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1. 发明申请

WO2007062664A2 ENZYMATIC ENCODING METHODS FOR EFFICIENT SYNTHESIS OF LARGE LIBRARIES 审中-公开
标题翻译：用于大型图书馆有效合成的酶编码方法
公开(公告)号：WO2007062664A2
公开(公告)日：2007-06-07
申请号：PCT/DK2006000685
申请日：2006-12-01
申请人： NUEVOLUTION AS , FRANCH THOMAS , LUNDORF MIKKEL DYBRO , JAKOBSEN SOEREN NYBOE , OLSEN EVA KAMPMANN , ANDERSEN ANNE LEE , HOLTMANN ANETTE , HANSEN ANDERS HOLM , SOERENSEN ANDERS MALLING , GOLDBECH ANNE , DE LEON DAEN , KALDOR DITTE KIVSMOSE , SLOEK FRANK ABILGAARD , HUSEMOEN GITTE NYSTRUP , DOLBERG JOHANNES , JENSEN KIM BIRKEBAEK , PETERSEN LENE , NOERREGAARD-MADSEN MADS , GODSKESEN MICHAEL ANDERS , GLAD SANNE SCHROEDER , NEVE SOEREN , THISTED THOMAS , KRONBORG TINE TITILOLA AKINLEM , SAMS CHRISTIAN , FELDING JAKOB , FRESKGAARD PER-OLA , GOULIAEV ALEX HAAHR , PEDERSEN HENRIK
发明人： FRANCH THOMAS , LUNDORF MIKKEL DYBRO , JAKOBSEN SOEREN NYBOE , OLSEN EVA KAMPMANN , ANDERSEN ANNE LEE , HOLTMANN ANETTE , HANSEN ANDERS HOLM , SOERENSEN ANDERS MALLING , GOLDBECH ANNE , DE LEON DAEN , KALDOR DITTE KIVSMOSE , SLOEK FRANK ABILGAARD , HUSEMOEN GITTE NYSTRUP , DOLBERG JOHANNES , JENSEN KIM BIRKEBAEK , PETERSEN LENE , NOERREGAARD-MADSEN MADS , GODSKESEN MICHAEL ANDERS , GLAD SANNE SCHROEDER , NEVE SOEREN , THISTED THOMAS , KRONBORG TINE TITILOLA AKINLEM , SAMS CHRISTIAN , FELDING JAKOB , FRESKGAARD PER-OLA , GOULIAEV ALEX HAAHR , PEDERSEN HENRIK
IPC分类号： C12N15/10 , C12Q1/68 , C40B40/06 , C40B50/06
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
摘要翻译：公开了一种获得包含与单链标识符寡核苷酸连接的分子的双功能复合物的方法，其中包含化学反应位点的新生双功能复合物和用于酶标添加的引发位点是a）在化学反应位点与一种或多种反应物，和b）在起始位点用一种或多种标记反应物的标签进行酶促反应。

2. 发明申请

WO2004024929A3 PROXIMITY-AIDED SYNTHESIS OF TEMPLATED MOLECULES 审中-公开
标题翻译：近模助分解模板分子的合成
公开(公告)号：WO2004024929A3
公开(公告)日：2004-05-06
申请号：PCT/DK0300590
申请日：2003-09-12
申请人： NUEVOLUTION AS , FRANCH THOMAS , THISTED THOMAS
发明人： FRANCH THOMAS , THISTED THOMAS
IPC分类号： C12N15/10 , C07B61/00 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1068
摘要： Disclosed is a method for synthesising a bifunctional complex. The complex comprises a template as well as a molecule, the synthesis of which being directed by the template. The synthesis of he complex requires initially a) a template comprising two or more codons in sequence, a first pair of a molecular affinity pair, and a reactive group; and b) two or more building blocks, each building block comprises i) an anti-codon capable of recognising a codon of the template, ii) a functional entity comprising at least one reactive group, and iii) a linker connecting the anti-codon and the functional entity, wherein building blocks having anti-codons intended to interact with codons of the template distal to the reactive group comprise as a section of the linker a second part of the molecular affinity pair. The synthesis proceeds by c) contacting the template with a building block under conditions which allow specific hybridisation of the anti-codon of the building block to the codon of the template, and under conditions ensuring assembling of the parts of the molecule pair, if present; d) obtaining a connection between the functional entity of the building block and the template by a reaction involving the template reactive group and the functional entity reactive group, e) cleaving a linkage to obtaining a nascent templated molecule, f) separating the parts of the molecular affinity pair, and g) repeating, for a building block having an anti-codon capable of hybridising to a new codon, steps c) to f) one or more times. The complexes obtainable according the invention may be used in the generation of a library which may be enriched with regard to preferred complexes using molecular evolution techniques.
摘要翻译：公开了用于合成双功能复合物的方法。该复合物包含模板以及分子，其合成由模板引导。 he复合物的合成首先需要a）包含两个或更多个序列密码子的模板，第一对分子亲和对和反应基团; 和b）两个或更多个构件，每个构件包含i）能够识别模板密码子的反密码子，ii）包含至少一个反应基团的功能实体，以及iii）连接反密码子以及功能实体，其中具有意欲与反应性基团远端的模板的密码子相互作用的反密码子的构建块包含分子亲和对的第二部分作为接头的一部分。通过c）进行合成，c）使模板与构建模块在允许结构单元的反密码子与模板的密码子特异性杂交的条件下和在确保分子对的部分（如果存在的话）的组装 ; d）通过涉及模板反应性基团和功能性实体反应性基团的反应，获得构件的功能实体与模板之间的连接，e）裂解键以获得新生模板化分子，f）分离分子亲和对，和g）对于具有能够与新密码子杂交的反密码子的结构单元，重复步骤c）至f）一次或多次。根据本发明可获得的复合物可用于产生可利用分子进化技术就优选复合物而言富集的文库。

3. 发明申请

WO2004009814A8 GENE SHUFFLING BY TEMPLATE SWITCHING 审中-公开
标题翻译：基因切换通过模板切换
公开(公告)号：WO2004009814A8
公开(公告)日：2005-03-10
申请号：PCT/DK0300490
申请日：2003-07-11
申请人： NUEVOLUTION AS , PEDERSEN HENRIK , THISTED THOMAS , MOELLER HANS THORLEIF
发明人： PEDERSEN HENRIK , THISTED THOMAS , MOELLER HANS THORLEIF
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12P19/34 , C12N15/1027 , C12N15/1096 , C12Q1/6811
摘要： The present invention provides a new approach to creating novel polynucleotide sequences by point mutation and recombination in vitro of a set of parental sequences. The new polynucleotide sequences can be useful in themselves or they can be used for the templated synthesis of polymers, e.g. polypeptides composed of alpha-amino acids as occurring naturally in protein synthesis or polymers comprised of beta-amino acids or other building blocks as described (Templated molecules).
摘要翻译：本发明提供了一种通过点突变和体外重组一组亲本序列来产生新的多核苷酸序列的新方法。新的多核苷酸序列本身可以有用，或者它们可以用于聚合物的模板化合成，例如，由蛋白质合成中天然存在的α-氨基酸组成的多肽或由β-氨基酸或所述的其它结构单元（模板化分子）组成的聚合物。

4. 发明申请

WO2004074429A3 METHOD FOR PRODUCING SECOND-GENERATION LIBRARY 审中-公开
标题翻译：生成第二代库的方法
公开(公告)号：WO2004074429A3
公开(公告)日：2004-09-30
申请号：PCT/DK2004000117
申请日：2004-02-23
申请人： NUEVOLUTION AS , FRESKGAARD PER-OLA , GOULIAEV ALEX HAAHR , THISTED THOMAS , OLSEN EVA KAMPMANN
发明人： FRESKGAARD PER-OLA , GOULIAEV ALEX HAAHR , THISTED THOMAS , OLSEN EVA KAMPMANN
IPC分类号： C12N15/10 , C12Q1/68 , C07B61/00 , C12P1/00
CPC分类号： C40B40/08 , B01J19/0046 , B01J2219/00592 , C12N15/1068 , C12Q1/6811 , C40B50/04 , C40B50/06 , C40B50/10 , C12Q2565/501 , C12Q2531/113
摘要： The present invention relates to a method for generating a second-generation library. In a first step, a library of encoded molecules associated with an identifier nucleic acid comprising codons identifying chemical entities that have participated in the formation of the encoded molecule is provided. In a second step, the library is partitioned and encoded molecules having a certain property are selected. Codons of identifiers of selected encoded molecules are subsequently identified, and a second-generation library is prepared using at least some of the chemical entities coded for by the identified codons. The new focussed library may be used for another partition step to select encoded molecules with a certain property.
摘要翻译：本发明涉及用于生成第二代库的方法。在第一步中，提供了与识别核酸相关的编码分子文库，其包含识别已参与编码分子形成的化学实体的密码子。第二步，将文库分区并选择具有某种特性的编码分子。随后鉴定所选编码分子的标识符密码子，并使用至少一些由所鉴定的密码子编码的化学实体制备第二代文库。新聚焦库可用于另一个分区步骤来选择具有某种属性的编码分子。

5. 发明申请

WO02072876A3 SCREENING METHOD FOR THE SELECTION OF PEPTIDES OR PROTEINS COMPRISING DISCRETE DIMENSION ASSAYS, GENE MUTATION AND APPLICATION ASSAYS 审中-公开
标题翻译：选择包含离散尺寸测定的蛋白质或蛋白质的筛选方法，基因突变和应用测定
公开(公告)号：WO02072876A3
公开(公告)日：2002-12-12
申请号：PCT/DK0200160
申请日：2002-03-12
申请人： NOVOZYMES AS , KJAERULF SOEREN , LAMSA MICHAEL , THISTED THOMAS , HANSEN PETER KAMP , PEDERSEN HENRIK , FRISNER HENRIK
发明人： KJAERULF SOEREN , LAMSA MICHAEL , THISTED THOMAS , HANSEN PETER KAMP , PEDERSEN HENRIK , FRISNER HENRIK
IPC分类号： C12N15/10 , C12N15/09
CPC分类号： C12N15/1058
摘要： The present invention relates to a screening method for identifying actives (peptides or proteins) with improved performance comprising: (1) screening a library of actives encoded by nucleotide sequences in at least two different discrete dimension assays employing selected parameters and selecting the actives exhibiting improved properties in the discrete dimension assays, (2) collecting nucleotide sequences encoding the selected actives, (3) mutating collected nucleotide sequences, (4) screening actives encoded by the mutated nucleotide sequences in an application assay and selecting actives, which exhibit improved performance.
摘要翻译：本发明涉及用于鉴定具有改进性能的活性物质（肽或蛋白质）的筛选方法，其包括：（1）使用选择的参数在至少两个不同离散维度测定中筛选由核苷酸序列编码的活性物质文库，并选择显示改进的活性物质离子维度测定中的性质，（2）收集编码所选活性物质的核苷酸序列，（3）突变收集的核苷酸序列，（4）在应用测定中筛选由突变的核苷酸序列编码的活性物，并选择表现出改善的性能的活性物质。

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