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1. 发明申请

WO2011056769A1 PLANTS WITH IMPROVED NITROGEN UTILIZATION AND STRESS TOLERANCE 审中-公开
标题翻译：具有改善氮肥利用和耐压性的植物
公开(公告)号：WO2011056769A1
公开(公告)日：2011-05-12
申请号：PCT/US2010/055053
申请日：2010-11-02
申请人： IOWA CORN PROMOTION BOARD , MCLAREN, James , DUCK, Nicholas , HAMMER, Philip , VANDE BERG, Brian , SCHOUTEN, Laura
发明人： MCLAREN, James , DUCK, Nicholas , HAMMER, Philip , VANDE BERG, Brian , SCHOUTEN, Laura
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine- insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻使得产量提高的限制的这些转基因植物或其组合，并且已经使用包含合成N-乙酰基的新载体构建体谷氨酸激酶（NAGK）基因，调节植物中的氮利用。本发明还包括从细菌菌株分离的精氨酸不敏感的NAGK的过表达和酶学表征，其改善胁迫耐受性和氮摄取，代谢或两者。在各种实施方案中，载体构建体包括一个或多个包括SEQ ID NO：1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

2. 发明申请

WO2008051608A2 PLANTS WITH IMPROVED NITROGEN UTILIZATION AND STRESS TOLERANCE 审中-公开
标题翻译：具有改善氮肥利用和耐压性的植物
公开(公告)号：WO2008051608A2
公开(公告)日：2008-05-02
申请号：PCT/US2007/022698
申请日：2007-10-27
申请人： IOWA CORN PROMOTION BOARD , MCLAREN, James , DUCK, Nicholas , VANDE BERG, Brian , SCHAWALDER, Alissa , BEILINSON, Vadim , HINSON, Jill
发明人： MCLAREN, James , DUCK, Nicholas , VANDE BERG, Brian , SCHAWALDER, Alissa , BEILINSON, Vadim , HINSON, Jill
IPC分类号： A01H5/00
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。在各种实施方案中，载体构建体包含一个或多个选自SEQ ID NO： 32,34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

3. 发明申请

WO2014142946A1 A PROKARYOTIC-TYPE ISOCITRATE DEHYDROGENASE AND ITS APPLICATION FOR IMPROVING NITROGEN UTILIZATION IN TRANSGENIC PLANTS 审中-公开
标题翻译：原始类型分离脱氢酶及其在转基因植物中提高氮素利用的应用
公开(公告)号：WO2014142946A1
公开(公告)日：2014-09-18
申请号：PCT/US2013/031913
申请日：2013-03-15
申请人： IOWA CORN PROMOTION BOARD , MCLAREN, James , VANDE BERG, Brian
发明人： MCLAREN, James , VANDE BERG, Brian
IPC分类号： A01H5/00
CPC分类号： C12N15/8273 , C12N9/0006 , C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic isocitrate dehydrogenase ( icdh) gene that modulates nitrogen use in plants. The invention also relates to stacking the icdh gene with other exogenous or heterologous genes that modulate nitrogen use in the plant, including a N-acetylglutamate kinase gene. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻使得产量增加的限制的这些转基因植物，或者使用包含合成的异柠檬酸脱氢酶的新型载体构建体 icdh）基因，调节植物中的氮使用。本发明还涉及将icdh基因与在植物中调节氮使用的其它外源或异源基因堆叠，包括N-乙酰谷氨酸激酶基因。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

4. 发明申请

WO2007064828A2 GRG23 AND GRG51 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GRG23和GRG51基因表达除草剂抗性
公开(公告)号：WO2007064828A2
公开(公告)日：2007-06-07
申请号：PCT/US2006/045908
申请日：2006-12-01
申请人： ATHENIX CORPORATION , PETERS, Cheryl, L. , HINSON, Jill , HAMMER, Philip, E. , VANDE BERG, Brian , SCHOUTEN, Laura, Cooper , CARR, Brian
发明人： PETERS, Cheryl, L. , HINSON, Jill , HAMMER, Philip, E. , VANDE BERG, Brian , SCHOUTEN, Laura, Cooper , CARR, Brian
IPC分类号： C12N15/54 , C12N15/82 , C12N9/10 , A01H5/00 , C12N5/10 , C12Q1/48
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds.. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO: 1 , 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性或耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含该载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中，用于在生物体（包括微生物和植物）中转化和表达。组合物还包含转化的细菌，植物，植物细胞，组织和种子。具体而言，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包含对应于多核苷酸的氨基酸序列。具体而言，本发明提供了分离的多核苷酸，其包含编码SEQ ID NO：2,4或6所示氨基酸序列或SEQ ID NO：1,3或5所示核苷酸序列的核苷酸序列。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

5. 发明申请

WO2009099906A2 DIRECTED EVOLUTION OF GRG31 AND GRG36 EPSP SYNTHASE ENZYMES 审中-公开
标题翻译： GRG31和GRG36 EPSP合成酶的指导进化
公开(公告)号：WO2009099906A2
公开(公告)日：2009-08-13
申请号：PCT/US2009/032515
申请日：2009-01-30
申请人： ATHENIX CORPORATION , HEINRICHS, Volker , SCHOUTEN, Laura, Cooper , VANDE BERG, Brian
发明人： HEINRICHS, Volker , SCHOUTEN, Laura, Cooper , VANDE BERG, Brian
IPC分类号： C12N9/10 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

6. 发明申请

WO2008105905A3 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANTS 审中-公开
公开(公告)号：WO2008105905A3
公开(公告)日：2008-09-04
申请号：PCT/US2007/073988
申请日：2007-07-20
申请人： ATHENIX CORPORATION , KOZIEL, Michael, G. , SCHOUTEN, Laura, Cooper , VANDE BERG, Brian
发明人： KOZIEL, Michael, G. , SCHOUTEN, Laura, Cooper , VANDE BERG, Brian
IPC分类号： A01H5/10 , C12N15/82 , A01H1/00
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.

7. 发明申请

WO2008100353A3 IMPROVED GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 审中-公开
公开(公告)号：WO2008100353A3
公开(公告)日：2008-08-21
申请号：PCT/US2007/085164
申请日：2007-11-20
申请人： ATHENIX CORPORATION , SCHOUTEN, Laura, Cooper , PETERS, Cheryl , VANDE BERG, Brian , HINSON, Todd
发明人： SCHOUTEN, Laura, Cooper , PETERS, Cheryl , VANDE BERG, Brian , HINSON, Todd
IPC分类号： C12N15/82 , C12N9/10 , A01H5/00 , A01H1/00
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds.. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

8. 发明申请

WO2007103768A3 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANT 审中-公开
公开(公告)号：WO2007103768A3
公开(公告)日：2007-09-13
申请号：PCT/US2007/063132
申请日：2007-03-02
申请人： ATHENIX CORPORATION , KOZIEL, Michael, G. , SCHOUTEN, Laura, Cooper , VANDE BERG, Brian
发明人： KOZIEL, Michael, G. , SCHOUTEN, Laura, Cooper , VANDE BERG, Brian
IPC分类号： A01H1/00 , A01H5/00
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.

9. 发明申请

WO2004081185A2 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
标题翻译：提高木质纤维素降解酶活性的方法
公开(公告)号：WO2004081185A2
公开(公告)日：2004-09-23
申请号：PCT/US2004/007086
申请日：2004-03-08
申请人： ATHENIX CORPORATION , BURDETTE, Jill , VANDE BERG, Brian , CARR, Brian , DUCK, Nicholas, B. , CAROZZI, Nadine , KOZIEL, Michael, G. , PATEL, Paresma, R.
发明人： BURDETTE, Jill , VANDE BERG, Brian , CARR, Brian , DUCK, Nicholas, B. , CAROZZI, Nadine , KOZIEL, Michael, G. , PATEL, Paresma, R.
IPC分类号： C12N
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译：提供了用于水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理物接触。还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。还提供了从植物材料中释放诸如酶，药物或营养制品的物质的方法。这些方法比现有方法更高效，更经济，毒性更小。

10. 发明申请

WO2003093420A3 METHODS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSE 审中-公开
公开(公告)号：WO2003093420A3
公开(公告)日：2003-11-13
申请号：PCT/US2003/013362
申请日：2003-04-30
申请人： ATHENIX CORPORATION , DUCK, Nicholas, B. , CARR, Brian , KOZIEL, Michael, G. , CAROZZI, Nadine , VANDE BERG, Brian
发明人： DUCK, Nicholas, B. , CARR, Brian , KOZIEL, Michael, G. , CAROZZI, Nadine , VANDE BERG, Brian
IPC分类号： C12P19/02
摘要： Compositions and methods for biomass conversion are provided. Compositions comprise novel enzyme mixtures that can be used directly on lignocellulose substrate. Methods involve converting lignocellulosic biomass to free sugars and small oligosaccharides with enzymes that break down lignocellulose. Novel combinations of enzymes are provided that provide a synergistic release of sugars from plant biomass. Also provided are methods to identify enzymes, strains producing enzymes, or genes that encode enzymes capable of degrading lignocellulosic material to generate sugars.

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