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2. 发明申请

WO2008045505A3 COMPOSITIONS, METHODS AND KITS FOR ISOLATING NUCLEIC ACIDS FROM BODY FLUIDS USING ANION EXCHANGE MEDIA 审中-公开
标题翻译：使用阴离子交换介质从体液中分离核酸的组合物，方法和工具
公开(公告)号：WO2008045505A3
公开(公告)日：2008-09-18
申请号：PCT/US2007021723
申请日：2007-10-10
申请人： XENOMICS INC , FEAVER WILLIAM JOHN , MELKONYAN HOVSEP , UMANSKY SAMUIL , MEYER ERIK
发明人： FEAVER WILLIAM JOHN , MELKONYAN HOVSEP , UMANSKY SAMUIL , MEYER ERIK
IPC分类号： A23J1/00 , C07K1/00 , C07K14/00 , C07K16/00 , C07K17/00 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/101 , C12N15/1006 , C12N15/1017 , C12Q1/6806
摘要： This invention provides compositions and methods for rapid separation, isolation and purification of nucleopolymers, especially nucleic acids, from biological samples. using anionic exchange media. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The medium/nucleoprotein bound complex may be optionally stored or transported, but is subsequently treated with appropriate eluents to remove any undesirable proteins, including destabilizing enzymes, and inorganic salts and the like. The partially purified complex may also be stored or transported prior to further processing. The instant method is particularly advantageous as it provides the means for rapid isolation and purification of soluble nucleic acids obtained from samples of biological fluids of comparatively large volume, allowing ease of handling and storage, and stabilizing the samples against degradation prior to analysis. It also permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, up till now, had not been identified. The method is widely useful as a means to prepare samples formats that are convenient in diagnostic analytical methods, particularly those that rely on the detection, characterization and identification of nucleic acid sequences, from body fluids.
摘要翻译：本发明提供了用于从生物样品快速分离，分离和纯化核聚合物，特别是核酸的组合物和方法。使用阴离子交换介质。本发明的方法可以使用具有已知离子强度的选定溶液的市售强或弱阴离子交换材料进行吸附和洗脱。可以任选地储存或转运中等/核蛋白结合的复合物，然后用合适的洗脱液处理以去除任何不需要的蛋白质，包括去稳定化酶和无机盐等。部分纯化的复合物也可以在进一步加工之前储存或运输。本方法是特别有利的，因为其提供用于快速分离和纯化从相对较大体积的生物流体的样品获得的可溶性核酸的手段，允许易于处理和储存，以及在分析前稳定样品免于降解。它还允许纯化和鉴定来自体液的较短的核酸片段，迄今为止尚未鉴定出来。该方法广泛用作制备在诊断分析方法中方便的样品格式的手段，特别是依赖于来自体液的核酸序列的检测，表征和鉴定的样品。

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