会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明申请
    • MOLECULAR MARKERS FOR MAPPING AND TAGGING GM8 GENE
    • GM8基因的分子标记分子标记
    • WO2005056833A1
    • 2005-06-23
    • PCT/IN2004/000355
    • 2004-11-16
    • INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGYJAIN, ArvindNAIR, SureshMOHAN, MadanKUMAR, ArvindSRIVASTAVA, M., N.
    • JAIN, ArvindNAIR, SureshMOHAN, MadanKUMAR, ArvindSRIVASTAVA, M., N.
    • C12Q1/68
    • C12Q1/6893C12Q2600/13C12Q2600/156
    • A method for tagging and mapping Gm8 , a gene conferring resistance to the rice gall midge (Orseolia oryzae), a major insect pest of rice, using AFLPs and RAPDs, onto chromosome 8 of rice is disclosed. Using AFLPs, two fragments, AR257 and AS168, were identified that were linked to the resistant and susceptible phenotypes, respectively. Another resistant phenotype-specific marker, AP 19 587 , was also identified using RAPDs. SCAR primers based on the sequence of the fragments AR257 and AS 168 failed to reveal polymorphism between the resistant and the susceptible parents. However, PCR using primers based on the regions flanking AR257 revealed polymorphism that was phenotype-specific. In contrast, PCR carried out using primers flanking the susceptible phenotype-associated fragment AS 168, produced a monomorphic fragment. Restriction digestion of these monomorphic fragments revealed polymorphism between the susceptible and resistant parents. Nucleotide BLAST searches revealed that the three fragments show strong homology to rice PAC and BAC clones that formed a contig representing the short arm of chromosome 8. PCR amplification using the above primers on a larger population, derived from a cross between two indica rice varieties, Jhitpiti (resistant parent) and TN1 (susceptible parent), showed that there is a tight linkage between the markers and the Gm8 locus.
    • 公开了一种用于标记和映射Gm8的方法,Gm8是使用AFLP和RAPDs对水稻秧苗(Orseolia oryzae)作为抗性的基因,该基因在水稻的染色体8上。 使用AFLP,分别鉴定了与抗性和易感表型相关的两个片段AR257和AS168。 还使用RAPD鉴定了另一种抗性表型特异性标记AP 19587。 基于片段AR257和AS168的序列的SCAR引物未能揭示抗性和易感亲本之间的多态性。 然而,使用基于AR257侧翼区域的引物进行PCR的PCR,显示出表型特异性的多态性。 相比之下,使用侧翼于易感表型相关片段AS 168的引物进行PCR产生单态片段。 这些单态片段的限制性消化显示易感和抗性亲本之间的多态性。 核苷酸BLAST检索显示,三个片段显示与形成代表染色体8短臂的重叠群的水稻PAC和BAC克隆的强同源性。使用来自两个a稻品种之间的杂交的较大群体的上述引物进行PCR扩增, Jhitpiti(耐药亲本)和TN1(易感亲本),表明标记与Gm8基因座之间存在紧密连锁。
    • 2. 发明申请
    • FINE MAPPING AND APPLICATION OF DNA MARKERS LINKED TO A GALL MIDGE RESISTANCE GENE FOR MARKER-AIDED SELECTION IN RICE
    • 与水稻中抗性基因连锁的水稻DNA标记精细定位及其在水稻标记辅助选择中的应用
    • WO2003033736A2
    • 2003-04-24
    • PCT/IN2002/000212
    • 2002-10-16
    • INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGYSARDESAI, NageshKUMAR, ArvindNAIR, SureshMOHAN, Madan
    • SARDESAI, NageshKUMAR, ArvindNAIR, SureshMOHAN, Madan
    • C12Q1/68
    • C07K14/415A01H1/04C12Q1/6811C12Q1/6895C12Q2600/13C12Q2600/156
    • The present invention relates to fine mapping and potential application of dna markers linked to a gall midge resistance gene, gm7 for marker-aided selection in rice. Towards this, the present invention discloses a combination of novel sequence characterized amplified region (SCAR) primers for use in assay with the DNA of Rice plants in question. A cross between the gall midge resistant parent, RP2333 carrying the Gm7 gene and susceptible parent Shyamala, is developed and a F 5 progeny is raised. A polymorphic band is identified from the F 5 progeny, using AFLP that cosegregates with the susceptible phenotype. This band is eluted from the gel and cloned. The cloned AFLP fragment is sequenced and primers are developed for selectively amplifying DNA of susceptible phenotypes, thus differentiating them from the resistant phenotypes. This Gm7 gene linked marker is mapped onto chromosome 4 of rice and is also shown to be linked to Gm2 gene and the blight resistance gene, Xal through fine mapping using Yeast Artificial Chromosomes (YACS) and cosmids. This marker is present in a single copy in the susceptible parent, Shyamala. Primers developed from this marker are able to differentiate between the resistant and susceptible phenotypes in different crosses carrying different gall midge resistance genes. A number of screenings of resistant and susceptible varieties of rice with these primers show consistent polymorphism between them. The use of primers for PCR amplification of DNAs from F 3 progenies derived from crosses between three different parental lines and the primers also differentiates the resistant phenotypes from the susceptible one. The primers of the present invention therefore have a great use in marker assisted selection as they show polymorphism between resistant and susceptible plants and therefore between plants with or without gall midge resistance genes.
    • 本发明涉及与用于标记辅助选择的gall蚊抗性基因gm7相关的dna标记的精细定位和潜在应用。 为此,本发明公开了用于与所讨论的稻植物的DNA分析的新型序列表征扩增区(SCAR)引物的组合。 产生携带Gm7基因的RP2333和敏感亲本Shyamala的抗胆虫抗性亲本之间的杂交,并产生F5子代。 使用与敏感表型共分离的AFLP从F5子代鉴定多态性条带。 该带从凝胶上洗脱并克隆。 对克隆的AFLP片段进行测序并开发引物用于选择性扩增易感表型的DNA,从而将它们与抗性表型区分开来。 这种Gm7基因连锁的标记被定位到水稻的染色体4上,并且也显示出与Gm2基因和枯萎病抗性基因Xal1连锁, 通过使用酵母人工染色体(YACS)和粘粒进行精细测绘。 这个标记在易感染父母Shyamala的单一拷贝中出现。 从这个标记开发的引物能够区分不同杂交中携带不同抗蚊基因的抗性和易感表型。 用这些引物进行的许多水稻品种的抗性和易感品种之间显示出一致的多态性。 用于PCR扩增来自三个不同亲本品系和引物之间杂交的F3代子代的DNA的PCR引物的用途也使抗性表型与易感表型相区分。 因此,本发明的引物在标记辅助选择中有很大的用途,因为它们在抗性和易感植物之间显示多态性,因此在具有或不具有gall蚊抗性基因的植物之间显示多态性。
    • 5. 发明申请
    • METHODS AND APPARATUSES FOR PROTECTING PRIVACY OF INTERNATIONAL MOBILE SUBSCRIBER IDENTITY
    • 用于保护国际移动用户身份的隐私的方法和设备
    • WO2018052409A1
    • 2018-03-22
    • PCT/US2016/051642
    • 2016-09-14
    • NOKIA SOLUTIONS AND NETWORKS OYJERICHOW, AnjaMIZIKOVSKY, SemyonNAIR, Suresh
    • JERICHOW, AnjaMIZIKOVSKY, SemyonNAIR, Suresh
    • H04W8/02H04W8/26H04W12/02
    • Systems, methods, apparatuses, and computer program products protecting the privacy of subscriber identifier(s) are provided. One method includes receiving, at a subscription server, an authentication request comprising a pseudo international mobile subscriber identity (P-IMSI) of a user equipment. The method may then include obtaining a Mobile Station PseudoNym (MSPN) from the received pseudo international mobile subscriber identity (P-IMSI), checking a local Mobile Station PseudoNym (MSPN) cache for a Mobile Station PseudoNym (MSPN) value that matches the MSPN obtained from the received P-IMSI, locating an international mobile subscriber identity (IMSI) associated with the obtained MSPN and accessing a subscription record for the IMSI, and selecting a RAND and computing a new Mobile Station PseudoNym (MSPN) value and authentication vector (AV) using the selected RAND.
    • 提供了保护用户标识符的隐私的系统,方法,装置和计算机程序产品。 一种方法包括在订阅服务器处接收包括用户设备的伪国际移动订户身份(P-IMSI)的认证请求。 该方法然后可以包括从所接收的伪国际移动订户身份(P-IMSI)获得移动站伪噪声(MSPN),针对与MSPN匹配的移动站伪噪声(MSPN)值检查本地移动站伪噪声(MSPN) 从所接收的P-IMSI中获得,定位与所获得的MSPN相关联的国际移动订户身份(IMSI),并访问IMSI的订阅记录,并选择RAND并计算新的移动台伪随机数(MSPN)值和认证向量 AV)使用所选的RAND。