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    • 6. 发明申请
    • METHOD FOR SEQUENCING A POLYNUCLEOTIDE TEMPLATE
    • 用于测序多核苷酸模板的方法
    • WO2011011175A2
    • 2011-01-27
    • PCT/US2010/040568
    • 2010-06-30
    • ILLUMINA, INC.WEST, John
    • WEST, John
    • C12Q1/68C12N15/11
    • C12Q1/6869C12Q2565/518C12Q2525/191C12Q2525/186
    • Abstract of the invention A method of determining the sequence of a target nucleic acid is provided. The method can include the steps of (a) performing a defined number of incremental extension cycles to produce a population of nucleic acid fragments having different portions of the target nucleic acid wherein the individual nucleic acid fragments in the population have a defined length that is correlated with the number of incremental extension cycles;(b) determining the sequence of the first end of individual nucleic acid fragments in the population, thereby providing first end sequences;(c) determining the sequence of the second end of individual nucleic acid fragments in the population, thereby providing second end sequences; and (d) determining the sequence of the target nucleic acid based on the first end sequences, the second end sequences and the defined length.
    • 本发明摘要提供了确定靶核酸序列的方法。 该方法可以包括以下步骤:(a)执行限定数量的增量延伸周期以产生具有靶核酸不同部分的核酸片段群体,其中群体中的各个核酸片段具有相关的限定长度 具有递增延伸周期的数目;(b)确定群体中单个核酸片段的第一末端的序列,从而提供第一末端序列;(c)确定在所述核酸片段中的每个核酸片段的第二末端序列 人口,从而提供第二结束序列; 和(d)基于第一末端序列,第二末端序列和限定的长度确定靶核酸的序列。