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    • 3. 发明申请
    • RNA ANALYTICS METHOD
    • RNA分析方法
    • WO2011070155A1
    • 2011-06-16
    • PCT/EP2010/069382
    • 2010-12-10
    • LEXOGEN GMBHSEITZ, AlexanderPAUL, LukasVAN MIN, Max Jan
    • SEITZ, AlexanderPAUL, LukasVAN MIN, Max Jan
    • C12Q1/68
    • C12Q1/6869C12Q1/6806C12Q1/6809C12Q2537/143C12Q2535/122C12Q2525/191C12Q2563/179C12Q2539/107
    • The present invention relates to a method of ordering nucleic acid molecule fragment sequences derived from a pool of potentially diverse RNA molecules comprising • optionally reverse transcribing the RNA molecules to provide a pool of cDNA molecules, • segregating nucleic acids from said template RNA or cDNA pool, selecting for potentially different templates with a distinctive nucleic acid feature shared by the segregated templates, thereby providing at least a first subpool of nucleic acids, • optionally once or more further segregating nucleic acids from said template RNA or cDNA, selectively segregating nucleic acids with a different distinctive nucleic acid feature, thereby providing one or more further subpool(s) of nucleic acids, • generating fragments of said segregated nucleic acid molecules by fragmenting or obtaining fragment copies of said segregated nucleic acid molecules, wherein the fragments of each subpool or combined subpools remain separable from fragments of other subpools or other combined subpools by physically separating the subpools or by attaching a label to the fragments of the subpools, with the label identifying a subpool, or determining a partial sequence of said segregated nucleic acid molecule and preferably aligning at least two sequences or partial sequences to a joined sequence.
    • 本发明涉及一种排序源自潜在不同RNA分子库的核酸分子片段序列的方法,其包括任选逆转录RNA分子以提供cDNA分子池,将核酸与所述模板RNA或cDNA库分离 选择具有由分离的模板共享的独特的核酸特征的潜在不同的模板,从而提供核酸的至少第一子池,任选地一次或多次将所述模板RNA或cDNA分离出来的核酸,选择性地将核酸与 不同的特征核酸特征,从而提供核酸的一个或多个另外的子区,通过片段化或获得所述分离的核酸分子的片段拷贝产生所述分离的核酸分子的片段,其中每个子池的片段或 组合子项目与ot的片段保持分离 她的子项目或其他组合子项目通过物理分离子项目或通过将标签附加到子项目的片段上,标签标识子项,或确定所述分离的核酸分子的部分序列,优选对齐至少两个序列或部分 序列到连接序列。