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1. 发明申请

WO2008051243A3 PATHOGEN-DETECTING CELL PRESERVATION SYSTEMS 审中-公开
标题翻译：病原体检测细胞保存系统
公开(公告)号：WO2008051243A3
公开(公告)日：2009-04-30
申请号：PCT/US2006045825
申请日：2006-11-30
申请人： MASSACHUSETTS INST TECHNOLOGY , PETROVICK MARTHA S , SCHWOEBEL ERIC , NARGI FRANCES , HARPER JAMES , RIDER TODD
发明人： PETROVICK MARTHA S , SCHWOEBEL ERIC , NARGI FRANCES , HARPER JAMES , RIDER TODD
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0226
摘要： Methods to produce cells that remain viable at ambient or non-refrigerated temperatures, or which can be stored in a dry state are described. In particular, cell preservation is for long term storage of cells, such as mammalian cells, at ambient or non-refrigerated temperatures, while retaining cell viability. Also provided are sensor cells that can detect target particles, biological agents or other materials and which remain viable at ambient or non-refrigerated temperatures, or which can be stored in a dry state.
摘要翻译：描述了生产在环境或非冷藏温度下保持活性或可以以干燥状态储存的细胞的方法。特别地，细胞保存用于在环境或非冷藏的温度下长期储存诸如哺乳动物细胞的细胞，同时保持细胞活力。还提供了可以检测目标颗粒，生物制剂或其它材料并且在环境或非冷藏温度下保持活性或可以以干燥状态储存的传感器细胞。

2. 发明申请

WO2008048300A2 PATHOGEN DETECTION BIOSENSOR 审中-公开
标题翻译：病原体检测生物传感器
公开(公告)号：WO2008048300A2
公开(公告)日：2008-04-24
申请号：PCT/US2006045691
申请日：2006-11-30
申请人： MASSACHUSETTS INST TECHNOLOGY , SCHWOEBEL ERIC , HARPER JAMES , PETROVICK MARTHA S , NARGI FRANCES , HOLLIS MARK , JOHNSON BERNADETTE , LACIRIGNOLA JOSEPH , MATHEWS RICHARD , HOGAN KRISTINE , VIAN TRINA , HEFF ALLAN , HENNESSY MARK , PALCHAUDHURI SONGEETA , RIDER TODD
发明人： SCHWOEBEL ERIC , HARPER JAMES , PETROVICK MARTHA S , NARGI FRANCES , HOLLIS MARK , JOHNSON BERNADETTE , LACIRIGNOLA JOSEPH , MATHEWS RICHARD , HOGAN KRISTINE , VIAN TRINA , HEFF ALLAN , HENNESSY MARK , PALCHAUDHURI SONGEETA , RIDER TODD
IPC分类号： G01N33/50
CPC分类号： G01N33/54373 , G01N21/6428 , G01N21/75
摘要： The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples.
摘要翻译：本文描述的本发明提供了用于检测靶颗粒如病原体，可溶性抗原，核酸，毒素，化学物质，植物病原体，血源性病原体，细菌，病毒等的方法。还描述了包含受体的发射体细胞，其中受体可以是抗体或Fc受体，以及用于检测样品中靶细胞的发射体分子，其中待检测的靶颗粒被一个或多个受体结合在发射器单元上。还提供了用于检测样品中的目标颗粒的光电传感器装置，包括在多个样品中。

3. 发明申请

WO03033740A2 APPARATUS AND METHOD FOR ISOLATING A NUCLEIC ACID 审中-公开
标题翻译：用于分离核酸的装置和方法
公开(公告)号：WO03033740A2
公开(公告)日：2003-04-24
申请号：PCT/US0221715
申请日：2002-07-10
申请人： MASSACHUSETTS INST TECHNOLOGY
发明人： PARAMESWARAN LALITHA , YOUNG ALBERT , BORTOLIN LAURA T , HOLLIS MARK A , HARPER JAMES , BOBROW JOHANNA
IPC分类号： B01L3/00 , B01L3/02 , C12N1/08 , C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： B01L3/0282 , B01L3/0241 , B01L3/5023 , B01L2400/0406 , B01L2400/0415 , B01L2400/049 , C12Q1/6806 , C12Q2565/137 , C12Q2549/125
摘要： A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The method can be conducted in an apparatus that includes a porous support having a component that deactivates a nucleic acid amplification inhibitor component of a sample contacting the porous support and a housing having an opening and defining an interior, said interior being in fluid communication with the porous support, whereby at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support and separates at least a portion of a nucleic acid component of a sample contacting the porous support from the support, thereby preparing the nucleic acid component for amplification.
摘要翻译：用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触，多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体，由此样品的核酸组分为引导通过多孔载体的至少一部分并与载体分离，由此制备用于扩增的核酸组分。该方法可以在包括多孔载体的装置中进行，所述多孔载体具有使与多孔载体接触的样品的核酸扩增抑制剂组分失活的组分和具有开口并限定内部的壳体，所述内部与多孔载体，其中通过开口引导的流体的至少一部分被引导通过多孔载体的至少一部分，并将与多孔载体接触的样品的核酸组分的至少一部分与载体分离，由此制备用于扩增的核酸组分。

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