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1. 发明申请

WO2012116386A1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 审中-公开
标题翻译：通过功能电极连接形成的生物传感器阵列
公开(公告)号：WO2012116386A1
公开(公告)日：2012-09-07
申请号：PCT/AT2012/000043
申请日：2012-02-27
申请人： LEXOGEN GMBH , REDA, Torsten , HAGLMÜLLER, Jakob , SCHITTER, Georg , SEITZ, Alexander
发明人： REDA, Torsten , HAGLMÜLLER, Jakob , SCHITTER, Georg , SEITZ, Alexander
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/6837 , B82Y15/00 , G01N27/3278 , G01N33/5438 , G01N33/553
摘要： We provide a sensor array device for the measurement of mixtures of organic compounds comprising an assembly of sensor half elements which have been functionalized through sensor compounds before the assembly. Each individual sensor of the array contains two sensor compounds which are bound at opposite sensor half elements. The molecular recognition is bi-functional. While the amount of sensor compounds increases linearly, the individual sensors increase with the second power.
摘要翻译：我们提供一种用于测量有机化合物混合物的传感器阵列装置，包括传感器半部件的组件，其在组装之前已通过传感器化合物进行功能化。阵列的每个单独的传感器包含两个传感器化合物，它们在相对的传感器半部分上结合。分子识别是双功能的。当传感器化合物的量线性增加时，各个传感器随着第二功率的增加而增加。

2. 发明申请

WO2012116385A1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 审中-公开
标题翻译：通过功能电极连接形成的生物传感器阵列
公开(公告)号：WO2012116385A1
公开(公告)日：2012-09-07
申请号：PCT/AT2012/000042
申请日：2012-02-27
申请人： LEXOGEN GMBH , REDA, Torsten , HAGLMÜLLER, Jakob , SCHITTER, Georg , SEITZ, Alexander
发明人： REDA, Torsten , HAGLMÜLLER, Jakob , SCHITTER, Georg , SEITZ, Alexander
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6837 , G01N27/3278
摘要： The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.
摘要翻译：本发明提供一种具有多个传感器接头的传感器阵列装置，其通过组装两个或多个不同功能化的表面而产生。在组装传感器阵列之前，当不同的表面彼此物理分离时，传感器化合物的预期传感器接合区域的功能化发生。通过这些方式，可以将传感器结形成为比常规沉积技术更小，如印刷和光刻将允许其他方式。因此，每个传感器接头都包含两种潜在的传感器化合物。传感器阵列识别和量化不同的生物分子。

3. 发明申请

WO2011095501A1 COMPLEXITIY REDUCTION METHOD 审中-公开
标题翻译：完全简化法
公开(公告)号：WO2011095501A1
公开(公告)日：2011-08-11
申请号：PCT/EP2011/051442
申请日：2011-02-02
申请人： LEXOGEN GMBH , SEITZ, Alexander , HAGLMÜLLER, Jakob , REDA, Torsten
发明人： SEITZ, Alexander , HAGLMÜLLER, Jakob , REDA, Torsten
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6869 , C12Q2523/301 , C12Q2537/143 , C12Q2525/191
摘要： The present invention relates to a method for the reduction of the complexity of nucleic acid pool(s), comprising - providing a sample with one or more nucleic acid molecules, - cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments, - amplifying one or more fragments of said nucleic acid molecules, wherein the one or more fragments constitute at least a fraction of all fragments of the nucleic acid molecules, and wherein the amplified or amplifying one or more fragments of said fraction are divided into different subpools, and wherein the fragments of each subpool comprise a common nucleic acid feature.
摘要翻译：本发明涉及降低核酸库的复杂性的方法，包括 - 提供具有一种或多种核酸分子的样品， - 通过将核酸分子切割随机和/或序列独立的切割步骤，从而获得核酸片段库， - 扩增所述核酸分子的一个或多个片段，其中所述一个或多个片段构成所述核酸分子的所有片段的至少一部分，以及其中所述部分的扩增或扩增的一个或多个片段被分成不同的亚类，并且其中每个亚类的片段包含共同的核酸特征。

4. 发明申请

WO2011070155A1 RNA ANALYTICS METHOD 审中-公开
标题翻译： RNA分析方法
公开(公告)号：WO2011070155A1
公开(公告)日：2011-06-16
申请号：PCT/EP2010/069382
申请日：2010-12-10
申请人： LEXOGEN GMBH , SEITZ, Alexander , PAUL, Lukas , VAN MIN, Max Jan
发明人： SEITZ, Alexander , PAUL, Lukas , VAN MIN, Max Jan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q2537/143 , C12Q2535/122 , C12Q2525/191 , C12Q2563/179 , C12Q2539/107
摘要： The present invention relates to a method of ordering nucleic acid molecule fragment sequences derived from a pool of potentially diverse RNA molecules comprising • optionally reverse transcribing the RNA molecules to provide a pool of cDNA molecules, • segregating nucleic acids from said template RNA or cDNA pool, selecting for potentially different templates with a distinctive nucleic acid feature shared by the segregated templates, thereby providing at least a first subpool of nucleic acids, • optionally once or more further segregating nucleic acids from said template RNA or cDNA, selectively segregating nucleic acids with a different distinctive nucleic acid feature, thereby providing one or more further subpool(s) of nucleic acids, • generating fragments of said segregated nucleic acid molecules by fragmenting or obtaining fragment copies of said segregated nucleic acid molecules, wherein the fragments of each subpool or combined subpools remain separable from fragments of other subpools or other combined subpools by physically separating the subpools or by attaching a label to the fragments of the subpools, with the label identifying a subpool, or determining a partial sequence of said segregated nucleic acid molecule and preferably aligning at least two sequences or partial sequences to a joined sequence.
摘要翻译：本发明涉及一种排序源自潜在不同RNA分子库的核酸分子片段序列的方法，其包括任选逆转录RNA分子以提供cDNA分子池，将核酸与所述模板RNA或cDNA库分离选择具有由分离的模板共享的独特的核酸特征的潜在不同的模板，从而提供核酸的至少第一子池，任选地一次或多次将所述模板RNA或cDNA分离出来的核酸，选择性地将核酸与不同的特征核酸特征，从而提供核酸的一个或多个另外的子区，通过片段化或获得所述分离的核酸分子的片段拷贝产生所述分离的核酸分子的片段，其中每个子池的片段或组合子项目与ot的片段保持分离她的子项目或其他组合子项目通过物理分离子项目或通过将标签附加到子项目的片段上，标签标识子项，或确定所述分离的核酸分子的部分序列，优选对齐至少两个序列或部分序列到连接序列。

5. 发明申请

WO2022069703A1 METHOD AND MEANS FOR GENERATING TRANSCRIBED NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2022069703A1
公开(公告)日：2022-04-07
申请号：PCT/EP2021/077088
申请日：2021-10-01
申请人： LEXOGEN GMBH
发明人： SURVILA, Mantas , MOLL, Pamela , SEITZ, Alexander
IPC分类号： C12Q1/6865 , C12Q2563/179
摘要： The present invention relates to a method of generating transcribed nucleic acids, comprising the steps of providing a nucleic acid template, hybridizing an oligonucleotide probe to the nucleic acid template, wherein said oligonucleotide probe comprises a complementary part that hybridizes to the nucleic acid template and a non-complementary part in (5') direction of the complementary part that does not hybridize to the nucleic acid template and comprises a sequence of a transcription promoter, hydrolysing a (3') part of the nucleic acid template that is in (3') direction to a part of the nucleic acid template that hybridizes to the oligonucleotide probe and wherein said (3') part is not hybridized to the oligonucleotide probe, or hydrolysing the nucleic acid template in the template-probe duplex to dissociate the (3') directed part of the template, extending the nucleic acid template with nucleic acids complementary to the non-complementary part of the oligonucleotide probe, thereby generating a duplex of the sequence of the transcription promoter in sequence with the nucleic acid template, transcribing the nucleic acid template with a transcriptase that binds the duplex of the sequence of the transcription promoter, thereby generating the transcribed nucleic acids; and kits and nucleic acids for performing such a method.

6. 发明申请

WO2014009413A2 5´ PROTECTION DEPENDENT AMPLIFICATION 审中-公开
标题翻译： 5保护依赖放大
公开(公告)号：WO2014009413A2
公开(公告)日：2014-01-16
申请号：PCT/EP2013/064582
申请日：2013-07-10
申请人： LEXOGEN GMBH
发明人： SEITZ, Alexander , GABLER, Irmlind , PAUL, Lukas
CPC分类号： C12N15/1096 , C12P19/34 , C12Q1/6844 , C12Q1/6858 , C12Q2521/107
摘要： The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5' protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5' protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, and optionally modifying the extension product of said nucleic acids without 5' protecting group either on the 5' end of the template strand or on the 3' end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified, wherein therefore the labelled nucleic acid did have a 5' protecting group on the RNA template strand, said 5' protecting group being optionally removed after modification, and/or labelling a complementary nucleic acid of a double strand dependent on the presence of the 5' protecting group on the complementary nucleic acids template RNA strand by double strand dependent ligation, thereby specifically generating a labelled nucleic acid complementary to an RNA at least originally comprising a 5' protecting group.
摘要翻译：本发明涉及从包含5'保护基团的RNA产生标记的核酸的方法，所述方法包括以下步骤：获得核酸模板链的混合物，所述混合物包含所述RNA和另外潜在的其它核酸，而没有 5'保护基团，至少一个寡核苷酸引物退火至所述RNA的模板链和潜在的其它核酸，以及延伸所述引物的模板序列，从而获得退火至其模板链的互补核酸链，或提供RNA 与退火至其模板链的互补核酸链双链体，并任选地在模板链的5'末端或互补链的3'末端修饰所述核酸的延伸产物，而没有5'保护基，或两者，并标记未被修饰的双链核酸的互补核酸，其中因此标记的核酸在RNA模板链上确实具有5'保护基团，所述5'保护基团在修饰后任选地被除去，和/或根据5'保护基团的存在来标记双链的互补核酸互补核酸模板RNA链通过双链依赖性连接，从而特异性产生与至少最初包含5'保护基的RNA互补的标记核酸。

7. 发明申请

WO2013038010A2 NUCLEIC ACID TRANSCRIPTION METHOD 审中-公开
标题翻译：核酸转录法
公开(公告)号：WO2013038010A2
公开(公告)日：2013-03-21
申请号：PCT/EP2012/068250
申请日：2012-09-17
申请人： LEXOGEN GMBH
发明人： SEITZ, Alexander , MOLL, Pamela , NAPORA, Magdalena Anna
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6816 , C12Q1/6853 , C12Q2521/107 , C12Q2525/113 , C12Q2533/107 , C12Q2521/501 , C12Q2525/161
摘要： The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3' end of said oligonucleotide stopper -said stopper may also be a primer itself and uses and kits for performing said method.
摘要翻译：本发明涉及产生模板核酸扩增核酸部分的方法，包括获得所述模板核酸，将至少一种寡核苷酸引物退火至所述模板核酸，将至少一种寡核苷酸终止物退火至所述模板核酸，以模板特异性方式延伸所述至少一种寡核苷酸引物直到所述延伸产物核酸达到退火的寡核苷酸终止位置，由此停止所述延伸反应，其中在所述延伸反应中所述寡核苷酸终止子不伸长，并且其中所述细长产物核酸连接到所述寡核苷酸塞子的3'末端 - 塞子塞也可以是引物本身，并且用于和执行所述方法的试剂盒。

8. 发明申请

WO2020120747A1 NUCLEIC ACID AMPLIFICATION AND IDENTIFICATION METHOD 审中-公开
公开(公告)号：WO2020120747A1
公开(公告)日：2020-06-18
申请号：PCT/EP2019/085095
申请日：2019-12-13
申请人： LEXOGEN GMBH
发明人： GÖPEL, Yvonne , MOLL, Pamela , REDA, Torsten , SEITZ, Alexander
IPC分类号： C12Q1/6855
摘要： The present invention provides a method for generating labelled amplification fragments of a nucleic acid template comprising the steps of providing said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner thereby creating an elongation product, wherein said elongating reaction stops when the elongation product reaches the 5' end of the template nucleic acid or a nucleic acid elongation stopper that is annealed to the template nucleic acid downstream of the elongation product, providing an adaptor nucleic acid that comprises an identification sequence on its 5' end,wherein said identification sequence does not hybridize to the elongation stopper when in con- tact thereto, ligating the adaptor nucleic acid at its 5' end to the 3' end of the elongation product, thereby generating a labelled amplification fragment.

9. 发明申请

WO2007062445A1 POLYNUCLEOTIDE AMPLIFICATION 审中-公开
标题翻译：多核苷酸放大
公开(公告)号：WO2007062445A1
公开(公告)日：2007-06-07
申请号：PCT/AT2006/000494
申请日：2006-11-29
申请人： SEITZ, Alexander
发明人： SEITZ, Alexander
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6844 , C12Q1/6853 , C12Q2539/113 , C12Q2521/131 , C12Q2525/179 , C12Q2521/107
摘要： The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3' end of the transcribed cDNA with a polynucleotide tail after the 3' end, c) amplification of the cDNA using a pair of primers, wherein a first (3') primer is specific for the 5' end of the cDNA and a second (51) primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3' polynucleotide tail of the cDNA.
摘要翻译：本发明提供了用于扩增样品中多核苷酸分子池的方法，其特征在于以下步骤：a）获得样品或RNA并逆转整个RNA分子，从而产生全长cDNA或获得全长cDNA样品， b）在3'末端之后用多核苷酸尾部拖尾转录的cDNA的3'末端，c）使用一对引物扩增cDNA，其中第一（3'）引物对于5'端的特异性 cDNA和第二（51）引物对于多核苷酸尾部的上游部分和cDNA的3'多核苷酸尾部上游的1至10个核苷酸是特异性的。

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