专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2011067378A1 METHOD FOR AMPLIFICATION OF TARGET NUCLEIC ACID 审中-公开
标题翻译：放射性核酸的方法
公开(公告)号：WO2011067378A1
公开(公告)日：2011-06-09
申请号：PCT/EP2010/068837
申请日：2010-12-03
申请人： OLINK GENOMICS AB , ERICSSON, Olof , ISAKSSON, Magnus , JOHANSSON, Henrik , ROOS, Fredrik , FREDRIKSSON, Simon , NILSSON, Mats
发明人： ERICSSON, Olof , ISAKSSON, Magnus , JOHANSSON, Henrik , ROOS, Fredrik , FREDRIKSSON, Simon , NILSSON, Mats
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2565/519 , C12Q2531/125 , C12Q2563/131 , C12Q2525/161 , C12Q2521/501
摘要： The present invention provides a method for amplifying at least one target nucleic acid in a nucleic acid sample, comprising:(a) fragmenting the nucleic acid sample to create at least one target fragment comprising said target nucleic acid and comprising two probe-complementary portions, wherein at least one of said two probe-complementary portions is located at an end of the target fragment; (b) contacting said fragmented nucleic acid sample with at least one probe which is provided with an immobilisation moiety and optionally is immobilised on a solid phase by means of said moiety, and which comprises two target fragment-complementary portions which are complementary in sequence to the probe- complementary portions of the target fragment, wherein said portions of the probe may be adjacent, or separated by an intervening non-target fragment-complementary portion; (c) rendering the fragmented nucleic acid sample single- stranded, wherein the step may occur before, contemporaneously with or after step (b); (d) allowing the probe-complementary portions of the target fragment to hybridise with the target-fragment complementary portions of the probe; (e) if the probe provided in step (b) is not immobilised, immobilising the probe-target fragment hybrid on a solid phase by means of said immobilisation moiety; (f) separating non-immobilised nucleic acid fragments from the solid phase; (g) contacting the solid phase with a ligase to ligate, directly or indirectly, ligatable 5' and 3' ends of the target fragment whereby the target fragment is circularised, wherein where one of said probe-complementary portions of the target fragment is not located at an end of the target fragment, the ligatable end, other than that at which a probe-complementary portion is located, is created by the action of a flap endonuclease or an exonuclease; and (h) amplifying said circularised target fragment. Kits for use in the method of the invention are also provided.
摘要翻译：本发明提供了用于扩增核酸样品中的至少一种靶核酸的方法，其包括：（a）使核酸样品片段化以产生至少一个包含所述靶核酸的靶片段并且包含两个探针互补部分，其中所述两个探针互补部分中的至少一个位于所述靶片段的末端; （b）使所述片段化的核酸样品与至少一个具有固定部分的探针接触，并且任选地通过所述部分固定在固相上，并且其包含两个靶序列互补部分，其与序列互补所述靶片段的探针互补部分，其中所述探针的所述部分可以相邻，或由介入的非靶片段互补部分分开; （c）使所述片段化的核酸样品单链，其中所述步骤可以在步骤（b）之前或之后发生; （d）允许靶片段的探针互补部分与探针的靶 - 片段互补部分杂交; （e）如果步骤（b）中提供的探针未被固定，则通过所述固定部分将探针 - 靶片段杂交体固定在固相上; （f）从固相中分离未固定的核酸片段; （g）使固相与连接酶接触直接或间接连接目标片段的5'和3'末端，由此靶片段是环化的，其中靶片段的所述探针互补部分之一不是位于靶片段的末端，通过翼片内切核酸酶或外切核酸酶的作用产生除了探针互补部分所位于之外的可连接末端; 和（h）扩增所述环化的靶片段。还提供了用于本发明方法的试剂盒。

2. 发明申请

WO2011009941A1 PROBES FOR SPECIFIC ANALYSIS OF NUCLEIC ACIDS 审中-公开
标题翻译：具体分析核酸的探讨
公开(公告)号：WO2011009941A1
公开(公告)日：2011-01-27
申请号：PCT/EP2010/060715
申请日：2010-07-23
申请人： OLINK GENOMICS AB , ERICSSON, Olof , ISAKSSON, Magnus , JOHANSSON, Henrik , LANDEGREN, Ulf
发明人： ERICSSON, Olof , ISAKSSON, Magnus , JOHANSSON, Henrik , LANDEGREN, Ulf
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855
摘要： The present invention provides a method for detecting or enriching for a target deoxyribonucleic acid (DNA) present in a nucleic acid sample, said method comprising: (a) fragmenting a nucleic acid sample to generate nucleic acid fragments including a target fragment containing said target DNA; (b) rendering said fragments, including said target fragment, at least partially single-stranded, wherein the single- stranded portion includes an end portion and wherein the length of said single- stranded portion is sufficient to allow hybridisation of at least part of the single- stranded portion of said target fragment to the probe of step (c); (c) contacting the at least partially single-stranded fragments of step (b) with oligonucleotides A and B of a single target-specific nucleic acid probe, wherein: (i) oligonucleotide A is a single- stranded oligonucleotide comprising at one end a first target-specific part comprising at least 10 nucleotides complementary in sequence to at least part of said single- stranded portion of said target fragment, and comprising at the other end a second non-target-specific part which comprises a nucleotide sequence complementary to at least a portion, including one end, of oligonucleotide B of the probe, and (ii) oligonucleotide B is a single-stranded oligonucleotide which may contain or carry at least one element for detection and/or enrichment of said target fragment, and of which at least a portion, including one end, is complementary in sequence to the second non-target-specific part of oligonucleotide A, such that said target fragment becomes annealed to said probe through hybridisation to the first target-specific part of oligonucleotide A resulting in only one target-specific probe-binding event per target fragment; (d) ligating oligonucleotide B of said probe to the part of the single- stranded portion of said target fragment which is hybridised to oligonucleotide A of said probe to produce a probe-target fragment hybrid; and (e) detecting or enriching for said probe-target fragment hybrid. Kits for use in the method of the invention are also provided.
摘要翻译：本发明提供了用于检测或富集存在于核酸样品中的靶脱氧核糖核酸（DNA）的方法，所述方法包括：（a）将核酸样品分段以产生包含含有所述靶DNA的靶片段的核酸片段 ; （b）使包含所述靶片段的所述片段至少部分单链，其中所述单链部分包含端部，并且其中所述单链部分的长度足以允许杂交的至少一部分所述靶片段的单链部分与步骤（c）的探针结合; （c）将步骤（b）的至少部分单链片段与单个靶特异性核酸探针的寡核苷酸A和B接触，其中：（i）寡核苷酸A是单链寡核苷酸，其包含一端第一靶特异性部分包含与所述靶片段的所述单链部分的至少部分序列至少互补的10个核苷酸，并且在另一端包含第二非靶特异性部分，其包含与探针的寡核苷酸B的至少一部分（包括一端），和（ii）寡核苷酸B是可以含有或携带至少一种用于检测和/或富集所述靶片段的元件的单链寡核苷酸，其中包括一端的至少一部分在序列上与寡核苷酸A的第二非目标特异性部分互补，使得所述靶片段通过杂交退火至所述探针到寡核苷酸A的第一靶特异性部分，导致每个靶片段仅一个靶特异性探针结合事件; （d）将所述探针的寡核苷酸B连接到与所述探针的寡核苷酸A杂交的所述靶片段的单链部分的部分，以产生探针 - 靶片段杂交体; 和（e）检测或富集所述探针 - 靶片段杂交体。还提供了用于本发明方法的试剂盒。

3. 发明申请

WO2009091310A3 A VENTILATING ARRANGEMENT AND A HEAT EXCHANGING ASSEMBLY 审中-公开
公开(公告)号：WO2009091310A3
公开(公告)日：2009-07-23
申请号：PCT/SE2008/051277
申请日：2008-11-07
申请人： ERIKSSON, Per , ERIKSSON, Mats , ISAKSSON, Magnus
发明人： ERIKSSON, Per , ERIKSSON, Mats , ISAKSSON, Magnus
IPC分类号： F24F7/08 , F24F12/00
摘要： A ventilating arrangement for ventilating spaces (4) in a building has at least one heat exchanging assembly for heat exchange between exhaust air from the building and outdoor air supplied to the building. An outdoor air channel (14) conducts through an opening (12) in the external wall of the building outdoor air to the heat exchanging assembly. Exhaust air is conducted through exhaust air members (7) to the heat exchanging assembly. The flow of air to the spaces from the exterior of the building through supply air members (11) connected to the heat exchanging assembly only takes place through the negative air pressure generated in the spaces of the building through a flow of exhaust air leaving the spaces through said exhaust air members.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式