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1. 发明申请

WO2020181354A1 ENDOGENOUS PLANT EXPRESSION ENHANCER 审中-公开
公开(公告)号：WO2020181354A1
公开(公告)日：2020-09-17
申请号：PCT/CA2019/050319
申请日：2019-03-14
申请人： MITSUBISHI TANABE PHARMA CORPORATION , MEDICAGO INC. , NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
发明人： LAVOIE, Pierre-Olivier , D'AOUST, Marc-Andre , KATO, Ko , YAMASAKI, Shotaro
IPC分类号： C12N15/113 , A01H5/00 , C07K14/08 , C07K14/11 , C12N15/13 , C12N15/40 , C12N15/44 , C12N15/82 , C12N5/10 , C12P21/02
摘要： An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

2. 发明申请

WO2011035422A1 METHOD OF PREPARING PLANT-DERIVED VLPS 审中-公开
标题翻译：制备植物衍生VLPS的方法
公开(公告)号：WO2011035422A1
公开(公告)日：2011-03-31
申请号：PCT/CA2010/001488
申请日：2010-09-21
申请人： MEDICAGO INC. , VEZINA, Louis-Philippe , COUTURE, Manon , PAQUET, Dany , DARGIS, Michele , D'AOUST, Marc-Andre
发明人： VEZINA, Louis-Philippe , COUTURE, Manon , PAQUET, Dany , DARGIS, Michele , D'AOUST, Marc-Andre
IPC分类号： C12N7/02 , A61K39/12 , A61K39/145 , A61P31/16 , A61P37/04 , C12N7/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/5258 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant- derived VLPs are recovered from the filtered fraction.
摘要翻译：提供了植物来源的病毒样颗粒（VLPs）的制备方法。该方法可以包括获得植物或包含原生质体定位的VLP的植物物质，从植物或植物物质产生原生质体/原生质体级分和原生质体级分，以及回收载脂蛋白级分。载脂蛋白级分包含植物来源的VLP。或者，VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得，以产生消化部分。将消化的馏分过滤以产生过滤的馏分，并从过滤的馏分中回收来自植物的VLP。

3. 发明申请

WO2008151440A1 MODIFYING GLYCOPROTEIN PRODUCTION IN PLANTS 审中-公开
标题翻译：修改植物中的糖蛋白生产
公开(公告)号：WO2008151440A1
公开(公告)日：2008-12-18
申请号：PCT/CA2008/001139
申请日：2008-06-13
申请人： MEDICAGO, INC. , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
摘要： A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNTl-GaIT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNTl) fused to a catalytic domain of beta-l,4galactosyltransferase (GaIT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
摘要翻译：提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化谱的目的蛋白质的方法。该方法包括在植物内共表达编码编码杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述第一核苷酸序列包含融合到β-1,4galactosyltransferase的催化结构域的N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域 GaIT），与在植物中有活性的第一调节区可操作地连接的第一核苷酸序列和用于编码目标蛋白的第二核苷酸序列，第二核苷酸序列与在植物中有活性的第二调节区可操作地连接。共表达第一和第二核苷酸序列以合成目标蛋白，其包含在植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖。

4. 发明申请

WO2022047575A8 MODIFIED CORONAVIRUS STRUCTURAL PROTEIN 审中-公开
公开(公告)号：WO2022047575A8
公开(公告)日：2022-03-10
申请号：PCT/CA2021/051201
申请日：2021-08-31
申请人： MEDICAGO INC.
发明人： LAVOIE, Pierre-Olivier , D'AOUST, Marc-Andre
IPC分类号： C07K19/00 , A61K39/215 , A61K39/295 , A61P31/14 , A61P37/04 , C07K14/11 , C07K14/165 , C07K16/10 , C12N15/44 , C12N15/50 , C12N15/62 , C12N15/67 , C12N15/86 , C12N5/10 , C12N7/01 , C12P21/02 , C12N7/00
摘要： Modified coronaviras spike (S)-protein, virus-like particle (VLPs) comprising the modified S protein and nucleic acids encoding modified S protein are provided. Methods for modified S-protein and VLP production in a host or host cell are also described. The modified S-protein may comprise a transmembrane domain (TM) or portion of a TM, and a cytosolic tail (CT) or of a CT, wherein the CT or portion of the CT is derived from an influenza hemagglutinin (HA) protein and wherein the TM or portion of the TM is heterologous to the CT or portion of the CT. In addition a method for inducing immunity to a coronaviras infection in a subject, comprising administering a composition comprising the modified coronaviras (S)-protein or VLP to the subject is described.

5. 发明申请

WO2015103704A1 CPMV ENHANCER ELEMENTS 审中-公开
标题翻译： CPMV增强元件
公开(公告)号：WO2015103704A1
公开(公告)日：2015-07-16
申请号：PCT/CA2015/050009
申请日：2015-01-08
申请人： MEDICAGO INC.
发明人： LAVOIE, Pierre-Olivier , D'AOUST, Marc-Andre
IPC分类号： C12N15/113 , A01H5/00 , C07H21/04 , C12N15/13 , C12N15/33 , C12N15/44 , C12N15/82 , C12N15/41
CPC分类号： C12N15/8216 , C12N15/8251
摘要： An expression enhancer comprising a CPMV 5'UTR nucleotide sequence consisting of X nucleotides (CMPVX), where X=160, 155, 150, or 114 of SEQ ID NO:1, or consisting of a nucleotide sequence comprising from about 80% to 100% sequence similarity with CMPVX, where X=160, 155, 150, or 114 of SEQ ID NO:1SEQ ID NO:1 is provided. The expression enhancer may further comprise a stuffer sequence fused to the 3' end of the 5'UTR nucleotide sequence (CMPVX+, where X=160, 155, 150, or 114 of SEQ ID NO:1). The stuffer sequence may comprise one or more plant kozak sequences. Plants comprising the expression enhancer and methods using the expression enhancer are also described.
摘要翻译：包含由X核苷酸（CMPVX）组成的CPMV 5'UTR核苷酸序列的表达增强子，其中X = SEQ ID NO：1的X = 160,155,150或114，或由包含约80％至100 提供了与SEQ ID NO：1SEQ ID NO：1的X = 160,155,150或114的CMPVX的％序列相似性。表达增强子还可以包含与5'UTR核苷酸序列（CMPVX +，其中X = SEQ ID NO：1的X = 160,155,150或114）的3'末端融合的填充序列。填充序列可以包含一个或多个植物kozak序列。还描述了包含表达增强子的植物和使用表达增强子的方法。

6. 发明申请

WO2010003225A1 INFLUENZA VIRUS-LIKE PARTICLES (VLPS) COMPRISING HEMAGGLUTININ 审中-公开
标题翻译：包含血凝素的流感病毒样颗粒（VLPS）
公开(公告)号：WO2010003225A1
公开(公告)日：2010-01-14
申请号：PCT/CA2009/000926
申请日：2009-07-02
申请人： MEDICAGO, INC. , D'AOUST, Marc-Andre , COUTURE, Manon , ORS, Frédéric , TREPANIER, Sonia , LAVOIE, Pierre-Olivier , DARGIS, Michèle , VEZINA, Louis-Philippe , LANDRY, Nathalie
发明人： D'AOUST, Marc-Andre , COUTURE, Manon , ORS, Frédéric , TREPANIER, Sonia , LAVOIE, Pierre-Olivier , DARGIS, Michèle , VEZINA, Louis-Philippe , LANDRY, Nathalie
IPC分类号： C12N15/82 , A61K39/145 , A61P31/16 , C12N15/44 , C12N7/00 , C12N7/01 , C07K14/11
CPC分类号： C12N15/8257 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/58 , C07K14/005 , C07K2319/02 , C12N7/00 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2830/65
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了用于在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。该方法涉及在植物中表达A型/ California / 04/09型流感HA并通过尺寸排阻色谱法进行纯化。本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。本发明还涉及编码A / California / 04/09型流感HA的核酸以及载体。 VLP可用于配制流感疫苗，或可用于丰富现有疫苗。

7. 发明申请

WO2018170603A1 NOROVIRUS FUSION PROTEINS AND VLPS COMPRISING NOROVIRUS FUSION PROTEINS 审中-公开
公开(公告)号：WO2018170603A1
公开(公告)日：2018-09-27
申请号：PCT/CA2018/050352
申请日：2018-03-23
申请人： MEDICAGO INC.
发明人： LAVOIE, Pierre-Olivier , D'AOUST, Marc-Andre
IPC分类号： C12N15/82 , A01H5/00 , A61K39/12 , A61P31/14 , A61P37/04 , C07K14/08 , C07K16/20 , C12N15/40 , C12N15/62 , C12N15/63 , C12N5/10 , C12N7/01 , C12N7/04 , C12P21/02
摘要： Nucleic acids encoding norovirus VP1 fusion proteins and VLPs comprising the norovirus VP1 fusion proteins are provided. Methods for norovirus VP1 fusion protein and norovirus VLP production in plants are also described. The VP1 fusion protein comprises, a first sequence encoding an S domain derived from a first norovirus strain, and a second sequence encoding a P domain derived from a second norovirus strain.

8. 发明申请

WO2018058256A1 MODIFYING N-GLYCOSYLATION OF PLANT PROTEINS USING GDP-4-DEHYDRO-6-DEOXY-D-MANNOSE REDUCTASE (RMD) 审中-公开
标题翻译：用GDP-4-脱氢-6-脱氧-D-甘露糖还原酶（RMD）修饰植物蛋白的N-糖基化
公开(公告)号：WO2018058256A1
公开(公告)日：2018-04-05
申请号：PCT/CA2017/051165
申请日：2017-09-29
申请人： MEDICAGO INC. , GENOPOLE
发明人： D'AOUST, Marc-Andre , LAVOIE, Pierre-Olivier , FALCON DE LONGEVIALLE, Andeol
IPC分类号： C12N5/10 , A01H5/00 , C12N15/53 , C12N15/82 , C12N9/04 , C12P21/00
摘要： A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a GDP-4-dehydro-6-deoxy-D-mannose reductase (RMD) the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
摘要翻译：提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化分布的目的蛋白质的方法。所述方法包括在植物内共表达编码编码GDP-4-脱氢-6-脱氧-D-甘露糖还原酶（RMD）的第一核苷酸序列的核苷酸序列，所述第一核苷酸序列与第一调控区有效连接，所述第一调控区是活性的以及编码感兴趣蛋白质的第二核苷酸序列，第二核苷酸序列与在植物中有活性的第二调控区有效连接。共表达第一和第二核苷酸序列以合成包含植物内植物，植物部分或植物细胞内具有修饰的N-糖基化分布的聚糖的目的蛋白质。

9. 发明申请

WO2017000074A1 JASMONIC ACID PATHWAY ACTIVATOR 审中-公开
公开(公告)号：WO2017000074A1
公开(公告)日：2017-01-05
申请号：PCT/CA2016/050772
申请日：2016-06-30
申请人： MEDICAGO INC. , UNIVERSITE LAVAL
发明人： D'AOUST, Marc-Andre , ROBERT, Stephanie , GOULET, Marie-Claire , MICHAUD, Dominique , SAINSBURY, Frank
IPC分类号： A01H5/00 , C12N15/09 , C12N15/82
CPC分类号： C12N15/8216 , A01H3/04 , C12N15/8257
摘要： A method of increasing expression of an heterologous protein of interest in a plant or portion of the plant is provided. The method comprises treating the plant or portion of the plant with ajasmonate-pathway activator, and introducing a nucleotide sequence operably linked to a regulatory region derived from a DNA plant virus and encoding the heterologous protein of interest into the plant or portion of the plant. Alternatively, the plant or plant portion may comprise the nucleic acid and encoding the heterologous protein of interest, and the plant or portion of the plant is treated with the jasmonate pathway activator. The treated plant is incubated under conditions to permit expression of the nucleotide sequence encoding the heterologous protein of interest.
摘要翻译：提供了增加植物或植物部分中感兴趣的异源蛋白质表达的方法。该方法包括用茉莉酮酸通路活化剂处理植物或部分植物，并引入与源自DNA植物病毒的调节区域可操作地连接并将感兴趣的异源蛋白质编码到植物的植物或部分中的核苷酸序列。或者，植物或植物部分可以包含核酸并编码感兴趣的异源蛋白质，并且用茉莉酮酸途径活化剂处理植物或植物部分。将处理的植物在允许表达编码感兴趣的异源蛋白质的核苷酸序列的条件下孵育。

10. 发明申请

WO2016115630A1 ROTAVIRUS-LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
标题翻译：罗非鱼样植物生产粒子
公开(公告)号：WO2016115630A1
公开(公告)日：2016-07-28
申请号：PCT/CA2016/050043
申请日：2016-01-21
申请人： MEDICAGO INC. , MITSUBISHI TANABE PHARMA CORPORATION
发明人： LAVOIE, Pierre-Olivier , D'AOUST, Marc-Andre
IPC分类号： C12N7/04 , A01H5/00 , C12N15/46 , C12N15/82 , C12N15/86 , C12N7/01 , C12N7/02
CPC分类号： C12N7/04 , A61K39/12 , A61K2039/5258 , C12N7/02 , C12N15/8257 , C12N15/86 , C12N2720/12322 , C12N2720/12323 , C12N2720/12334 , C12N2720/12351
摘要： A method of producing a rotavirus-like particle (RLP) in a plant is provided. The method comprises expressing within a host or host cell for example a plant, portion of a plant or plant cell one or more nucleic acid comprising one or more regulatory region operatively linked to a first, second and third nucleotide sequence, the regulatory region active in the host or host cell. The first nucleotide sequence encoding a first rotavirus protein, the second nucleotide sequence encoding a second rotavirus protein and the third nucleotide sequence encoding a third rotavirus protein. The first, second and third encode rotavirus protein NSP4 and VP2 or VP6 and VP4 or VP7. The host or host cell is incubated under conditions that permit the expression of the nucleic acids, so that NSP4 and either VP2 of VP6 and VP4 or VP7 are expressed, thereby producing the RLP. Hosts comprising the RLP, compositions comprising the RLP and method for using the composition are also provided.
摘要翻译：提供了一种在植物中产生轮状病毒样颗粒（RLP）的方法。该方法包括在宿主或宿主细胞内表达例如植物，植物或植物细胞的一部分，一个或多个核酸，其包含与第一，第二和第三核苷酸序列可操作地连接的一个或多个调节区，宿主或宿主细胞。编码第一轮状病毒蛋白的第一核苷酸序列，编码第二轮状病毒蛋白的第二核苷酸序列和编码第三轮状病毒蛋白的第三核苷酸序列。第一，第二和第三编码轮状病毒蛋白NSP4和VP2或VP6和VP4或VP7。宿主或宿主细胞在允许核酸表达的条件下孵育，使得VP6和VP4或VP7的NSP4和VP2都被表达，从而产生RLP。还提供了包含RLP的宿主，包含RLP的组合物和使用该组合物的方法。

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