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1. 发明申请

WO2012029037A1 METHOD FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
标题翻译：放大核酸的方法
公开(公告)号：WO2012029037A1
公开(公告)日：2012-03-08
申请号：PCT/IB2011/053817
申请日：2011-08-31
申请人： KONINKLIJKE PHILIPS ELECTRONICS N.V. , FEITSMA, Harma, Martine , OVERDIJK, Marlieke, Joan , VAN DE STOLPE, Anja , VAN DER ZAAG, Pieter, Jan
发明人： FEITSMA, Harma, Martine , OVERDIJK, Marlieke, Joan , VAN DE STOLPE, Anja , VAN DER ZAAG, Pieter, Jan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2525/301
摘要： The present invention describes methods for amplifying a target nucleic acid wherein target nucleic acids hybridise to capture probe nucleic acids which are immobilized to a support via their 5 ' end. The hybridization product is further extended with a polymerase to form a double stranded nucleic acid. To this double stranded nucleic acid, a hairpin nucleic acid is ligated. This ligation product is further amplified and sequenced.
摘要翻译：本发明描述了用于扩增目标核酸的方法，其中靶核酸与通过其5'末端固定在载体上的探针核酸杂交。进一步用聚合酶扩增杂交产物以形成双链核酸。向该双链核酸中连接发夹核酸。该连接产物进一步扩增和测序。

2. 发明申请

WO2011027268A2 DEVICES AND METHODS FOR MICROARRAY SELECTION 审中-公开
标题翻译：用于微阵列选择的装置和方法
公开(公告)号：WO2011027268A2
公开(公告)日：2011-03-10
申请号：PCT/IB2010/053839
申请日：2010-08-26
申请人： KONINKLIJKE PHILIPS ELECTRONICS N.V. , VAN DER ZAAG, Pieter, Jan , FEITSMA, Harma, Martine , DEN TOONDER, Jacob, Marinus, Jan , WIMBERGER-FRIEDL, Reinhold
发明人： VAN DER ZAAG, Pieter, Jan , FEITSMA, Harma, Martine , DEN TOONDER, Jacob, Marinus, Jan , WIMBERGER-FRIEDL, Reinhold
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q2537/149 , C12Q2527/107
摘要： The present invention relates to a device for the specific selection of target molecules, comprising: (a) at least one reaction zone comprising a microarray, wherein the microarray comprises a substrate, on which one or more species of capture molecules are immobilized, comprising one or more temperature control and/or regulating units for controlling and/or regulating the temperature within the zone; (b) at least one non-reaction zone comprising one or more temperature control and/or regulating units for controlling and/or regulating the temperature within the zone, which is in fluid connection with the reaction zone; and (c) at least one transportation means capable of generating and/or regulating a fluid flow between said reaction zone (a) and said non-reaction zone comprising one or more temperature control and/or regulating units (b). The present invention further relates to a device for the specific selection of target molecules wherein the immobilized capture molecules are organized in the microarray in the form of spots, elongated spots and/or lines. In a further aspect the present invention relates to a method of specifically selecting target molecules, comprising the introducing a medium to such a device, performing interaction reactions in a reaction zone, transporting not interacted or not bound target molecules to a zone allowing reactivation of the target molecules and performing additional interaction reactions with the reactivated target molecules at the reaction zone, as well as the use of such a device for specifically selecting target molecules, e.g. for target enrichment also referred to as microarray based genome selection (MGS) in the literature.
摘要翻译：本发明涉及用于特异性选择靶分子的装置，其包含：（a）至少一个包含微阵列的反应区，其中所述微阵列包含底物，一种或多种物质捕捉分子被固定，包括一个或多个温度控制和/或调节单元，用于控制和/或调节区域内的温度; （b）至少一个非反应区，其包含一个或多个温度控制和/或调节单元，用于控制和/或调节与反应区流体连通的区域内的温度; 和（c）至少一个能够产生和/或调节所述反应区（a）和包含一个或多个温度控制和/或调节单元（b）的所述非反应区之间的流体流动的运输工具。本发明进一步涉及用于特异性选择靶分子的装置，其中固定的捕获分子以点，细长点和/或线的形式在微阵列中组织。在另一方面，本发明涉及特异性选择靶分子的方法，包括将介质引入这样的装置，在反应区中进行相互作用反应，将未相互作用或未结合的靶分子转运至允许再激活靶分子并且在反应区与再活化的靶分子进行另外的相互作用反应，以及使用这样的装置用于特异性选择靶分子，例如目标富集在文献中也被称为基于微阵列的基因组选择（MGS）。

3. 发明申请

WO2012059839A2 IN VITRO DIAGNOSTIC TESTING INCLUDING AUTOMATED BROKERING OF ROYALTY PAYMENTS FOR PROPRIETARY TESTS 审中-公开
标题翻译：在体外诊断测试中，包括自动检测专有费用的专有费用
公开(公告)号：WO2012059839A2
公开(公告)日：2012-05-10
申请号：PCT/IB2011/054735
申请日：2011-10-24
申请人： KONINKLIJKE PHILIPS ELECTRONICS N.V. , VAN DER ZAAG, Pieter, Jan , DIMITROVA, Nevenka , VAN DE STOLPE, Anja , VAN HOUTEN, Hendrik
发明人： VAN DER ZAAG, Pieter, Jan , DIMITROVA, Nevenka , VAN DE STOLPE, Anja , VAN HOUTEN, Hendrik
IPC分类号： G06Q10/00 , C12Q1/68 , G06F19/00
CPC分类号： G06Q20/10 , G06F19/00 , G06F19/18 , G06Q10/00 , G06Q50/22
摘要： A method comprises: recording an electronic record of diagnostic events including the performing of in- vitro diagnostic tests; during the recording, identifying the performing of a licensed in- vitro diagnostic test for which license information is stored in a licenses database; and computing license compensation due to a licensor for the performing of the licensed in- vitro diagnostic test based on royalty calculation information stored in the licenses database. The recording, identifying, and computing operations are performed by one or more computers. The method may further include remitting the computed license compensation to the licensor, the remitting also being performed by one or more computers. The licensed in- vitro diagnostic test may comprise a licensed biomarker test, for example one obtained by DNA or RNA sequencing, and the method may further comprise: performing the licensed biomarker test using a genome sequencer, wherein the performing of the licensed biomarker test and the computing of license compensation are both performed at a single medical facility.
摘要翻译：一种方法包括：记录诊断事件的电子记录，包括进行体外诊断测试; 在记录期间，识别许可证信息被存储在许可证数据库中的许可的体外诊断测试的执行; 以及由许可人根据许可证数据库中存储的使用费计算信息执行许可的体外诊断测试的计算许可证补偿。记录，识别和计算操作由一个或多个计算机执行。该方法还可以包括将所计算的许可证补偿递交给许可方，还由一个或多个计算机执行。许可的体外诊断测试可以包括许可的生物标志物测试，例如通过DNA或RNA测序获得的测试，并且该方法可以进一步包括：使用基因组测序仪进行许可的生物标志物测试，其中执行许可的生物标志物测试和许可证补偿的计算都在单一医疗机构进行。

4. 发明申请

WO2012127413A1 METHOD FOR PERFORMING MOLECULAR REACTIONS BY USING IMMISCIBLE INTERMEDIATE FLUIDS 审中-公开
标题翻译：通过使用可疑中间流体进行分子反应的方法
公开(公告)号：WO2012127413A1
公开(公告)日：2012-09-27
申请号：PCT/IB2012/051319
申请日：2012-03-20
申请人： KONINKLIJKE PHILIPS ELECTRONICS N.V. , WIMBERGER-FRIEDL, Reinhold , VAN DER ZAAG, Pieter, Jan , SERVOLI, Eva
发明人： WIMBERGER-FRIEDL, Reinhold , VAN DER ZAAG, Pieter, Jan , SERVOLI, Eva
IPC分类号： B01L3/00 , C12Q1/68 , B01J19/00 , G01N35/08
CPC分类号： C12Q1/6869 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/16 , B01L2300/0636 , B01L2300/088 , B01L2400/0487 , G01N35/08
摘要： The present invention relates to a method for performing molecular reactions in a device comprising the steps of (a) introducing one or more reagent solution(s) and an immiscible intermediate fluid into the device, wherein the device comprises a substrate, on which chemically or biochemically recognizable entities are immobilized; (b) performing molecular reactions between the immobilized chemically or biochemically recognizable entities and the reagent solution(s); or on the immobilized chemically or biochemically recognizable entities in the presence of the reagent solution(s); (c) displacing the reagent solution(s) present on the substrate by the immiscible intermediate fluid; (d) separating the immiscible intermediate fluid and the reagent solution(s); and reusing the reagent solution(s) and/or immiscible intermediate fluid for one or more repetitions of steps (a) to (e). The invention further relates to a device for performing a molecular reaction, comprising a reaction zone, reservoirs and liquid connections and a collection and regeneration zone wherein the immiscible intermediate fluid and the reagent solution(s) are separable by gravitational separation; or a redirection and distribution module, wherein the immiscible intermediate fluid and reagent solution(s) are separated. The invention also relates to the use of an immiscible intermediate fluid for displacing a reagent solution present in a reaction zone in a microfluidic device, as well as the use of a corresponding device for performing a sequencing reaction or a nucleic acid synthesis reaction.
摘要翻译：本发明涉及一种在器件中进行分子反应的方法，包括以下步骤：（a）将一种或多种试剂溶液和不混溶的中间体流体引入该装置，其中该装置包括基底，化学或生化鉴定实体被固定化; （b）在固定的化学或生化学可识别的实体和试剂溶液之间进行分子反应; 或在试剂溶液存在下在固定的化学或生物化学可识别的实体上; （c）通过不混溶的中间流体置换存在于基板上的试剂溶液; （d）分离不混溶的中间体液体和试剂溶液; 以及重复使用所述试剂溶液和/或不混溶的中间体流体以进行一个或多个重复的步骤（a）至（e）。本发明还涉及一种用于进行分子反应的装置，包括反应区，储存器和液体连接以及收集和再生区，其中不混溶的中间流体和试剂溶液可通过重力分离而分离; 或重定向和分配模块，其中不混溶的中间流体和试剂溶液被分离。本发明还涉及不混溶中间体液体用于置换微流体装置中反应区中存在的试剂溶液的用途，以及使用相应的装置进行测序反应或核酸合成反应。

5. 发明申请

WO2012168803A2 PROVIDING NUCLEOTIDE SEQUENCE DATA 审中-公开
标题翻译：提供核苷酸序列数据
公开(公告)号：WO2012168803A2
公开(公告)日：2012-12-13
申请号：PCT/IB2012/051920
申请日：2012-04-17
申请人： KONINKLIJKE PHILIPS ELECTRONICS N.V. , PHILIPS INTELLECTUAL PROPERTY & STANDARDS GMBH , VAN DER ZAAG, Pieter, Jan , AMBERG, Ronny , VERHAEGH, Wilhelmus, Franciscus, Johannes , BEYERLEIN, Peter , BOETTCHER, Rene
发明人： VAN DER ZAAG, Pieter, Jan , AMBERG, Ronny , VERHAEGH, Wilhelmus, Franciscus, Johannes , BEYERLEIN, Peter , BOETTCHER, Rene
IPC分类号： G06F19/20 , G06F19/22 , C12Q1/68
CPC分类号： G06F19/22 , C12Q1/6874 , G06F19/20
摘要： A sequencer device generates basic nucleotide sequence data 30 comprising probe data 34 of a capture probe in the sequencer device 10 and a determined sequence of identifiers 32 of a fragment of nucleic acids captured by the probe. The sequencer device outputs enriched nucleotide sequence data 36 that is enriched with data comprising a reference to a sequence 38 that is expected for the fragment of nucleic acids.
摘要翻译：定序器装置产生包括序列发生器装置10中的捕获探针的探针数据34的基本核苷酸序列数据30以及由探针俘获的核酸片段的确定序列32。定序器装置输出富集的核苷酸序列数据36，其富含数据，该数据包括对核酸片段预期的序列38的引用。

6. 发明申请

WO2011030272A1 DEVICE AND METHOD FOR TRANSPORTING MAGNETIC OR MAGNETISABLE BEADS 审中-公开
标题翻译：用于运输磁性或可磁化珠的装置和方法
公开(公告)号：WO2011030272A1
公开(公告)日：2011-03-17
申请号：PCT/IB2010/053991
申请日：2010-09-06
申请人： KONINKLIJKE PHILIPS ELECTRONICS N.V. , DEN TOONDER, Jacob Marinus Jan , VAN DER ZAAG, Pieter, Jan , CHAO, Hao, Chieh
发明人： DEN TOONDER, Jacob Marinus Jan , VAN DER ZAAG, Pieter, Jan , CHAO, Hao, Chieh
IPC分类号： B01L3/00 , B01F13/00 , G01N35/00
CPC分类号： B03C1/034 , B01L3/502761 , B01L2200/0668 , B01L2400/043 , B03C1/0335 , B03C1/24 , B03C2201/18
摘要： The present invention relates to a device (24) for transporting magnetic or magnetisable beads (10) over a transport surface (12). It comprises a chamber (26) comprising magnetic or magnetisable beads (10) in a fluid (28), a transport element (14) including said transport surface (12) within said chamber (26) over which said beads (10) shall be transported, a current wire structure (20) comprising at least two sets (20a, 20b, 20c) of meandering current wires arranged on a side of said transport element (14) opposite to said transport surface (12), said at least two sets (20a, 20b, 20c) being displaced with respect to each other in at least two directions, and a switching unit (32) for individually switching currents (1a, 1b, 1c) individually applied to said sets of current wires according to a current driving scheme resulting in a transport of said beads (10) over said transport surface (12). In preferred embodiments a stationary substantially uniform magnetic field (30) in a direction substantially parallel to the transport surface (12) is additionally provided.
摘要翻译：本发明涉及一种用于在输送表面（12）上输送磁性或可磁化珠（10）的装置（24）。其包括在流体（28）中包括磁性或可磁化珠（10）的腔室（26），包括在所述腔室（26）内的所述输送表面（12）的输送元件（14），所述珠子传送的电流线结构（20）包括布置在与所述输送表面（12）相对的所述输送元件（14）的一侧的曲折电流线的至少两组（20a，20b，20c），所述至少两组（20a，20b，20c）在至少两个方向上相对于彼此移位;以及开关单元（32），用于根据电流单独地切换电流（1a，1b，1c），分别施加到所述电流线组驱动方案导致所述小珠（10）在所述输送表面（12）上的运输。在优选实施例中，额外地提供了在基本上平行于输送表面（12）的方向上的固定的基本均匀的磁场（30）。

7. 发明申请

WO2007060591A2 LUMINESCENT PARTICLE AND METHOD OF DETECTING A BIOLOGICAL ENTITY USING A LUMINESCENT PARTICLE 审中-公开
标题翻译：发光颗粒和使用发光颗粒检测生物实体的方法
公开(公告)号：WO2007060591A2
公开(公告)日：2007-05-31
申请号：PCT/IB2006/054330
申请日：2006-11-20
申请人： KONINKLIJKE PHILIPS ELECTRONICS N. V. , VAN DER ZAAG, Pieter, Jan , BAKKERS, Erik, Petrus, Antonius, Maria , VAN DER MARK, Martinus, Bernardus
发明人： VAN DER ZAAG, Pieter, Jan , BAKKERS, Erik, Petrus, Antonius, Maria , VAN DER MARK, Martinus, Bernardus
CPC分类号： G01N33/588 , B82Y15/00 , G01N33/533
摘要： The invention provides a luminescent particle and a method of detecting a biological entity using a luminescent particle, the luminescent particle comprising a core area and a shell area, the core area being covered by the shell area, the core area conferring a luminescent behavior on the luminescent particle for at least one excitation wavelength and for at least one emission wavelength by means of a nanocrystal material, and the shell area being provided such that it realizes an antireflective coating of the core area.
摘要翻译：本发明提供一种发光粒子和使用发光粒子检测生物实体的方法，发光粒子包含核心区域和壳体区域，核心区域被壳体区域覆盖，核心区域赋予发光特性用于至少一个激发波长的发光粒子和通过纳米晶体材料的至少一个发射波长，并且壳体区域被设置成使得其实现核心区域的抗反射涂层。

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