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    • 4. 发明申请
    • MAIZE CYCLO1 GENE AND PROMOTER
    • MAIZE CYCLO1基因和促进剂
    • WO2006076189A3
    • 2007-02-22
    • PCT/US2006000153
    • 2006-01-06
    • PIONEER HI BRED INTDU PONTDIEHN SCOTTLU ALBERT LSIMS LYNNE EWARD KIM R
    • DIEHN SCOTTLU ALBERT LSIMS LYNNE EWARD KIM R
    • C07K14/415A01H5/00C12N15/82
    • C12N15/8227C07K14/415C12N15/8279
    • The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
    • 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码Cyclo1的基因的根优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中,并再生表达核苷酸序列的稳定转化的植物。 本发明还涉及编码植物环肽的分离的核酸。 本发明涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因,其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环肽。
    • 8. 发明申请
    • COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS
    • 组合物及其控制方法
    • WO2014153254A2
    • 2014-09-25
    • PCT/US2014029805
    • 2014-03-14
    • PIONEER HI BRED INTDU PONT
    • HU XUPRESNAIL JAMESRICHTMAN NINADIEHN SCOTTVAN ALLEN MICHELLEPROCYK LISA
    • C12N15/8218C12N15/8286Y02A40/162
    • Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
    • 提供了使用沉积元件的方法和组合物,当由有害生物摄入,例如鞘翅目植物害虫或双壳花属植物害虫时,降低害虫中靶序列的表达。 本发明提供了本文所公开的任何一个(但不包括正向和反向引物)或其活性变体及其片段或其互补序列的任何一种或多种靶基因,其中一个或多个 目标害虫中更多的序列控制害虫(即具有杀虫活性)。 还提供了包含本发明的沉默元件或其活性变体或片段的植物,植物部分,细菌和其它宿主细胞。
    • 9. 发明申请
    • COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS
    • 控制昆虫害虫的组合物和方法
    • WO2014153254A9
    • 2014-11-06
    • PCT/US2014029805
    • 2014-03-14
    • PIONEER HI BRED INTDU PONT
    • HU XUPRESNAIL JAMESRICHTMAN NINADIEHN SCOTTVAN ALLEN MICHELLEPROCYK LISA
    • C12N15/8218C12N15/8286Y02A40/162
    • Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
    • 提供了使用沉默元件的方法和组合物,所述沉默元件当被有害生物例如鞘翅目植物害虫或Diabrotica植物害虫摄入时降低害虫中靶序列的表达。 本发明提供了本文公开的任何SEQ ID NO:(但不包括正向和反向引物)或其活性变体和片段或其互补物中列出的各种靶多核苷酸,其中一个或多个 目标害虫中的更多序列控制害虫(即具有杀虫活性)。 还提供了包含本发明的沉默元件或其活性变体或片段的植物,植物部分,细菌和其他宿主细胞。