：2010-06-03
申请号：PCT/US2009/061661
申请日：2009-10-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , LASSNER, Michael , LOOGER, Loren L. , MCBRIDE, Kevin E. , MCGONIGLE, Brian
发明人： LASSNER, Michael , LOOGER, Loren L. , MCBRIDE, Kevin E. , MCGONIGLE, Brian
IPC分类号： C07K14/195
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译：提供了与使用磺酰脲类阻遏物相关的组合物和方法。组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞以及包括多核苷酸的植物和种子的真核生物，和/或通过该方法产生的。还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。

2. 发明申请

WO2009091860A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：从家禽APHIDIDA中抑制目标多核苷酸的组合物和方法
公开(公告)号：WO2009091860A2
公开(公告)日：2009-07-23
申请号：PCT/US2009/031053
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件在被害虫摄入时，例如来自蚜科的害虫中，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了用于特异性蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列的表达减少对害虫的控制（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是蚜虫甘氨酸。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

3. 发明申请

WO2009091864A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开
标题翻译：用于抑制来自LYGUS的目标多核苷酸的组合物和方法
公开(公告)号：WO2009091864A2
公开(公告)日：2009-07-23
申请号：PCT/US2009/031057
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件被害虫摄取时，例如来自莱古氏属属的害虫，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了本文公开的来自特定多肽家族的多种靶多核苷酸，并进一步提供了SEQ ID NO：1-21所示的靶多核苷酸或其活性变体，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，有害生物是黄瓜（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

4. 发明申请

WO2012129373A2 METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS 审中-公开
标题翻译：用于生产复合转基因位置的方法
公开(公告)号：WO2012129373A2
公开(公告)日：2012-09-27
申请号：PCT/US2012/030061
申请日：2012-03-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , LASSNER, Michael , JANTZ, Derek , SMITH, James, Jefferson , CIGAN, Mark , FALCO, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-bin , SVITASHEV, Sergei
发明人： LASSNER, Michael , JANTZ, Derek , SMITH, James, Jefferson , CIGAN, Mark , FALCO, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-bin , SVITASHEV, Sergei
IPC分类号： C12N15/82 , A01H1/06 , A01H5/10 , C12N15/55
CPC分类号： C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
摘要翻译：公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。还公开了植物中的复杂的转基因性状基因座。复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

5. 发明申请

WO2005123929A2 PLASTID TRANSIT PEPTIDES 审中-公开
标题翻译：塑料过滤胶
公开(公告)号：WO2005123929A2
公开(公告)日：2005-12-29
申请号：PCT/US2005/020455
申请日：2005-06-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LASSNER, Michael , WILKINSON, Jack, Q.
发明人： LASSNER, Michael , WILKINSON, Jack, Q.
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C07K14/00 , C12N15/8221
摘要： The present invention provides novel plastid transit peptides that direct lacalization of attached moieties (e.g., polypeptides) into plant plastids. The present invension also relates to methods and compositions for lacalizing polypeptides to plant plastids including, but not limited to, transgenic plant production.
摘要翻译：本发明提供了将附着部分（例如，多肽）引导到植物质体中的新型质体转运肽。本发明还涉及用于将多肽拉染植物质体的方法和组合物，包括但不限于转基因植物生产。

6. 发明申请

WO2009124282A1 METHODS AND COMPOSITIONS FOR POLLINATION DISRUPTION 审中-公开
标题翻译：用于污染物破坏的方法和组合物
公开(公告)号：WO2009124282A1
公开(公告)日：2009-10-08
申请号：PCT/US2009/039528
申请日：2009-04-03
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ZHAO, Zuo-YU , WU, Yongzhong , LASSNER, Michael , HERSHEY, Howard, P.
发明人： ZHAO, Zuo-YU , WU, Yongzhong , LASSNER, Michael , HERSHEY, Howard, P.
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8216
摘要： Pollination process is disrupted by use of a pollination-disruption polynucleotide that renders the pollen unable to fertilize a sexually compatible ovule. The non-lethal nature of the pollen disruption polynucleotide is advantageous, particularly when operably linked to a transgenic polynucleotide of interest and prevents transmission of the polynucleotide through pollen. non-lethal markers are employed in an embodiment in which transgenic and non-transgenic seed can be sorted. A recombinase excision system is employed in an embodiment to activate the pollen disruption polynucleotide.
摘要翻译：通过使用授粉破坏多核苷酸破坏授粉过程，使得花粉不能施用性别相容的胚珠。花粉破坏多核苷酸的非致死性质是有利的，特别是当与感兴趣的转基因多核苷酸可操作地连接并且防止多核苷酸通过花粉传播时。在其中可以分选转基因和非转基因种子的实施方案中采用非致死性标记。在一个实施方案中采用重组酶切除系统来活化花粉破坏多核苷酸。

7. 发明申请

WO2006091219A2 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI 审中-公开
标题翻译：从病原菌保护植物的方法
公开(公告)号：WO2006091219A2
公开(公告)日：2006-08-31
申请号：PCT/US2005/023327
申请日：2005-06-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LASSNER, Michael , WU, Gusui , MULLER, Mathias L. , KEENAN, Robert J. , ALI, Hana
发明人： LASSNER, Michael , WU, Gusui , MULLER, Mathias L. , KEENAN, Robert J. , ALI, Hana
CPC分类号： C12N15/8282
摘要： Methods for protecting a plant from a plant pathogenic fungus are provided. A method for enhancing fungal pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antifungal polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antifungal polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受植物致病真菌的方法。还提供了使用本文公开的核苷酸序列增强植物中真菌病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗真菌多肽的核苷酸序列的启动子的表达盒。还公开了包含编码实施方案的抗真菌多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

8. 发明申请

WO2009091863A1 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES 审中-公开
标题翻译：用于抑制目标多核苷酸的组合物和方法
公开(公告)号：WO2009091863A1
公开(公告)日：2009-07-23
申请号：PCT/US2009/031056
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LASSNER, Michael
发明人： LASSNER, Michael
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C12N15/8218 , Y02A40/162
摘要： Methods and compositions which increase the concentration of an inhibitory RNA specific for a target sequence in a cell are provided. In one embodiment, the methods and compositions employ a first polynucleotide comprising a silencing element for a target pest sequence operably linked to a promoter active in the plant cell; and, a second polynucleotide comprising a suppressor enhancer element comprising the target pest sequence or an active fragment or variant thereof operably linked to a promoter active in the plant cell. The combined expression of the silencing element with the target pest sequence, or an active variant or fragment thereof, leads to the amplification of the inhibitory RNA produced from the silencing element over the achievable with only the expression of the silencing element alone. Thus, the various methods and compositions of the invention provide improved methods for the delivery of inhibitory RNA to a target organism.
摘要翻译：提供增加细胞中靶序列特异性抑制性RNA浓度的方法和组合物。在一个实施方案中，所述方法和组合物采用第一多核苷酸，其包含可操作地连接于在植物细胞中有活性的启动子的靶害虫序列的沉默元件; 以及第二个多核苷酸，其包含抑制增强子元件，其包含目标有害生物序列或与植物细胞中活性的启动子有效连接的活性片段或变体。沉默元件与目标害虫序列或其活性变体或片段的组合表达导致仅通过沉默元件的表达可实现的从沉默元件产生的抑制性RNA的扩增。因此，本发明的各种方法和组合物提供了将抑制性RNA递送至靶生物体的改进方法。

9. 发明申请

WO2009091862A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
公开(公告)号：WO2009091862A3
公开(公告)日：2009-07-23
申请号：PCT/US2009/031055
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

10. 发明申请

WO2006085966A2 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI AND NEMATODES 审中-公开
标题翻译：从病原真菌和NEMATODES保护植物的方法
公开(公告)号：WO2006085966A2
公开(公告)日：2006-08-17
申请号：PCT/US2005/023325
申请日：2005-06-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LASSNER, Michael , WU, Gusui , MULLER, Mathias L. , KEENAN, Robert J. , ALI, Hana , SHAH, Gowri , WEI, Jun-Zhi
发明人： LASSNER, Michael , WU, Gusui , MULLER, Mathias L. , KEENAN, Robert J. , ALI, Hana , SHAH, Gowri , WEI, Jun-Zhi
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受病原体，特别是病原真菌或线虫的方法。还提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

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