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1. 发明申请

WO2013054230A3 METHOD FOR DETECTION OF COAGULATION ACTIVITY AND BIOMARKERS. 审中-公开
标题翻译：检测混凝活性和生物标记物的方法。
公开(公告)号：WO2013054230A3
公开(公告)日：2013-07-04
申请号：PCT/IB2012055286
申请日：2012-10-03
申请人： KONINKL PHILIPS ELECTRONICS NV
发明人： VAN LIESHOUT RON MARTINUS LAURENTIUS
IPC分类号： G01N33/543 , G01N33/49 , G01N33/68 , G01N33/86
CPC分类号： G01N33/54326 , G01N33/4905 , G01N33/54306 , G01N33/68 , G01N33/86
摘要： The present invention relates to a method for simultaneous detection of the coagulation activity of a blood sample and of the presence and/or amount of at least one target molecule within said blood sample, comprising the steps of: i) introducing a blood sample into a sample container comprising magnetic particles and a sensor surface, wherein said magnetic particles are functionalized with a first binding molecule, wherein said first binding molecule is attached to said magnetic particles, wherein the first binding molecule is capable of specifically binding to the at least one target molecule within said blood sample, and wherein a second binding molecule is attached to a sensor surface at the bottom of said sample container, and wherein said second binding molecule of the sensor surface is capable of specifically binding to the at least one target molecule within said blood sample; ii) measuring the presence and/or amount of said target molecule by detecting the number of magnetic particles bound to said sensor surface; wherein the number of bound magnetic particles is directly or inversely related to the amount of said at least one target molecule present in the sample; iii) at the same time or at different times measuring the coagulation activity of said blood sample, wherein said magnetic particles are magnetically actuated; and wherein the loss of mobility of said magnetic particles is detected by measuring the light reflected from said immobilized magnetic particles near or at said sensor surface; and wherein the change of detected light signal is indicative of an increase of viscosity and/or a clotting of the blood sample; and wherein the magnetic particles in steps i) to iii) are magnetically actuated using a magnetic field generator.
摘要翻译：本发明涉及用于同时检测血样的凝血活性和所述血样中至少一种靶分子的存在和/或量的方法，所述方法包括以下步骤：i）将血样引入到包括磁性颗粒和传感器表面的样品容器，其中所述磁性颗粒用第一结合分子功能化，其中所述第一结合分子附着于所述磁性颗粒，其中所述第一结合分子能够特异性结合所述至少一个靶标并且其中第二结合分子附着于所述样品容器底部的传感器表面，并且其中所述传感器表面的所述第二结合分子能够与所述血样中的所述至少一种靶分子特异性结合血液样本; ii）通过检测结合到所述传感器表面的磁性粒子的数量来测量所述靶分子的存在和/或量; 其中结合的磁性颗粒的数量与存在于所述样品中的所述至少一种目标分子的量直接或相反地相关; iii）同时或在不同时间测量所述血液样本的凝血活性，其中所述磁性粒子是磁性致动的; 并且其中通过测量从所述传感器表面附近或所述传感器表面处的所述固定磁性颗粒反射的光来检测所述磁性颗粒的迁移率的损失; 并且其中检测到的光信号的变化指示血样的粘度增加和/或凝血; 并且其中步骤i）至iii）中的磁性颗粒使用磁场发生器来磁致动。

2. 发明申请

WO2011138705A3 SENSING DEVICE FOR DETECTING A SUBSTANCE IN A FLUID 审中-公开
标题翻译：用于检测流体中的物质的感测装置
公开(公告)号：WO2011138705A3
公开(公告)日：2012-01-05
申请号：PCT/IB2011051797
申请日：2011-04-26
申请人： KONINKL PHILIPS ELECTRONICS NV , VAN LIESHOUT RON MARTINUS LAURENTIUS , PENTERMAN ROEL
发明人： VAN LIESHOUT RON MARTINUS LAURENTIUS , PENTERMAN ROEL
IPC分类号： G01N21/27 , B01L3/00 , G01N21/55 , G01N33/543
CPC分类号： G01N21/55 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L2200/027 , B01L2200/04 , B01L2200/0684 , B01L2200/143 , B01L2300/025 , B01L2300/0681 , B01L2300/0816 , B01L2300/0887 , B01L2400/0677 , G01N21/274 , G01N21/552 , G01N21/553 , G01N21/648 , G01N21/65 , G01N21/76 , G01N29/022 , G01N29/30 , G01N2021/0346 , G01N2021/7723 , G01N2201/0221 , G01N2291/0255 , G01N2291/0256 , Y10T29/49826
摘要： The invention relates to a sensing device (1) for detecting a substance (3) in a fluid (6), wherein the sensing device (1) comprises a sensing region (2) for being used for generating a sensing signal depending on the substance, a reference region (4) for being used for providing a reference signal, and a reference element (5) covering the reference region (4). The reference element (5) is adapted to shield the reference region (4) from the substance (3) and to allow the fluid (6) to penetrate the reference element (5). This reduces the influence of the reference signal by the substance (3) and, since the reference element (5) is adapted to allow the fluid (6) to penetrate the reference element (5), differences regarding properties of the fluid (6) at the sensing region (2) and of the reference element (5) at the reference region (4) can be reduced. This reduction leads to an improved reference signal, which can be used, for example, for correcting the sensing signal..
摘要翻译：本发明涉及一种用于检测流体（6）中的物质（3）的感测装置（1），其中感测装置（1）包括感测区域（2），用于根据物质产生感测信号，用于提供参考信号的参考区域（4）和覆盖参考区域（4）的参考元件（5）。参考元件（5）适于屏蔽参考区域（4）与物质（3）并允许流体（6）穿透参考元件（5）。这减少了物质（3）的参考信号的影响，并且由于参考元件（5）适于允许流体（6）穿透参考元件（5），所以关于流体（6）的性质的差异，可以减小在参考区域（4）处的感测区域（2）和参考元件（5）。该减少导致改进的参考信号，其可以用于例如用于校正感测信号。

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