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2. 发明申请

WO2004090156A2 TEST SYSTEM 审中-公开
标题翻译：测试系统
公开(公告)号：WO2004090156A2
公开(公告)日：2004-10-21
申请号：PCT/GB2004/001578
申请日：2004-04-08
申请人： CROWN VISION SYSTEMS LIMITED , LEHARNE, Steven , NAGLER, Martin , NEAL, Gordon , EZZI, Mufaddal , COKER, Raymond , BELL, Edward , ROTHER, Joachim , PHILLIPS, Pamela , DAVIES, Lyn , ANDREOU, Michael , SOARED, Fernando
发明人： LEHARNE, Steven , NAGLER, Martin , NEAL, Gordon , EZZI, Mufaddal , COKER, Raymond , BELL, Edward , ROTHER, Joachim , PHILLIPS, Pamela , DAVIES, Lyn , ANDREOU, Michael , SOARED, Fernando
IPC分类号： C12Q1/00
CPC分类号： C12Q1/025
摘要： An assay is provided for assaying a sample of soil, sand, sediment or other particulate material for the presence of contaminants such as polycyclic aromatic hydrocarbons (PAHs), organic pesticides, petroleum hydrocarbons, or polychlorinated biphenyls (PCBs), and preferably comprises: extracting an assayable amount of the contaminant from the particulate material into a water-miscible solvent, such as an organic alcohol, capable of dissolving the contaminant, the solvent optionally containing a surfactant; mixing the resultant solvent solution of the contaminant with water and optionally a surfactant, for example to a dilution factor between about 10 and about 25, whereby a mixture is obtained containing water, solvent, surfactant and any extracted contaminant; and exposing the bioluminescent organism Vibrio fischeri to the mixture under conditions in which the inhibition, by the contaminant, of light emitted by the organism can be related to the presence of the contaminant in the mixture. The method is sufficiently quantitative that it can be determined whether the contaminant is present in the particulate material at a concentration above or below a certain - e.g. a legally specified - level. A test kit is provided, whereby the method can be performed in the field and the result showing whether the contaminant is present at a legally acceptable or unacceptable level can be displayed.
摘要翻译：提供了一种用于测定土壤，沙子，沉积物或其他颗粒材料样品中存在污染物如多环芳烃（PAHs），有机农药，石油烃或多氯联苯（PCBs）的测定法，优选包括：提取污染物从颗粒物质分解成能够溶解污染物的水混溶性溶剂如有机醇，任选地含有表面活性剂的溶剂; 将所得到的污染物溶剂与水和任选的表面活性剂混合，例如稀释因子为约10至约25，由此得到含有水，溶剂，表面活性剂和任何提取的污染物的混合物; 以及在生物体发射的光的抑制可能与混合物中污染物的存在有关的条件下，将生物发光生物体弧菌暴露于混合物中。该方法是足够定量的，可以确定污染物是否以高于或低于某一特定浓度的浓度存在于颗粒材料中。一个法定指定级别。提供了一种测试工具，由此可以在现场进行该方法，并且可以显示显示污染物是否以法律上可接受或不可接受的水平存在的结果。

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