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1. 发明申请

WO2013121224A1 ANALYSIS OF MEASUREMENTS OF A POLYMER 审中-公开
标题翻译：聚合物测量分析
公开(公告)号：WO2013121224A1
公开(公告)日：2013-08-22
申请号：PCT/GB2013/050381
申请日：2013-02-18
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： REID, Stuart William , CLARKE, James Anthony , WHITE, James , HARPER, Gavin
IPC分类号： C12Q1/68 , B82Y15/00 , G01N33/487 , G06F19/22
CPC分类号： G01N33/48792 , B82Y15/00 , C12Q1/6858 , C12Q1/6869 , G01N27/02 , G01N33/48721 , G01N33/6803 , G01N33/6875 , G06F19/22 , C12Q2537/165 , C12Q2565/631
摘要： A time-ordered series of measurements of a polymer made during translocation of the polymer through a nanopore are analysed. The measurements are dependent on the identity of k-mers in the nanopore, a k-mer being k polymer units of the polymer, where k is a positive integer. The method involves deriving, from the series of measurements, a feature vector of time-ordered features representing characteristics of the measurements; and determining similarity between the derived feature vector and at least one other feature vector.
摘要翻译：分析了在聚合物通过纳米孔的位移期间制备的聚合物的时间有序的一系列测量。测量取决于纳米孔中的k-mers的同一性，k-mer是聚合物的聚合物单元，其中k是正整数。该方法包括从一系列测量中导出表示测量特征的时间有序特征的特征向量; 以及确定所导出的特征向量与至少一个其他特征向量之间的相似度。

2. 发明申请

WO2016059427A1 ANALYSIS OF A POLYMER 审中-公开
标题翻译：聚合物分析
公开(公告)号：WO2016059427A1
公开(公告)日：2016-04-21
申请号：PCT/GB2015/053083
申请日：2015-10-16
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： REID, Stuart William , HARPER, Gavin , BROWN, Clive Gavin , TURNER, Daniel John , HERON, Andrew John , WRIGHT, Christopher James
IPC分类号： C12Q1/68 , G01N33/487 , G06F19/22
CPC分类号： C12Q1/6869 , G01N33/48721 , G06F19/22 , C12Q2565/631
摘要： Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
摘要翻译：聚合物的分析生化分析系统通过在聚合物穿过纳米孔的位移期间从包含纳米孔的传感器元件中测量聚合物来分析聚合物。当聚合物部分移位时，使用从参考序列得到的参考数据来分析一系列测量结果，以提供相似性的度量。响应于相似性的测量，可以选择性地操作传感器元件以喷射聚合物，从而使纳米孔可用于接收另外的聚合物。当生物化学分析系统包括传感器元件阵列并且以多路复用方式选择的传感器元件进行测量时，响应于相似性的测量，生物化学分析系统停止从当前选择的传感器元件进行测量并开始从新选择的传感器元件。

3. 发明申请

WO2013014451A1 HAIRPIN LOOP METHOD FOR DOUBLE STRAND POLYNUCLEOTIDE SEQUENCING USING TRANSMEMBRANE PORES 审中-公开
标题翻译：双链多核苷酸序列使用转化子簇的毛发环路方法
公开(公告)号：WO2013014451A1
公开(公告)日：2013-01-31
申请号：PCT/GB2012/051786
申请日：2012-07-25
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED , BROWN, Clive , CLARKE, James , HALL, Graham , HARPER, Gavin , HERON, Andrew , WHITE, James
发明人： BROWN, Clive , CLARKE, James , HALL, Graham , HARPER, Gavin , HERON, Andrew , WHITE, James
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , G01N27/44717 , G01N27/44791 , G01N33/48721 , C12Q2525/301 , C12Q2537/1376 , C12Q2565/631 , C12Q2535/119
摘要： The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
摘要翻译：本发明涉及测序双链靶多核苷酸的新方法。双链靶多核苷酸的两条链通过桥连部分连接。使用多核苷酸结合蛋白分离目标多核苷酸的两条链，并使用跨膜孔对靶多核苷酸进行测序。

4. 发明申请

WO2014096830A1 ANALYSIS OF A POLYNUCLEOTIDE VIA A NANOPORE SYSTEM 审中-公开
标题翻译：通过纳米系统分析多核苷酸
公开(公告)号：WO2014096830A1
公开(公告)日：2014-06-26
申请号：PCT/GB2013/053359
申请日：2013-12-19
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： REID, Stuart William , HARPER, Gavin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q2525/161 , C12Q2525/197 , C12Q2565/631
摘要： A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide. The predetermined sequence of the clock nucleotides comprises a restriction site for a restriction enzyme and at least one further nucleotide that extends the predetermined sequence.
摘要翻译：靶多核苷酸被扩增。关于靶多核苷酸中的每个核苷酸，靶多核苷酸以预定顺序包括时钟核苷酸和至少一个信号核苷酸。时钟核苷酸具有靶多核苷酸中每个核苷酸共有的预定序列。至少一个信号核苷酸是目标多核苷酸中相应核苷酸的特征。在扩增的多核苷酸在通过纳米孔的移位期间，进行取决于孔内的多核苷酸的电测量，以得到分析信号。识别从时钟核苷酸得到的时钟信号。相对于识别的时钟信号的位置，衍生自至少一个信号核苷酸的核苷酸信号被导出以分析靶多核苷酸。时钟核苷酸的预定序列包含限制酶的限制性位点和延伸预定序列的至少一个其它核苷酸。

5. 发明申请

WO2013041878A1 ANALYSIS OF A POLYMER COMPRISING POLYMER UNITS 审中-公开
标题翻译：包含聚合物单体的聚合物分析
公开(公告)号：WO2013041878A1
公开(公告)日：2013-03-28
申请号：PCT/GB2012/052343
申请日：2012-09-21
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： REID, Stuart William , HARPER, Gavin , BROWN, Clive Gavin , CLARKE, James Anthony , HERON, Andrew John
IPC分类号： C12Q1/68 , G01N33/487 , G06F19/22 , B82Y15/00
CPC分类号： G01N33/48721 , B82Y15/00 , C12Q1/6869 , G01N27/44791 , G01N33/483 , G06F17/18 , G06F19/22 , G06N7/005 , C12Q2537/165 , C12Q2565/631
摘要： A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.
摘要翻译：聚合物（3）中的聚合物单元序列，例如 DNA，从与聚合物相关的至少一系列测量估计，例如。作为通过纳米孔（1）的易位的函数的离子电流，其中每个测量的值取决于作为k个聚合物单元（4）的组的k-mer。提供概率模型，特别是隐马尔可夫模型（HMM），包括一组可能的k-mers：转换权重，表示从原始k-mers到目的地k-mers的转换机会; 和每个k-mer的发射权重，其表示观察该k-mer的给定值的测量值的机会。使用分析技术分析一系列测量结果，例如。维特比解码，其是指模型并且基于通过由聚合物单元的序列产生的一系列测量的模型预测的可能性估计聚合物中的聚合物单元的至少一个估计序列。在另一个实施方案中，在易位期间跨纳米孔施加不同的电压，以改善聚合物单元的分辨率。

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