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1. 发明申请

WO2010028382A2 COLLECTING AND PROCESSING COMPLEX MACROMOLECULAR MIXTURES 审中-公开
标题翻译：收集和处理复杂的大分子混合物
公开(公告)号：WO2010028382A2
公开(公告)日：2010-03-11
申请号：PCT/US2009056252
申请日：2009-09-08
申请人： MAYO FOUNDATION , AHLQUIST DAVID A , HARRINGTON JONATHAN J , ZOU HONGZHI , QUINT PATRICK S , TAYLOR WILLIAM R
发明人： AHLQUIST DAVID A , HARRINGTON JONATHAN J , ZOU HONGZHI , QUINT PATRICK S , TAYLOR WILLIAM R
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： B01L3/00 , A61B10/0038 , A61B10/0096 , B01L3/5029 , C12Q1/6806 , C12Q2565/507 , C12Q2563/143 , C12Q2527/125
摘要： This document provides methods and materials involved in collecting and processing complex macromolecular mixtures (e.g., stool samples). For example, stool collection devices, buffers for stabilizing nucleic acid and polypeptides present in stool, and kits for using sequence-specific capture probes (e.g., nucleic acid sequences designed to hybridize with particular target nucleic acids) to capture target nucleic acids directly from complex macromolecular mixtures (e.g., stool samples) without the need to perform prior steps to enrich, isolate, or purify the nucleic acid component are provided.
摘要翻译：本文件提供了收集和处理复杂大分子混合物（例如粪便样品）的方法和材料。例如，粪便收集装置，用于稳定存在于粪便中的核酸和多肽的缓冲液，以及使用序列特异性捕获探针（例如，设计成与特定靶核酸杂交的核酸序列）直接从复合物捕获靶核酸的试剂盒提供了大分子混合物（例如粪便样品），而不需要执行富集，分离或纯化核酸组分的先前步骤。

2. 发明申请

WO2009102788A2 DETECTING NEOPLASM 审中-公开
标题翻译：检测NEOPLASM
公开(公告)号：WO2009102788A2
公开(公告)日：2009-08-20
申请号：PCT/US2009033793
申请日：2009-02-11
申请人： MAYO FOUNDATION , TAYLOR WILLIAM R , HARRINGTON JONATHAN J , QUINT PATRICK S , ZOU HONGZHI , BERGEN III HAROLD R , SMITH DAVID I , AHLQUIST DAVID A
发明人： TAYLOR WILLIAM R , HARRINGTON JONATHAN J , QUINT PATRICK S , ZOU HONGZHI , BERGEN III HAROLD R , SMITH DAVID I , AHLQUIST DAVID A
IPC分类号： G01N33/574 , C12Q1/68 , G01N33/68
CPC分类号： C12Q1/6886 , C12Q1/37 , C12Q1/40 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158 , C12Y302/01001 , C12Y304/2107 , G01N33/57407 , G01N33/57419 , G01N33/57438 , G01N2333/928 , G01N2333/966 , G01N2560/00
摘要： This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.
摘要翻译：本文件涉及检测恶变前期和恶性肿瘤的方法和材料。例如，提供了用于确定来自哺乳动物的粪便样品是否含有肿瘤的核酸标记物或多肽标记物的方法和材料。

3. 发明申请

WO2012106525A3 DIGITAL SEQUENCE ANALYSIS OF DNA METHYLATION 审中-公开
标题翻译： DNA甲基化数字序列分析
公开(公告)号：WO2012106525A3
公开(公告)日：2014-02-20
申请号：PCT/US2012023646
申请日：2012-02-02
申请人： EXACT SCIENCES CORP , MAYO FOUNDATION , AHLQUIST DAVID A , TAYLOR WILLIAM R , ZOU HONGZHI , LIDGARD GRAHAM P
发明人： AHLQUIST DAVID A , TAYLOR WILLIAM R , ZOU HONGZHI , LIDGARD GRAHAM P
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q1/6858 , C12Q2600/154 , C12Q2521/331 , C12Q2523/125 , C12Q2563/159
摘要： The present invention relates to methods and compositions for determination of and uses of specific methylation patterns indicative of adenoma and carcinoma. In particular, the invention relates to analysis of defined CpG loci that are coordinately methylated in DNAs from cancer and adenoma samples, methods for identifying coordinately methylated loci, and methods of using analysis of coordinately methylated loci in one or more marker regions in the design of assays for adenoma and cancer.
摘要翻译：本发明涉及用于确定和使用指示腺瘤和癌症的特异性甲基化模式的方法和组合物。特别地，本发明涉及在来自癌症和腺瘤样品的DNA中配位甲基化的定义的CpG基因座的分析，用于鉴定配位甲基化位点的方法以及在设计中的一个或多个标记区域中使用配位甲基化位点分析的方法腺瘤和癌症检测。

4. 发明申请

WO2011119934A2 METHODS AND MATERIALS FOR DETECTING COLORECTAL NEOPLASM 审中-公开
标题翻译：检测色素沉积物的方法和材料
公开(公告)号：WO2011119934A2
公开(公告)日：2011-09-29
申请号：PCT/US2011029959
申请日：2011-03-25
申请人： ZOU HONGZHI , AHLQUIST DAVID , HARRINGTON JONATHAN J , MAYO FOUNDATION
发明人： ZOU HONGZHI , AHLQUIST DAVID , HARRINGTON JONATHAN J
IPC分类号： G01N33/68 , C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/154 , C12Q2600/156 , G01N33/57419 , G01N2333/805 , G01N2800/50 , G01N2800/7028
摘要： The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals having a colorectal neoplasm by detecting the presence of exfoliated epithelial markers (e.g., human DNA, tumor assoicated gene alterations, tumor associated proteins) and blood markers (e.g., homoglobin, serum proteins) in a stool sample obtained from the mammal.
摘要翻译：本发明提供与检测受试者粪便样品中或与受试者粪便样品相关的结肠直肠肿瘤特异性标志物相关的方法和材料。特别地，本发明提供了通过检测剥离的上皮标志物（例如，人类DNA，肿瘤分解的基因改变，肿瘤相关蛋白）和血液标志物（例如，血红蛋白，血清蛋白质）的存在来鉴定具有结肠直肠肿瘤的哺乳动物的方法和材料）在从哺乳动物获得的粪便样品中。

5. 发明申请

WO2011126768A3 METHODS AND MATERIALS FOR DETECTING COLORECTAL CANCER AND ADENOMA 审中-公开
标题翻译：检测大肠癌和腺瘤的方法和材料
公开(公告)号：WO2011126768A3
公开(公告)日：2012-02-23
申请号：PCT/US2011029982
申请日：2011-03-25
申请人： MAYO FOUNDATION , ZOU HONGZHI , AHLQUIST DAVID A
发明人： ZOU HONGZHI , AHLQUIST DAVID A
IPC分类号： C12Q1/68 , C12N15/12
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158
摘要： The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers (e.g., markers associated with colorectal cancer, markers associated with adenoma) in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals (e.g., humans) having a colorectal neoplasm by detecting the presence and level of indicators of colorectal neoplasia such as, for example, long DNA (e.g., quantified by Alu PCR) and the presence and level of tumor-associated gene alterations (e.g., mutations in KRAS, APC, melanoma antigen gene, p53, BRAF, BAT26, PIK3CA) or epigenetic alterations (e.g., DNA methylation) (e.g., CpG methylation) (e.g., CpG methylation in coding or regulatory regions of bmp-3, bmp-4, SFRP2, vimentin, septin9, ALX4, EYA4, TFPI2, NDRG4, FOXE1) in DNA from a stool sample obtained from the mammal.
摘要翻译：本发明提供与检测对象粪便样品中或与对象粪便样品相关的结肠直肠肿瘤特异性标志物（例如与结直肠癌相关的标志物，与腺瘤相关的标志物）有关的方法和材料。具体而言，本发明提供了用于通过检测结肠直肠瘤形成指标例如长DNA（例如，通过Alu PCR定量）的指标的存在和水平来鉴定具有结肠直肠肿瘤的哺乳动物（例如，人）的方法和材料，（例如，KRAS，APC，黑素瘤抗原基因，p53，BRAF，BAT26，PIK3CA中的突变）或表观遗传改变（例如DNA甲基化）（例如CpG甲基化）（例如CpG 甲基化在来自哺乳动物获得的粪便样品的DNA中的bmp-3，bmp-4，SFRP2，波形蛋白，septin9，ALX4，EYA4，TFPI2，NDRG4，FOXE1）的编码区或调节区中。

6. 发明申请

WO2011126768A9 METHODS AND MATERIALS FOR DETECTING COLORECTAL CANCER AND ADENOMA 审中-公开
标题翻译：检测彩色癌和腺癌的方法和材料
公开(公告)号：WO2011126768A9
公开(公告)日：2012-04-05
申请号：PCT/US2011029982
申请日：2011-03-25
申请人： MAYO FOUNDATION , ZOU HONGZHI , AHLQUIST DAVID A
发明人： ZOU HONGZHI , AHLQUIST DAVID A
IPC分类号： C12Q1/68 , C12N15/12
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158
摘要： The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers (e.g., markers associated with colorectal cancer, markers associated with adenoma) in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals (e.g., humans) having a colorectal neoplasm by detecting the presence and level of indicators of colorectal neoplasia such as, for example, long DNA (e.g., quantified by Alu PCR) and the presence and level of tumor-associated gene alterations (e.g., mutations in KRAS, APC, melanoma antigen gene, p53, BRAF, BAT26, PIK3CA) or epigenetic alterations (e.g., DNA methylation) (e.g., CpG methylation) (e.g., CpG methylation in coding or regulatory regions of bmp-3, bmp-4, SFRP2, vimentin, septin9, ALX4, EYA4, TFPI2, NDRG4, FOXE1) in DNA from a stool sample obtained from the mammal.
摘要翻译：本发明提供与检测与受试者粪便样品相关的结肠直肠肿瘤特异性标志物（例如，与结肠直肠癌相关的标志物，与腺瘤相关的标志物）相关的方法和材料。特别地，本发明提供了通过检测结肠直肠瘤形成指标（例如长DNA（例如，通过Alu PCR定量））的存在和水平来鉴定具有结肠直肠肿瘤的哺乳动物（例如，人）的方法和材料，以及肿瘤相关基因改变（例如，KRAS，APC，黑素瘤抗原基因，p53，BRAF，BAT26，PIK3CA中的突变）或表观遗传改变（例如，DNA甲基化）（例如，CpG甲基化）（例如，CpG 在来自哺乳动物的粪便样品的DNA中，bmp-3，bmp-4，SFRP2，波形蛋白，septin9，ALX4，EYA4，TFPI2，NDRG4，FOXE1的编码或调节区中的甲基化）。

7. 发明申请

WO2012067830A2 METHYLATION ASSAY 审中-公开
标题翻译：甲基化测定
公开(公告)号：WO2012067830A2
公开(公告)日：2012-05-24
申请号：PCT/US2011058997
申请日：2011-11-02
申请人： EXACT SCIENCES CORP , ZOU HONGZHI , LIDGARD GRAHAM P , DOMANICO MICHAEL J , ALLAWI HATIM
发明人： ZOU HONGZHI , LIDGARD GRAHAM P , DOMANICO MICHAEL J , ALLAWI HATIM
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q2600/154 , C12Q2521/301 , C12Q2523/125 , C12Q2561/109
摘要： A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3' terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.
摘要翻译：提供了一种检测甲基化基因组座位的方法。在某些实施方案中，所述方法包括：a）将含有基因组座位的非甲基化和甲基化拷贝的核酸样品用修饰胞嘧啶至尿嘧啶的试剂处理以产生经处理的核酸; b）使用第一引物和第二引物扩增来自经处理的核酸的产物，其中第一引物与含有甲基胞嘧啶的位点的位点杂交，并且扩增优先扩增基因组基因座的甲基化拷贝，以产生扩增的样品; 和c）使用采用具有对应于基因组基因座中的甲基化位点的3'末端G或C核苷酸的侵入性寡核苷酸的皮瓣测定来检测扩增样品中扩增的基因组座位的扩增的甲基化拷贝的存在。

8. 发明申请

WO2012155072A2 ISOLATION OF NUCLEIC ACIDS 审中-公开
标题翻译：分离核酸
公开(公告)号：WO2012155072A2
公开(公告)日：2012-11-15
申请号：PCT/US2012037581
申请日：2012-05-11
申请人： EXACT SCIENCES CORP , BRUINSMA JANELLE J , DOMANICO MICHAEL J , LIDGARD GRAHAM P , ZOU HONGZHI , WEISBURG WILLIAM G , SHENOI HEMANTH D , LIGHT II JAMES P
发明人： BRUINSMA JANELLE J , DOMANICO MICHAEL J , LIDGARD GRAHAM P , ZOU HONGZHI , WEISBURG WILLIAM G , SHENOI HEMANTH D , LIGHT II JAMES P
IPC分类号： C12Q1/68 , C12M1/12 , C12N15/10 , C12S3/20
CPC分类号： C12Q1/6806 , B01D33/15 , B01D33/155 , B01L3/00 , B01L3/5021 , B03C1/30 , B04B3/00 , C12N15/1006 , C12N15/1013 , C12N15/1017 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16 , Y10T436/143333
摘要： Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.
摘要翻译：本文提供了与分离核酸有关的技术。特别地，该技术涉及从有问题的样品如粪便中提取核酸的方法和试剂盒。

9. 发明申请

WO2013058868A3 MULTIPLEXED KRAS MUTATION DETECTION ASSAY 审中-公开
标题翻译：多重KRAS突变检测测定
公开(公告)号：WO2013058868A3
公开(公告)日：2014-05-15
申请号：PCT/US2012052377
申请日：2012-08-24
申请人： EXACT SCIENCES CORP , OLDHAM-HALTOM REBECCA , ALLAWI HATIM , ZOU HONGZHI , DOMANICO MICHAEL J , LIDGARD GRAHAM P
发明人： OLDHAM-HALTOM REBECCA , ALLAWI HATIM , ZOU HONGZHI , DOMANICO MICHAEL J , LIDGARD GRAHAM P
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q2525/161 , C12Q2561/109 , C12Q2565/1015
摘要： Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.
摘要翻译：本文提供的试剂混合物包含多重扩增试剂和皮瓣测定试剂，用于在单次反应中检测含有34A，34C，34T，35A，35C，35T或38A点突变中任何一种的KRAS基因的突变体拷贝。还提供了使用试剂混合物和试剂盒进行其的方法。

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