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    • 2. 发明申请
    • HIGH THROUGHPUT, SEED SAMPLING AND COLLECTION SYSTEM AND METHOD
    • 高通量,种子采样和收集系统及方法
    • WO2010108082A1
    • 2010-09-23
    • PCT/US2010/027948
    • 2010-03-19
    • PIONEER HI-BRED INTERNATIONAL, INC.COPE, Jason, M.JAEHNEL, Gary, L.MONGAN, Joshua, L.
    • COPE, Jason, M.JAEHNEL, Gary, L.MONGAN, Joshua, L.
    • G01N1/28G01N35/00G01N33/02
    • G01N1/286G01N35/00G01N2001/2866
    • A system and method for preparing a sample of seeds or representative seed portions are provided. In various embodiments, the system and method include a force applying member and a seed container that includes at least one compartment containing a seed. The force applying member is configured to apply a force to the seed so as to break the seed into two or more seed particles, which in some embodiments may be collected in a seed particle collector. The present invention improves on the prior art by greatly reducing (and in some embodiments eliminating) the manual processes typically involved in generating tissue samples from seeds and preparing the tissue for genetic analysis. Additionally, the present invention is scaleable, and can be configured to generate samples from many seeds in a short period of time. The present invention also minimizes the risk of contamination and cross-contamination of the seed particles.
    • 提供了一种用于制备种子样品或代表种子部分的系统和方法。 在各种实施例中,系统和方法包括施力构件和种子容器,其包括至少一个包含种子的隔室。 力施加构件构造成向种子施加力,以便将种子分解成两个或更多种子颗粒,其在一些实施方案中可以收集在种子颗粒收集器中。 本发明通过大大减少(并且在一些实施例中消除)通常涉及从种子产生组织样品并制备用于遗传分析的组织的手工过程来改进现有技术。 此外,本发明是可扩展的,并且可以被配置为在短时间内从许多种子生成样本。 本发明还使种子颗粒的污染和交叉污染的风险最小化。