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    • 2. 发明申请
    • NON-STERILE FERMENTATION OF BIOETHANOL
    • 生物柴油的非纯化发酵
    • WO2009090480A3
    • 2009-09-03
    • PCT/IB2008003536
    • 2008-12-18
    • INBICON ASLARSEN JAN
    • LARSEN JAN
    • C12P7/06C12P7/10
    • C12P7/06A01N43/08A01N61/00A01N65/00A01N65/44C12N1/22C12P7/10C12P2201/00Y02E50/16Y02E50/17
    • A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.
    • 存在一定范围的浓度,其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内,木质纤维素生物质的酵母发酵可以在非无菌条件下进行,乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现,例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗物质处理。
    • 4. 发明申请
    • NON-STERILE FERMENTATION OF BIOETHANOL
    • 生物乙醇的非无菌发酵
    • WO2009090480A4
    • 2009-11-12
    • PCT/IB2008003536
    • 2008-12-18
    • INBICON ASLARSEN JAN
    • LARSEN JAN
    • C12P7/06C12P7/10
    • C12P7/06A01N43/08A01N61/00A01N65/00A01N65/44C12N1/22C12P7/10C12P2201/00Y02E50/16Y02E50/17
    • A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.
    • 存在一系列浓度,其中源自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵性酵母。 通过优化发酵抑制剂的水平以使其落入该范围内,木质纤维素生物质的酵母发酵可以在非无菌条件下进行,其中乙醇产率与无菌条件下获得的乙醇产量相当。 通过在预处理期间和之后控制木质纤维素生物质的水/生物质比率,例如通过用预定量的淡水或回收的工艺溶液洗涤先前预处理的木质纤维素生物质的纤维部分,可以实现优化的抑制剂水平。 来自木质纤维素生物质预处理的液体馏分或工艺溶液的粗提物也可为第一代淀粉发酵提供有效的抗菌处理。