专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

WO2012068627A1 PEPTIDES FOR THE SPECIFIC BINDING OF RNA TARGETS 审中-公开
标题翻译： RNA靶特异性结合的肽
公开(公告)号：WO2012068627A1
公开(公告)日：2012-05-31
申请号：PCT/AU2011/001519
申请日：2011-11-24
申请人： THE UNIVERSITY OF WESTERN AUSTRALIA , FILIPOVSKA, Aleksandra , RACKHAM, Oliver
发明人： FILIPOVSKA, Aleksandra , RACKHAM, Oliver
IPC分类号： C07K14/47 , C12N15/12 , A61K31/713 , C07K19/00 , C12N15/62 , A61K38/17
CPC分类号： C07K14/43581 , A61K38/00 , C07K14/43545 , C07K14/47 , C07K2319/02 , C07K2319/09 , C07K2319/20 , C07K2319/60 , C07K2319/85 , C12N15/64
摘要： A recombinant polypeptide is described which comprises at least one PUF RNA-binding domain capable of specifically binding to a cytosine RNA base. The PUF RNA-binding domain of the polypeptide comprises at least one RNA base-binding motif of the general formula X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 X 11 wherein X 1 is selected from the group including glutamine (Q), valine (V), methionine (M), proline (P), glutamic acid (E), and lysine (K); X 2 is selected from the group including histidine (H), phenylalanine (F), tyrosine (Y), and asparagine (N); X 3 is selected from the group including glycine (G) and alanine (A); X 4 is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X 5 is selected from the group including arginine (R), tyrosine (Y), histidine (H), and asparagine (N); X 6 is selected from the group including phenylalanine (F), leucine (L), and valine (V); X 7 is selected from the group including isoleucine (I), leucine (L), and valine (V); X 8 is arginine (R); X 9 is selected from the group including leucine (L), lysine (K), arginine (R), glutamine (Q), and histidine (H); X 10 is selected from the group including lysine (K), phenylalanine (F), alanine (A), cysteine (C), isoleucine (I), valine 1 (V), leucine (L), and methionine (M); and X 11 is selected from the group including leucine (L), phenylalanine (F), isoleucine (I), and valine (V); and wherein the RNA base- binding motif is operably capable of specifically binding to a cytosine RNA base.
摘要翻译：描述了包含能够特异性结合胞嘧啶RNA碱基的至少一个PUF RNA结合结构域的重组多肽。多肽的PUF RNA结合结构域包含至少一种通式为X1X2X3X4X5X6X7X8X9X10X11的RNA碱基结合基序，其中X1选自谷氨酰胺（Q），缬氨酸（V），甲硫氨酸（M），脯氨酸（P），谷氨酸（E）和赖氨酸（K）; X2选自组氨酸（H），苯丙氨酸（F），酪氨酸（Y）和天冬酰胺（N）; X3选自甘氨酸（G）和丙氨酸（A）; X4选自甘氨酸（G），丙氨酸（A），丝氨酸（S），苏氨酸（T）和半胱氨酸（C）; X5选自精氨酸（R），酪氨酸（Y），组氨酸（H）和天冬酰胺（N）; X6选自苯丙氨酸（F），亮氨酸（L）和缬氨酸（V）; X7选自异亮氨酸（I），亮氨酸（L）和缬氨酸（V）; X8是精氨酸（R）; X9选自亮氨酸（L），赖氨酸（K），精氨酸（R），谷氨酰胺（Q）和组氨酸（H）; X10选自赖氨酸（K），苯丙氨酸（F），丙氨酸（A），半胱氨酸（C），异亮氨酸（I），缬氨酸1（V），亮氨酸（L）和甲硫氨酸（M） X11选自亮氨酸（L），苯丙氨酸（F），异亮氨酸（I）和缬氨酸（V）; 并且其中所述RNA碱基结合基序可操作地能够特异性结合胞嘧啶RNA碱基。

IPRDB

热门服务

关于我们

友情链接

联系方式